KLIFS - the kinase structures database

Version: 3.2
Updated: 29-Oct-2025

Kinase structure
Kinase:	JAK1
Family:	JakA
Group:	TK
Species:	HUMAN
IUPHAR/BPS ID:	2047
PDB:	3EYG
sc-PDB:
KIDFamMap:	Search
Release date:	2009-02-03
PubMed:	19361440
Chain:	A
(Alternate) Model:	B
Orthosteric ligand:	MI1

More entries for 3EYG
3EYG	Alternative model:	A	Chain:	A

Structural information
DFG conformation:	in
αC-helix conformation:	in
G-rich loop angle (distance):	47.4° (14.6Å)
G-rich loop rotation:	59.7°
Quality Score:	8
Resolution:	1.9 Å
Missing Residues:	0
Missing Atoms:	0

Ligand binding mode
Pockets	Subpockets	Waters
front	FP-I FP-II	Cluster I1 I2 I4 I5 I7 I11	Ligand No No No No Yes No	Protein Yes Yes No Yes No Yes

Pocket alignment
Uniprot sequence:	RDLGEGHFGKVELVAVKSLDLKKEIEILRNLYENIVKYKGIKLIMEFLPSGSLKEYLPKYLGSRQYVHRDLAARNVLVIGDFGLT
Sequence structure:	RDLGEGHFGKVELVAVKSLDLKKEIEILRNLYENIVKYKGIKLIMEFLPSGSLKEYLPKYLGSRQYVHRDLAARNVLVIGDFGLT

Ligand affinity
ChEMBL ID:	CHEMBL221959
Bioaffinities:	382 records for 35 kinases

Species	Kinase (ChEMBL naming)	Median	Min	Max	Type	Records
Homo sapiens	BMP-2-inducible protein kinase	5.2	5.2	5.2	pKd	1
Homo sapiens	CaM kinase I alpha	5.3	5.3	5.9	pKd	3
Homo sapiens	CaM kinase I delta	5.8	5.8	5.8	pKd	1
Homo sapiens	CaM kinase II alpha	5.5	5.5	5.5	pKd	1
Homo sapiens	CaM kinase II delta	5.6	5.6	5.6	pKd	1
Homo sapiens	G protein-coupled receptor kinase 7	5.4	5.4	5.4	pKd	1
Homo sapiens	Leucine-rich repeat serine/threonine-protein kinase 2	5.9	5.9	6.3	pKd	2
Homo sapiens	MAP kinase signal-integrating kinase 2	5.8	5.8	5.8	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 2	5.3	5.3	5.3	pKd	1
Homo sapiens	Myotonin-protein kinase	5.9	5.9	5.9	pKd	1
Homo sapiens	Non-receptor tyrosine-protein kinase TNK1	6.2	6.2	6.9	pKd	3
Homo sapiens	NUAK family SNF1-like kinase 2	6.4	6.4	6.6	pKd	3
Homo sapiens	Protein kinase C delta	5.7	5.7	5.7	pKd	1
Homo sapiens	Protein kinase N1	6.7	6.7	6.8	pKd	3
Homo sapiens	Protein kinase N2	5.9	5.9	5.9	pKd	1
Homo sapiens	Rho-associated protein kinase 1	6.3	6.3	6.3	pKd	1
Homo sapiens	Rho-associated protein kinase 2	5.5	5.5	5.5	pIC50	2
Homo sapiens	Rho-associated protein kinase 2	6.4	6.4	6.4	pKd	1
Homo sapiens	Ribosomal protein S6 kinase alpha 1	5.9	5.9	5.9	pKd	2
Homo sapiens	Ribosomal protein S6 kinase alpha 2	5.9	5.9	6.2	pKd	6
Homo sapiens	Ribosomal protein S6 kinase alpha 6	5.9	5.9	6.3	pKd	6
Homo sapiens	Serine/threonine-protein kinase DCLK1	5.2	5.2	5.2	pKd	1
Homo sapiens	Serine/threonine-protein kinase DCLK3	8.4	7.9	8.4	pKd	3
Homo sapiens	Serine/threonine-protein kinase MST2	5.4	5.4	5.4	pKd	2
Mycobacterium tuberculosis	Serine/threonine-protein kinase pknB	5.7	5.7	5.7	pKd	1
Homo sapiens	Serine/threonine-protein kinase ULK3	5.2	5.2	5.2	pKd	1
Homo sapiens	Tyrosine-protein kinase ABL	5.4	5.2	5.7	pKd	3
Homo sapiens	Tyrosine-protein kinase FYN	6	6	6	pKd	1
Homo sapiens	Tyrosine-protein kinase JAK1	7.3	7.3	7.4	pEC50	10
Homo sapiens	Tyrosine-protein kinase JAK1	8.5	6.9	9.3	pIC50	58
Homo sapiens	Tyrosine-protein kinase JAK1	8.8	8.8	8.8	pKd	4
Homo sapiens	Tyrosine-protein kinase JAK1	9.2	9.2	9.2	pKi	8
Homo sapiens	Tyrosine-protein kinase JAK2	7	6.7	7	pEC50	8
Homo sapiens	Tyrosine-protein kinase JAK2	7.9	5.3	9.2	pIC50	88
Homo sapiens	Tyrosine-protein kinase JAK2	8.3	8.3	9.2	pKd	8
Homo sapiens	Tyrosine-protein kinase JAK2	9.2	9	9.2	pKi	8
Mus musculus	Tyrosine-protein kinase JAK2	5.7	5.7	5.7	pIC50	1
Homo sapiens	Tyrosine-protein kinase JAK3	8.7	5.9	9.6	pIC50	62
Homo sapiens	Tyrosine-protein kinase JAK3	8.7	8.7	9.8	pKd	8
Homo sapiens	Tyrosine-protein kinase JAK3	9.4	9	9.6	pKi	10
Mus musculus	Tyrosine-protein kinase JAK3	6.8	6.8	7.3	pIC50	4
Homo sapiens	Tyrosine-protein kinase LCK	5.4	5.4	5.4	pIC50	2
Homo sapiens	Tyrosine-protein kinase LCK	5.8	5.8	6.3	pKd	2
Homo sapiens	Tyrosine-protein kinase receptor RET	5.5	5.5	5.5	pKd	1
Homo sapiens	Tyrosine-protein kinase TYK2	6.2	6.2	6.2	pEC50	4
Homo sapiens	Tyrosine-protein kinase TYK2	7.5	5.9	8.4	pIC50	26
Homo sapiens	Tyrosine-protein kinase TYK2	6.2	6.2	8.3	pKd	8
Homo sapiens	Tyrosine-protein kinase TYK2	8.1	8.1	8.4	pKi	8

Kinase-ligand interaction pattern

• Hydrophobic • Aromatic face-to-face • Aromatic face-to-edge • H-bond donor • H-bond acceptor • Ionic positive • Ionic negative

g.l

III

αC

1 R
879

2 D
880

3 L
881

4 G
882

5 E
883

6 G
884

7 H
885

8 F
886

9 G
887

10 K
888

11 V
889

12 E
890

13 L
891

14 V
905

15 A
906

16 V
907

17 K
908

18 S
909

19 L
910

20 D
921

•

αC

b.l

21 L
922

22 K
923

23 K
924

24 E
925

25 I
926

26 E
927

27 I
928

28 L
929

29 R
930

30 N
931

31 L
932

32 Y
933

33 E
935

34 N
936

35 I
937

36 V
938

37 K
939

38 Y
940

39 K
941

40 G
942

•

hinge

linker

αD

αE

41 I
943

42 K
953

43 L
954

44 I
955

45 M
956

46 E
957

47 F
958

48 L
959

49 P
960

50 S
961

51 G
962

52 S
963

53 L
964

54 K
965

55 E
966

56 Y
967

57 L
968

58 P
969

59 K
970

60 Y
993

•

••

•

αE

c.l

VII

VIII

61 L
994

62 G
995

63 S
996

64 R
997

65 Q
998

66 Y
999

67 V
1000

68 H
1001

69 R
1002

70 D
1003

71 L
1004

72 A
1005

73 A
1006

74 R
1007

75 N
1008

76 V
1009

77 L
1010

78 V
1011

79 I
1019

80 G
1020

•

DFG

a.l

81 D
1021

82 F
1022

83 G
1023

84 L
1024

85 T
1025

•

Interaction pattern search

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