KLIFS - the kinase structures database

Version: 3.2
Updated: 29-Oct-2025

Mitogen-activated protein kinase-activated protein kinase 3

Kinase structure
Kinase:	MAPKAPK3
Family:	MAPKAPK
Group:	CAMK
Species:	HUMAN
IUPHAR/BPS ID:	2095
PDB:	3FHR
sc-PDB:
KIDFamMap:	Search
Release date:	2009-12-15
PubMed:	19937655
Chain:	A
(Alternate) Model:	A
Orthosteric ligand:	P4O

More entries for 3FHR
3FHR	Alternative model:	B	Chain:	A

Structural information
DFG conformation:	in
αC-helix conformation:	in
G-rich loop angle (distance):	48° (14.2Å)
G-rich loop rotation:	80°
Quality Score:	8
Resolution:	1.9 Å
Missing Residues:	0
Missing Atoms:	0

Ligand binding mode
Pockets	Subpockets	Waters
front		Cluster I1 I2 I4 I5 I9	Ligand Yes No No Yes Yes	Protein No Yes Yes Yes Yes

Pocket alignment
Uniprot sequence:	QVLGLGVNGKVLECALKLLKARQEVDHHWQASPHIVCILDVLIIMECMEGGELFSRIQEFLHSHNIAHRDVKPENLLYLTDFGFA
Sequence structure:	QVLGLGVNGKVLECALKLLKARQEVDHHWQASPHIVCILDVLIIMECMEGGELFSRIQEFLHSHNIAHRDVKPENLLYLTDFGFA

Ligand affinity
ChEMBL ID:	CHEMBL226403
Bioaffinities:	48 records for 41 kinases

Species	Kinase (ChEMBL naming)	Median	Min	Max	Type	Records
Homo sapiens	AMP-activated protein kinase, alpha-1 subunit	6	6	6	pKi	1
Homo sapiens	BR serine/threonine-protein kinase 1	5.6	5.6	5.6	pKi	1
Homo sapiens	CaM kinase II beta	5.2	5.2	5.2	pKi	1
Homo sapiens	CaM kinase II delta	6.1	6.1	6.1	pKi	1
Homo sapiens	CaM kinase II gamma	5.4	5.4	5.4	pKi	1
Homo sapiens	Casein kinase I alpha	6	6	6	pKi	1
Homo sapiens	Casein kinase I delta	6.7	6.7	6.7	pKi	1
Homo sapiens	Casein kinase I gamma 1	6.6	6.6	6.6	pKi	1
Homo sapiens	Casein kinase I gamma 2	6.5	6.5	6.5	pKi	1
Homo sapiens	Casein kinase II alpha	6.2	6.2	6.2	pKi	1
Homo sapiens	c-Jun N-terminal kinase 1	6.5	6.5	6.5	pKi	1
Homo sapiens	c-Jun N-terminal kinase 2	6.4	6.4	6.4	pKi	1
Homo sapiens	Cyclin-dependent kinase 1	5.4	5.4	5.4	pKi	1
Homo sapiens	Death-associated protein kinase 3	7.6	7.6	7.6	pKi	1
Homo sapiens	Dual specificity protein kinase CLK2	5.9	5.9	5.9	pKi	1
Homo sapiens	Dual specificity protein kinase CLK4	7.2	7.2	7.2	pKi	1
Homo sapiens	Dual-specificity tyrosine-phosphorylation regulated kinase 1A	8.4	8.4	8.4	pKi	1
Homo sapiens	Dual-specificity tyrosine-phosphorylation regulated kinase 3	7.1	7.1	7.1	pKi	1
Homo sapiens	Dual specificity tyrosine-phosphorylation-regulated kinase 4	6.5	6.5	6.5	pKi	1
Homo sapiens	Interleukin-1 receptor-associated kinase 1	5.3	5.3	5.3	pKi	1
Homo sapiens	MAP kinase-activated protein kinase 2	7.3	7.3	7.3	pEC50	1
Homo sapiens	MAP kinase-activated protein kinase 2	8.1	8.1	8.1	pIC50	2
Homo sapiens	MAP kinase-activated protein kinase 2	7.5	7.5	7.5	pKi	1
Homo sapiens	MAP kinase-activated protein kinase 3	6.7	6.7	6.7	pIC50	1
Homo sapiens	MAP kinase-activated protein kinase 3	6.8	6.8	6.8	pKi	1
Homo sapiens	MAP kinase-activated protein kinase 5	7	7	7.1	pIC50	2
Homo sapiens	MAP kinase-activated protein kinase 5	7.2	7.2	7.2	pKi	1
Homo sapiens	MAP kinase ERK2	5.5	5.5	5.5	pIC50	1
Homo sapiens	MAP kinase ERK2	6.4	6.4	6.4	pKi	1
Homo sapiens	MAP kinase-interacting serine/threonine-protein kinase MNK1	5.2	5.2	5.2	pIC50	1
Homo sapiens	MAP kinase p38 gamma	5.3	5.3	5.3	pKi	1
Homo sapiens	MAP kinase signal-integrating kinase 2	6	6	6	pKi	1
Homo sapiens	Misshapen-like kinase 1	5.5	5.5	5.5	pKi	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 2	5.7	5.7	5.7	pKi	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 4	6.8	6.8	6.8	pKi	1
Homo sapiens	Protein kinase C nu	6.6	6.6	6.6	pKi	1
Homo sapiens	Ribosomal protein S6 kinase 1	6	6	6	pKi	1
Homo sapiens	Serine/threonine-protein kinase 17A	7.2	7.2	7.2	pKi	1
Homo sapiens	Serine/threonine-protein kinase Aurora-B	6.2	6.2	6.2	pKi	1
Homo sapiens	Serine/threonine-protein kinase Chk2	6.8	6.8	6.8	pKi	1
Homo sapiens	Serine/threonine-protein kinase D2	7.4	7.4	7.4	pKi	1
Homo sapiens	Serine/threonine-protein kinase PIM1	7.1	7.1	7.1	pKi	1
Homo sapiens	Serine/threonine-protein kinase PIM2	6.4	6.4	6.4	pKi	1
Homo sapiens	Serine/threonine-protein kinase PIM3	7.5	7.5	7.5	pKi	1
Homo sapiens	Serine/threonine-protein kinase PLK4	7.2	7.2	7.2	pKi	1
Homo sapiens	Serine/threonine-protein kinase SRPK1	5.4	5.4	5.4	pKi	1

Kinase-ligand interaction pattern

• Hydrophobic • Aromatic face-to-face • Aromatic face-to-edge • H-bond donor • H-bond acceptor • Ionic positive • Ionic negative

g.l

III

αC

1 Q
48

2 V
49

3 L
50

4 G
51

5 L
52

6 G
53

7 V
54

8 N
55

9 G
56

10 K
57

11 V
58

12 L
59

13 E
60

14 C
70

15 A
71

16 L
72

17 K
73

18 L
74

19 L
75

20 K
80

•

••

αC

b.l

21 A
81

22 R
82

23 Q
83

24 E
84

25 V
85

26 D
86

27 H
87

28 H
88

29 W
89

30 Q
90

31 A
91

32 S
92

33 P
95

34 H
96

35 I
97

36 V
98

37 C
99

38 I
100

39 L
101

40 D
102

hinge

linker

αD

αE

41 V
103

42 L
115

43 I
116

44 I
117

45 M
118

46 E
119

47 C
120

48 M
121

49 E
122

50 G
123

51 G
124

52 E
125

53 L
126

54 F
127

55 S
128

56 R
129

57 I
130

58 Q
131

59 E
132

60 F
156

•

••

•

αE

c.l

VII

VIII

61 L
157

62 H
158

63 S
159

64 H
160

65 N
161

66 I
162

67 A
163

68 H
164

69 R
165

70 D
166

71 V
167

72 K
168

73 P
169

74 E
170

75 N
171

76 L
172

77 L
173

78 Y
174

79 L
185

80 T
186

•

DFG

a.l

81 D
187

82 F
188

83 G
189

84 F
190

85 A
191

•

Interaction pattern search

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