KLIFS - the kinase structures database

Version: 3.2
Updated: 29-Oct-2025

Pim-1 proto-oncogene, serine/threonine kinase

Kinase structure
Kinase:	PIM1
Family:	PIM
Group:	CAMK
Species:	HUMAN
IUPHAR/BPS ID:	2158
PDB:	2J2I
sc-PDB:
KIDFamMap:	Search
Release date:	2007-02-13
PubMed:	18077363
Chain:	B
(Alternate) Model:	B
Orthosteric ligand:	LY4

More entries for 2J2I
2J2I	Alternative model:	A	Chain:	B

Structural information
DFG conformation:	in
αC-helix conformation:	in
G-rich loop angle (distance):	55.2° (16.7Å)
G-rich loop rotation:	40.7°
Quality Score:	8
Resolution:	1.9 Å
Missing Residues:	0
Missing Atoms:	0

Ligand binding mode
Pockets	Subpockets	Waters
front		Cluster I1 I4 I5	Ligand No No Yes	Protein Yes Yes Yes

Pocket alignment
Uniprot sequence:	PLLGSGGFGSVYSVAIKHVRVPMEVVLLKKVSSGVIRLLDWVLILERPEVQDLFDFITEHCHNCGVLHRDIKDENILILIDFGSG
Sequence structure:	PLLGSGGFGSVYSVAIKHVRVPMEVVLLKKVSSGVIRLLDWVLILERPEVQDLFDFITEHCHNCGVLHRDIKDENILILIDFGSG

Ligand affinity
ChEMBL ID:	CHEMBL91829
Bioaffinities:	252 records for 117 kinases

Species	Kinase (ChEMBL naming)	Median	Min	Max	Type	Records
Homo sapiens	3-phosphoinositide dependent protein kinase-1	6.1	6.1	6.1	pIC50	1
Homo sapiens	3-phosphoinositide dependent protein kinase-1	6.2	6.2	6.2	pKd	2
Homo sapiens	Adaptor-associated kinase	6.1	6	6.1	pKd	3
Homo sapiens	BMP-2-inducible protein kinase	6.4	5.6	6.4	pKd	3
Homo sapiens	CaM kinase I delta	5.4	5.4	5.6	pKd	2
Homo sapiens	CaM kinase II alpha	5.4	5	5.4	pKd	3
Homo sapiens	CaM kinase II delta	5.3	5.3	5.3	pKd	2
Homo sapiens	CaM kinase II gamma	5.1	5.1	5.1	pKd	2
Homo sapiens	CaM-kinase kinase alpha	5.3	5.3	5.8	pKd	3
Homo sapiens	CaM-kinase kinase beta	5.6	5.6	6	pKd	3
Homo sapiens	cAMP-dependent protein kinase alpha-catalytic subunit	5.3	5.3	5.3	pKd	2
Homo sapiens	cAMP-dependent protein kinase beta-1 catalytic subunit	5.5	5.5	5.5	pKd	2
Homo sapiens	Casein kinase II alpha	5.4	5.4	5.4	pKd	1
Homo sapiens	Casein kinase II alpha (prime)	5.2	5.2	5.5	pKd	2
Homo sapiens	cGMP-dependent protein kinase 2	5.5	5.5	5.5	pKd	2
Homo sapiens	Citron Rho-interacting kinase	5.9	5.9	5.9	pKd	2
Homo sapiens	Cyclin-dependent kinase 4	6.6	6.6	6.6	pKd	2
Homo sapiens	Cyclin-dependent kinase 7	5.8	5.8	5.8	pKd	1
Homo sapiens	Cyclin-dependent kinase-like 5	5.4	5.4	5.4	pKd	1
Homo sapiens	Death-associated protein kinase 1	6	6	6	pKd	2
Homo sapiens	Death-associated protein kinase 2	5.6	5.6	5.6	pKd	1
Homo sapiens	Death-associated protein kinase 3	5.1	5.1	5.9	pKd	2
Homo sapiens	Dual specificity mitogen-activated protein kinase kinase 4	6	6	6	pKd	2
Homo sapiens	Dual specificity mitogen-activated protein kinase kinase 5	6	6	6	pKd	1
Homo sapiens	Dual specificity mitogen-activated protein kinase kinase 6	5.4	5.4	5.4	pKd	1
Homo sapiens	Dual specificity protein kinase CLK2	6.4	6.4	6.4	pKd	2
Homo sapiens	Dual specificity protein kinase CLK4	5.7	5.1	5.7	pKd	3
Homo sapiens	Dual-specificity tyrosine-phosphorylation regulated kinase 1A	6.6	6.6	6.6	pKd	1
Homo sapiens	Dual specificity tyrosine-phosphorylation-regulated kinase 1B	5.3	5.3	5.3	pKd	2
Homo sapiens	Dual specificty protein kinase CLK1	6	6	6	pKd	2
Homo sapiens	Epidermal growth factor receptor erbB1	5.1	5.1	5.3	pKd	2
Homo sapiens	Fibroblast growth factor receptor 1	5.3	5.3	5.3	pKd	1
Homo sapiens	Glycogen synthase kinase-3 beta	5.6	5.6	5.6	pKd	2
Homo sapiens	G protein-coupled receptor kinase 4	6.2	6.2	6.2	pKd	1
Homo sapiens	G protein-coupled receptor kinase 7	6.5	6.5	6.5	pKd	1
Homo sapiens	Homeodomain-interacting protein kinase 1	6.6	6.6	6.6	pKd	1
Homo sapiens	Homeodomain-interacting protein kinase 2	6.9	6.9	6.9	pKd	1
Homo sapiens	Homeodomain-interacting protein kinase 3	7.1	7.1	7.1	pKd	1
Homo sapiens	Leucine-rich repeat serine/threonine-protein kinase 2	5	5	5	pKd	1
Homo sapiens	MAP kinase-interacting serine/threonine-protein kinase MNK1	5.2	5.2	5.2	pKd	1
Homo sapiens	MAP/microtubule affinity-regulating kinase 2	5.6	5.6	5.6	pKd	1
Homo sapiens	Misshapen-like kinase 1	6.5	6.5	6.5	pKd	1
Homo sapiens	Mitogen-activated protein kinase 15	7.1	7.1	7.1	pKd	2
Homo sapiens	Mitogen-activated protein kinase kinase kinase 2	6.2	6.2	6.2	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase 3	6.8	6.8	6.8	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase 9	5.9	5.9	5.9	pKd	2
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 1	5.6	5.6	5.6	pKd	2
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 2	6.5	6.5	6.5	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 3	5.1	5.1	5.1	pKd	2
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 5	5.1	5.1	5.1	pKd	1
Homo sapiens	Myosin light chain kinase, smooth muscle	6.5	6.5	6.5	pKd	2
Homo sapiens	Myotonin-protein kinase	6.6	6.6	6.6	pKd	2
Homo sapiens	NUAK family SNF1-like kinase 1	5.8	5.8	5.8	pKd	2
Homo sapiens	NUAK family SNF1-like kinase 2	6.5	6.5	6.5	pKd	2
Homo sapiens	Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha	6.2	6.2	6.2	pKd	2
Homo sapiens	Phosphatidylinositol-5-phosphate 4-kinase type-2 beta	6.1	6.1	6.1	pKd	2
Homo sapiens	Phosphorylase kinase gamma subunit 1	5.4	5.4	5.6	pKd	3
Homo sapiens	Platelet-derived growth factor receptor beta	5.2	5.2	5.2	pKd	1
Homo sapiens	Protein kinase C alpha	6.4	6.4	6.4	pIC50	3
Homo sapiens	Protein kinase C beta	8.2	8.2	8.3	pIC50	7
Homo sapiens	Protein kinase C delta	6.6	6.6	6.6	pIC50	1
Homo sapiens	Protein kinase C delta	8.4	8.4	8.4	pKd	2
Homo sapiens	Protein kinase C epsilon	6.2	6.2	6.2	pIC50	2
Homo sapiens	Protein kinase C epsilon	8	8	8	pKd	2
Homo sapiens	Protein kinase C eta	7.3	7.3	7.3	pIC50	1
Homo sapiens	Protein kinase C eta	5.8	5.8	5.8	pKd	2
Homo sapiens	Protein kinase C gamma	6.5	6.5	6.5	pIC50	3
Homo sapiens	Protein kinase C iota	5.9	5.9	5.9	pKd	1
Homo sapiens	Protein kinase C theta	8.6	8.6	8.6	pKd	2
Homo sapiens	Protein kinase N1	6.5	6.5	6.5	pKd	2
Homo sapiens	Protein kinase N2	5.8	5.8	5.8	pKd	2
Homo sapiens	Proto-oncogene tyrosine-protein kinase ROS	5.2	5.2	5.2	pKd	1
Homo sapiens	Rhodopsin kinase	6.1	6.1	6.1	pKd	1
Homo sapiens	Ribosomal protein S6 kinase 1	6.7	6.7	6.7	pKd	1
Homo sapiens	Ribosomal protein S6 kinase alpha 1	6.4	6.4	6.4	pKd	4
Homo sapiens	Ribosomal protein S6 kinase alpha 2	6.6	5.8	6.6	pKd	6
Homo sapiens	Ribosomal protein S6 kinase alpha 3	5.4	5.4	5.7	pKd	6
Homo sapiens	Ribosomal protein S6 kinase alpha 4	5.2	5.2	5.2	pKd	4
Homo sapiens	Ribosomal protein S6 kinase alpha 5	5.9	5.7	5.9	pKd	6
Homo sapiens	Ribosomal protein S6 kinase alpha 6	5.6	5.6	5.6	pKd	4
Homo sapiens	Serine/threonine-protein kinase 10	6.6	6	6.6	pKd	3
Homo sapiens	Serine/threonine-protein kinase 11	5.2	5.2	5.2	pKd	1
Homo sapiens	Serine/threonine-protein kinase 2	6.4	6	6.4	pKd	3
Homo sapiens	Serine/threonine-protein kinase 33	5.5	5.5	5.6	pKd	2
Homo sapiens	Serine/threonine-protein kinase AKT2	5.1	5.1	5.1	pKd	1
Homo sapiens	Serine/threonine-protein kinase AKT3	5.9	5.9	5.9	pKd	2
Homo sapiens	Serine/threonine-protein kinase Aurora-C	5.5	5.5	5.5	pKd	1
Homo sapiens	Serine/threonine-protein kinase Chk1	6.3	6.3	6.3	pKd	2
Homo sapiens	Serine/threonine-protein kinase Chk2	5.4	5.4	5.4	pKd	1
Homo sapiens	Serine/threonine-protein kinase D2	6.1	6.1	6.1	pKd	2
Homo sapiens	Serine/threonine-protein kinase GAK	6	5.9	6	pKd	3
Homo sapiens	Serine/threonine-protein kinase ICK	6.6	6.6	6.6	pKd	1
Homo sapiens	Serine/threonine-protein kinase LATS1	5.6	5.6	5.6	pKd	2
Homo sapiens	Serine/threonine-protein kinase LATS2	6.2	6.2	6.2	pKd	2
Homo sapiens	Serine/threonine-protein kinase MST1	6.7	6.7	6.7	pKd	1
Homo sapiens	Serine/threonine-protein kinase MST2	6	6	6	pKd	2
Mus musculus	Serine/threonine-protein kinase MST2	7.1	7.1	7.1	pKd	1
Homo sapiens	Serine/threonine-protein kinase PCTAIRE-1	5.1	5.1	5.6	pKd	2
Homo sapiens	Serine/threonine-protein kinase PIM1	6.7	6.7	6.7	pIC50	2
Homo sapiens	Serine/threonine-protein kinase PIM1	6.6	6.6	7.3	pKd	4
Homo sapiens	Serine/threonine-protein kinase PIM2	5.8	5.8	6.9	pKd	4
Homo sapiens	Serine/threonine-protein kinase PIM3	7.9	7.9	7.9	pKd	2
Homo sapiens	Serine/threonine-protein kinase PLK4	5.3	5.3	6.4	pKd	3
Homo sapiens	Serine/threonine-protein kinase PRP4 homolog	5.8	5.8	5.8	pKd	1
Homo sapiens	Serine/threonine-protein kinase RIO1	6.1	6.1	6.1	pKd	2
Homo sapiens	Serine/threonine-protein kinase RIO3	6.2	6.2	6.2	pKd	1
Homo sapiens	Serine/threonine-protein kinase Sgk3	5.9	5.9	5.9	pKd	1
Homo sapiens	Serine/threonine-protein kinase SRPK1	5.9	5.9	5.9	pKd	1
Homo sapiens	Serine/threonine-protein kinase SRPK2	6	6	6	pKd	1
Homo sapiens	Serine/threonine-protein kinase SRPK3	5.8	5.8	5.8	pKd	1
Homo sapiens	Serine/threonine-protein kinase TAO1	5.8	5.8	5.8	pKd	1
Homo sapiens	Serine/threonine-protein kinase TAO2	5.4	5.4	5.4	pKd	1
Homo sapiens	Serine/threonine-protein kinase TAO3	7	7	7	pKd	1
Homo sapiens	Serine/threonine-protein kinase TBK1	6	6	6	pKd	1
Homo sapiens	Serine/threonine-protein kinase tousled-like 1	5.7	5.7	5.7	pKd	1
Homo sapiens	SPS1/STE20-related protein kinase YSK4	7.3	7.3	7.3	pKd	1
Homo sapiens	Stem cell growth factor receptor	5.8	5	6.4	pKd	8
Homo sapiens	TRAF2- and NCK-interacting kinase	5.2	5.2	5.2	pKd	1
Homo sapiens	Tyrosine-protein kinase HCK	5.6	5.6	5.6	pKd	1
Homo sapiens	Tyrosine-protein kinase JAK3	5.9	5.9	6.4	pKd	4
Homo sapiens	Tyrosine-protein kinase LCK	5.9	5.9	5.9	pKd	1
Homo sapiens	Tyrosine-protein kinase receptor FLT3	6.6	5.7	6.9	pKd	13

Kinase-ligand interaction pattern

• Hydrophobic • Aromatic face-to-face • Aromatic face-to-edge • H-bond donor • H-bond acceptor • Ionic positive • Ionic negative

g.l

III

αC

1 P
42

2 L
43

3 L
44

4 G
45

5 S
46

6 G
47

7 G
48

8 F
49

9 G
50

10 S
51

11 V
52

12 Y
53

13 S
54

14 V
64

15 A
65

16 I
66

17 K
67

18 H
68

19 V
69

20 R
85

•

••

•

αC

b.l

21 V
86

22 P
87

23 M
88

24 E
89

25 V
90

26 V
91

27 L
92

28 L
93

29 K
94

30 K
95

31 V
96

32 S
97

33 S
101

34 G
102

35 V
103

36 I
104

37 R
105

38 L
106

39 L
107

40 D
108

•

hinge

linker

αD

αE

41 W
109

42 V
117

43 L
118

44 I
119

45 L
120

46 E
121

47 R
122

48 P
123

49 E
124

50 V
126

51 Q
127

52 D
128

53 L
129

54 F
130

55 D
131

56 F
132

57 I
133

58 T
134

59 E
135

60 H
157

•

αE

c.l

VII

VIII

61 C
158

62 H
159

63 N
160

64 C
161

65 G
162

66 V
163

67 L
164

68 H
165

69 R
166

70 D
167

71 I
168

72 K
169

73 D
170

74 E
171

75 N
172

76 I
173

77 L
174

78 I
175

79 L
184

80 I
185

•

DFG

a.l

81 D
186

82 F
187

83 G
188

84 S
189

85 G
190

•

Interaction pattern search

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