KLIFS - the kinase structures database

Version: 3.2
Updated: 29-Oct-2025

ROS proto-oncogene 1 , receptor tyrosine kinase

Kinase structure
Kinase:	ROS1 (ROS)
Family:	Sev
Group:	TK
Species:	HUMAN
IUPHAR/BPS ID:	1840
PDB:	3ZBF
sc-PDB:
KIDFamMap:	Search
Release date:	2013-06-12
PubMed:	23724914
Chain:	A
Orthosteric ligand:	VGH

Structural information
DFG conformation:	in
αC-helix conformation:	in
G-rich loop angle (distance):	-
G-rich loop rotation:	-
Quality Score:	6.8
Resolution:	2.2 Å
Missing Residues:	3
Missing Atoms:	0

Ligand binding mode
Pockets	Subpockets	Waters
front	FP-I	Cluster I4 I5	Ligand No No	Protein Yes Yes

Pocket alignment
Uniprot sequence:	LLLGSGAFGEVYEVAVKTLEFLKEAHLMSKFNPNILKQLGVYIILELMEGGDLLTYLRKYLERMHFIHRDLAARNCLVIGDFGLA
Sequence structure:	LLLGSG___EVYEVAVKTLEFLKEAHLMSKFNPNILKQLGVYIILELMEGGDLLTYLRKYLERMHFIHRDLAARNCLVIGDFGLA

Ligand affinity
ChEMBL ID:	CHEMBL601719
Bioaffinities:	296 records for 146 kinases

Species	Kinase (ChEMBL naming)	Median	Min	Max	Type	Records
Homo sapiens	Activin receptor type-1	6.4	6.4	6.4	pKd	1
Homo sapiens	Activin receptor type-1B	6.1	6.1	6.1	pKd	1
Homo sapiens	Adaptor-associated kinase	5.6	5.6	5.6	pKd	1
Homo sapiens	ALK tyrosine kinase receptor	5.7	4.6	9.3	pEC50	4
Homo sapiens	ALK tyrosine kinase receptor	6.8	4.5	9.2	pIC50	53
Homo sapiens	ALK tyrosine kinase receptor	8.4	8.4	8.5	pKd	2
Homo sapiens	ALK tyrosine kinase receptor	8.1	8.1	9.1	pKi	5
Homo sapiens	AMP-activated protein kinase, alpha-1 subunit	5.6	5.6	5.6	pKd	1
Homo sapiens	Ankyrin repeat and protein kinase domain-containing protein 1	6.1	6.1	6.1	pKd	1
Homo sapiens	BMP-2-inducible protein kinase	6.1	6.1	6.1	pKd	1
Homo sapiens	Bone morphogenetic protein receptor type-1B	6.6	6.6	6.6	pKd	1
Homo sapiens	CaM-kinase kinase beta	5.8	5.8	5.8	pKd	1
Homo sapiens	Cyclin-dependent kinase 7	6.5	6.5	6.5	pKd	1
Homo sapiens	Cyclin-dependent kinase-like 2	5.7	5.7	5.7	pKd	1
Homo sapiens	Dual specificity mitogen-activated protein kinase kinase 3	5.5	5.5	5.5	pKd	1
Homo sapiens	Dual specificity testis-specific protein kinase 1	6.4	6.4	6.4	pKd	1
Homo sapiens	Dual-specificity tyrosine-phosphorylation regulated kinase 2	5.4	5.4	5.4	pKd	1
Homo sapiens	Ephrin type-A receptor 1	6.9	6.9	6.9	pKd	1
Homo sapiens	Ephrin type-A receptor 2	7	7	7	pKd	1
Homo sapiens	Ephrin type-A receptor 3	6.2	6.2	6.2	pKd	1
Homo sapiens	Ephrin type-A receptor 4	6.4	6.4	6.4	pKd	1
Homo sapiens	Ephrin type-A receptor 5	6	6	6	pKd	1
Homo sapiens	Ephrin type-A receptor 6	7.2	7.2	7.2	pKd	1
Homo sapiens	Ephrin type-A receptor 7	6.3	6.3	6.3	pKd	1
Homo sapiens	Ephrin type-A receptor 8	6.6	6.6	6.6	pKd	1
Homo sapiens	Ephrin type-B receptor 1	6.9	6.9	6.9	pKd	1
Homo sapiens	Ephrin type-B receptor 4	6.2	6.2	6.2	pKd	1
Homo sapiens	Epidermal growth factor receptor erbB1	5.6	5.6	5.6	pKd	2
Homo sapiens	Epithelial discoidin domain-containing receptor 1	6.3	6.3	6.3	pKd	1
Homo sapiens	Fibroblast growth factor receptor 3	5.5	5.5	5.6	pKd	2
Homo sapiens	Focal adhesion kinase 1	6.5	6.5	6.5	pKd	1
Homo sapiens	G protein-coupled receptor kinase 4	5.4	5.4	5.4	pKd	1
Homo sapiens	Hepatocyte growth factor receptor	8.5	7.7	9.1	pIC50	23
Homo sapiens	Hepatocyte growth factor receptor	8.8	8.7	9.7	pKd	4
Homo sapiens	Hepatocyte growth factor receptor	8.4	8.4	8.7	pKi	2
Homo sapiens	Homeodomain-interacting protein kinase 4	5.4	5.4	5.4	pKd	1
Homo sapiens	Inhibitor of nuclear factor kappa B kinase beta subunit	5.3	5.3	5.3	pKd	1
Homo sapiens	Inhibitor of nuclear factor kappa B kinase epsilon subunit	6.2	6.2	6.2	pKd	1
Homo sapiens	Insulin-like growth factor I receptor	6.8	6.8	6.8	pIC50	1
Homo sapiens	Insulin-like growth factor I receptor	6.1	6.1	6.1	pKd	1
Homo sapiens	Insulin receptor	6.2	5.5	7	pIC50	5
Homo sapiens	Insulin receptor	6.5	6.5	6.5	pKd	1
Homo sapiens	Insulin receptor-related protein	6.2	6.2	6.2	pKd	1
Homo sapiens	Interferon-induced, double-stranded RNA-activated protein kinase	6	6	6	pKd	1
Homo sapiens	Interleukin-1 receptor-associated kinase 1	7.3	7.3	7.3	pKd	1
Homo sapiens	Interleukin-1 receptor-associated kinase 3	7.5	7.5	7.5	pKd	1
Homo sapiens	Interleukin-1 receptor-associated kinase 4	5.4	5.4	5.4	pKd	1
Homo sapiens	Leukocyte tyrosine kinase receptor	7.9	7.9	7.9	pKd	1
Homo sapiens	LIM domain kinase 1	6.1	6.1	6.1	pKd	1
Homo sapiens	LIM domain kinase 2	6.2	6.2	6.2	pKd	1
Homo sapiens	Macrophage colony stimulating factor receptor	6.7	6.7	6.7	pKd	1
Homo sapiens	Macrophage-stimulating protein receptor	6.5	6.5	6.5	pIC50	1
Homo sapiens	Macrophage-stimulating protein receptor	7.6	7.6	7.6	pKd	1
Mus musculus	Macrophage-stimulating protein receptor	7.1	7.1	7.1	pIC50	1
Homo sapiens	Misshapen-like kinase 1	5.4	5.4	5.4	pKd	1
Homo sapiens	Mitogen-activated protein kinase 7	6.3	6.3	6.3	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase 1	6	6	6	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase 12	6.8	6.8	6.8	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase 13	6.6	6.6	6.6	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase 15	5.6	5.6	5.6	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase 2	7.1	7.1	7.1	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase 3	7	7	7	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase 7	5.8	5.8	5.8	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 1	7.4	7.4	7.4	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 2	7.1	7.1	7.1	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 3	7.1	7.1	7.1	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 5	7.1	7.1	7.1	pKd	1
Homo sapiens	Muscle, skeletal receptor tyrosine protein kinase	6.6	6.6	6.6	pKd	1
Homo sapiens	Myotonin-protein kinase	5.9	5.9	5.9	pKd	1
Homo sapiens	Nerve growth factor receptor Trk-A	6.2	6.2	6.2	pIC50	1
Homo sapiens	Nerve growth factor receptor Trk-A	7	7	7	pKd	1
Homo sapiens	Neurotrophic tyrosine kinase receptor type 2	6.4	6.4	8.7	pIC50	2
Homo sapiens	Neurotrophic tyrosine kinase receptor type 2	7.4	7.4	7.4	pKd	1
Homo sapiens	Non-receptor tyrosine-protein kinase TNK1	6.5	6.5	6.5	pKd	1
Homo sapiens	NT-3 growth factor receptor	7.1	7.1	7.1	pKd	1
Homo sapiens	NUAK family SNF1-like kinase 2	6.9	6.9	6.9	pKd	1
Homo sapiens	Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha	5.5	5.5	5.5	pKd	1
Homo sapiens	PITSLRE serine/threonine-protein kinase CDC2L1	6.1	6.1	6.1	pKd	1
Homo sapiens	PITSLRE serine/threonine-protein kinase CDC2L2	6.4	6.4	6.4	pKd	1
Homo sapiens	Protein kinase C mu	6	6	6	pKd	1
Homo sapiens	Protein kinase C nu	6.3	6.3	6.3	pKd	1
Homo sapiens	Protein tyrosine kinase 2 beta	6.7	6.7	6.7	pKd	1
Homo sapiens	Proto-oncogene tyrosine-protein kinase MER	8.4	8.4	8.4	pKd	1
Homo sapiens	Proto-oncogene tyrosine-protein kinase ROS	7.2	5.2	9.2	pIC50	6
Homo sapiens	Proto-oncogene tyrosine-protein kinase ROS	8.4	8.4	8.4	pKd	1
Homo sapiens	Proto-oncogene tyrosine-protein kinase ROS	8.2	6.5	9.2	pKi	3
Homo sapiens	Putative uncharacterized serine/threonine-protein kinase SgK110	6.6	6.6	6.6	pKd	1
Homo sapiens	Receptor-interacting serine/threonine-protein kinase 1	5.8	5.8	5.8	pKd	1
Homo sapiens	Receptor-interacting serine/threonine-protein kinase 4	5.7	5.7	5.7	pKd	1
Homo sapiens	Rho-associated protein kinase 1	5.4	5.4	5.4	pKd	1
Homo sapiens	Rho-associated protein kinase 2	5.5	5.5	5.5	pKd	1
Homo sapiens	Ribosomal protein S6 kinase 1	6.2	6.2	6.2	pKd	1
Homo sapiens	Serine/threonine-protein kinase 10	7.4	7.4	7.4	pKd	1
Homo sapiens	Serine/threonine-protein kinase 2	7.8	7.8	7.8	pKd	1
Homo sapiens	Serine/threonine-protein kinase 35	6.2	6.2	6.2	pKd	1
Homo sapiens	Serine/threonine-protein kinase Aurora-A	6.6	6.6	7.1	pKd	2
Homo sapiens	Serine/threonine-protein kinase Aurora-B	7.1	7.1	7.1	pKd	1
Homo sapiens	Serine/threonine-protein kinase Aurora-C	5.4	5.4	5.4	pKd	1
Homo sapiens	Serine/threonine-protein kinase DCLK1	6.5	6.5	6.5	pKd	1
Homo sapiens	Serine/threonine-protein kinase DCLK2	6.4	6.4	6.4	pKd	1
Homo sapiens	Serine/threonine-protein kinase MST1	6.2	6.2	6.2	pKd	1
Homo sapiens	Serine/threonine-protein kinase MST2	6	6	6	pKd	1
Homo sapiens	Serine/threonine-protein kinase MST4	5.6	5.6	5.6	pKd	1
Homo sapiens	Serine/threonine-protein kinase NEK7	5.2	5.2	5.2	pKd	1
Homo sapiens	Serine/threonine-protein kinase NEK9	6.1	6.1	6.1	pKd	1
Homo sapiens	Serine/threonine-protein kinase NIM1	5.3	5.3	5.3	pKd	1
Homo sapiens	Serine/threonine-protein kinase PLK4	7.2	7.2	7.2	pKd	1
Homo sapiens	Serine/threonine-protein kinase RIO1	5.2	5.2	5.2	pKd	1
Homo sapiens	Serine/threonine-protein kinase RIO3	5.4	5.4	5.4	pKd	1
Homo sapiens	Serine/threonine-protein kinase RIPK2	6.1	6.1	6.1	pKd	1
Homo sapiens	Serine/threonine-protein kinase SBK1	5.9	5.9	5.9	pKd	1
Homo sapiens	Serine/threonine-protein kinase SIK2	6.7	6.7	6.7	pKd	1
Homo sapiens	Serine/threonine-protein kinase SRPK1	5.8	5.8	5.8	pKd	1
Homo sapiens	Serine/threonine-protein kinase SRPK3	5.1	5.1	5.1	pKd	1
Homo sapiens	Serine/threonine-protein kinase TAO1	5.9	5.9	5.9	pKd	1
Homo sapiens	Serine/threonine-protein kinase TAO2	6.1	6.1	6.1	pKd	1
Homo sapiens	Serine/threonine-protein kinase TAO3	6.3	6.3	6.3	pKd	1
Homo sapiens	Serine/threonine-protein kinase TBK1	6.2	6.2	6.2	pKd	1
Homo sapiens	Serine/threonine-protein kinase TNNI3K	7	7	7	pKd	1
Homo sapiens	Serine/threonine-protein kinase ULK1	6	6	6	pKd	1
Homo sapiens	Serine/threonine-protein kinase ULK2	5.8	5.8	5.8	pKd	1
Homo sapiens	Serine/threonine-protein kinase ULK3	5.2	5.2	5.2	pKd	1
Homo sapiens	SPS1/STE20-related protein kinase YSK4	6	6	6	pKd	1
Homo sapiens	Stem cell growth factor receptor	5.4	5.4	6.1	pKd	2
Homo sapiens	TGF-beta receptor type I	6	6	6	pKd	1
Homo sapiens	Tyrosine kinase non-receptor protein 2	6.1	6.1	6.1	pKd	1
Homo sapiens	Tyrosine-protein kinase ABL	5.9	5.9	7.6	pIC50	2
Homo sapiens	Tyrosine-protein kinase ABL	7	5.5	8	pKd	15
Mus musculus	Tyrosine-protein kinase ABL	5.9	5.9	5.9	pIC50	1
Homo sapiens	Tyrosine-protein kinase ABL2	6.3	6.3	6.3	pKd	1
Homo sapiens	Tyrosine-protein kinase BLK	7	7	7	pKd	1
Homo sapiens	Tyrosine-protein kinase BMX	5.4	5.4	5.4	pKd	1
Homo sapiens	Tyrosine-protein kinase BTK	5.1	5.1	5.1	pKd	1
Homo sapiens	Tyrosine-protein kinase FER	6.6	6.6	6.6	pKd	1
Homo sapiens	Tyrosine-protein kinase FES	6.4	6.4	6.4	pKd	1
Homo sapiens	Tyrosine-protein kinase FGR	6.2	6.2	6.2	pKd	1
Homo sapiens	Tyrosine-protein kinase FRK	5.5	5.5	5.5	pKd	1
Homo sapiens	Tyrosine-protein kinase FYN	5.9	5.9	5.9	pKd	1
Homo sapiens	Tyrosine-protein kinase ITK/TSK	5.7	5.7	5.7	pKd	1
Homo sapiens	Tyrosine-protein kinase JAK1	6.5	6.5	6.5	pKd	2
Homo sapiens	Tyrosine-protein kinase JAK2	6.5	6.5	6.5	pKd	2
Homo sapiens	Tyrosine-protein kinase JAK3	6.7	6.7	6.7	pKd	2
Homo sapiens	Tyrosine-protein kinase LCK	5.6	5.6	5.6	pIC50	2
Homo sapiens	Tyrosine-protein kinase LCK	7.5	7.5	7.5	pKd	1
Homo sapiens	Tyrosine-protein kinase Lyn	6	6	6	pKd	1
Homo sapiens	Tyrosine-protein kinase receptor FLT3	6.1	5.5	6.7	pKd	7
Homo sapiens	Tyrosine-protein kinase receptor RET	5.7	5.7	5.9	pKd	2
Homo sapiens	Tyrosine-protein kinase receptor Tie-1	7	7	7	pKd	1
Homo sapiens	Tyrosine-protein kinase receptor TYRO3	6.1	6.1	6.1	pKd	1
Homo sapiens	Tyrosine-protein kinase receptor UFO	6.5	6.5	6.5	pIC50	3
Homo sapiens	Tyrosine-protein kinase receptor UFO	8.1	8.1	8.1	pKd	1
Homo sapiens	Tyrosine-protein kinase SRC	6.3	6.3	6.3	pKd	1
Homo sapiens	Tyrosine-protein kinase SYK	5.6	5.6	5.6	pKd	1
Homo sapiens	Tyrosine-protein kinase TIE-2	6.4	6.4	8.3	pIC50	2
Homo sapiens	Tyrosine-protein kinase TIE-2	6.6	6.6	6.6	pKd	1
Mus musculus	Tyrosine-protein kinase TIE-2	6.4	6.4	6.4	pIC50	1
Homo sapiens	Tyrosine-protein kinase TXK	6.1	6.1	6.1	pKd	1
Homo sapiens	Tyrosine-protein kinase TYK2	5.7	5.7	6.7	pKd	4
Homo sapiens	Tyrosine-protein kinase YES	6.1	6.1	6.1	pKd	1
Homo sapiens	Tyrosine-protein kinase ZAP-70	5.4	5.4	5.4	pKd	1
Homo sapiens	Uncharacterized aarF domain-containing protein kinase 4	6	6	6	pKd	1
Homo sapiens	Vascular endothelial growth factor receptor 1	5.6	5.6	5.6	pKd	1

Kinase-ligand interaction pattern

• Hydrophobic • Aromatic face-to-face • Aromatic face-to-edge • H-bond donor • H-bond acceptor • Ionic positive • Ionic negative

g.l

III

αC

1 L
1949

2 L
1950

3 L
1951

4 G
1952

5 S
1953

6 G
1957

7 _
_

8 _
_

9 _
_

10 E
1958

11 V
1959

12 Y
1960

13 E
1961

14 V
1977

15 A
1978

16 V
1979

17 K
1980

18 T
1981

19 L
1982

20 E
1993

•

αC

b.l

21 F
1994

22 L
1995

23 K
1996

24 E
1997

25 A
1998

26 H
1999

27 L
2000

28 M
2001

29 S
2002

30 K
2003

31 F
2004

32 N
2005

33 P
2007

34 N
2008

35 I
2009

36 L
2010

37 K
2011

38 Q
2012

39 L
2013

40 G
2014

•

hinge

linker

αD

αE

41 V
2015

42 Y
2023

43 I
2024

44 I
2025

45 L
2026

46 E
2027

47 L
2028

48 M
2029

49 E
2030

50 G
2031

51 G
2032

52 D
2033

53 L
2034

54 L
2035

55 T
2036

56 Y
2037

57 L
2038

58 R
2039

59 K
2040

60 Y
2069

•

••

•

αE

c.l

VII

VIII

61 L
2070

62 E
2071

63 R
2072

64 M
2073

65 H
2074

66 F
2075

67 I
2076

68 H
2077

69 R
2078

70 D
2079

71 L
2080

72 A
2081

73 A
2082

74 R
2083

75 N
2084

76 C
2085

77 L
2086

78 V
2087

79 I
2100

80 G
2101

•

DFG

a.l

81 D
2102

82 F
2103

83 G
2104

84 L
2105

85 A
2106

•

Interaction pattern search

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