Version: 3.2
Updated: 29-Oct-2025
Mitogen-activated protein kinase-activated protein kinase 3
Kinase structure
Kinase:MAPKAPK3
Family:MAPKAPK
Group:CAMK
Species:HUMAN
IUPHAR/BPS ID:2095
PDB:3FXW
sc-PDB:
KIDFamMap:Search
Release date:2010-01-26
Chain:A
(Alternate) Model:B
Orthosteric ligand:F10
2D structure of the orthosteric ligand
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The orthosteric binding pocket


More entries for 3FXW
3FXWAlternative model: AChain: A


Structural information
DFG conformation:in
αC-helix conformation:in
G-rich loop angle (distance):48.1° (14.4Å)
G-rich loop rotation:72.3°
Quality Score:7.6
Resolution:2 Å
Missing Residues:1
Missing Atoms:0
Ligand binding mode
PocketsSubpocketsWaters
front
Cluster
I1
I2
I4
I5
I9
Ligand
No
No
No
Yes
Yes
Protein
No
Yes
Yes
Yes
Yes

Pocket alignment
Uniprot sequence:QVLGLGVNGKVLECALKLLKARQEVDHHWQASPHIVCILDVLIIMECMEGGELFSRIQEFLHSHNIAHRDVKPENLLYLTDFGFA
Sequence structure:QVLGLGV_GKVLECALKLLKARQEVDHHWQASPHIVCILDVLIIMECMEGGELFSRIQEFLHSHNIAHRDVKPENLLYLTDFGFA

Ligand affinity
ChEMBL ID:CHEMBL226471
Bioaffinities: 58 records for 46 kinases
Species Kinase (ChEMBL naming) Median Min Max Type Records
Homo sapiensAMP-activated protein kinase, alpha-1 subunit5.85.85.8pKi1
Homo sapiensBR serine/threonine-protein kinase 15.95.95.9pKi1
Homo sapiensCasein kinase I alpha5.75.75.7pKi1
Homo sapiensCasein kinase I delta666pKi1
Homo sapiensCasein kinase I gamma 16.26.26.2pKi1
Homo sapiensCasein kinase I gamma 26.86.86.8pKi1
Homo sapiensCasein kinase II alpha5.85.85.8pKi1
Homo sapiensc-Jun N-terminal kinase 16.86.86.8pKi1
Homo sapiensc-Jun N-terminal kinase 24.74.74.7pIC501
Homo sapiensc-Jun N-terminal kinase 26.56.56.5pKi1
Homo sapiensCyclin-dependent kinase 14.54.54.5pIC501
Homo sapiensCyclin-dependent kinase 15.55.55.5pKi1
Homo sapiensDeath-associated protein kinase 36.26.26.2pKi1
Homo sapiensDual specificity protein kinase CLK25.95.95.9pKi1
Homo sapiensDual specificity protein kinase CLK46.56.56.5pKi1
Homo sapiensDual-specificity tyrosine-phosphorylation regulated kinase 1A888pKi1
Homo sapiensDual-specificity tyrosine-phosphorylation regulated kinase 36.76.76.7pKi1
Homo sapiensEpidermal growth factor receptor erbB16.26.26.2pKi1
Homo sapiensGlycogen synthase kinase-3 alpha5.45.45.4pKi1
Homo sapiensHepatocyte growth factor receptor5.95.95.9pKi1
Homo sapiensInterleukin-1 receptor-associated kinase 15.95.95.9pKi1
Homo sapiensMAP kinase-activated protein kinase 25.65.66.4pEC502
Homo sapiensMAP kinase-activated protein kinase 25.45.16.9pIC504
Homo sapiensMAP kinase-activated protein kinase 3666pIC501
Homo sapiensMAP kinase-activated protein kinase 35.65.65.6pKi1
Homo sapiensMAP kinase-activated protein kinase 56.76.76.7pIC501
Homo sapiensMAP kinase-activated protein kinase 56.66.66.6pKi1
Homo sapiensMAP kinase ERK25.35.35.3pIC501
Homo sapiensMAP kinase ERK25.85.85.8pKi1
Homo sapiensMAP kinase p38 alpha4.14.14.1pIC501
Homo sapiensMAP kinase p38 delta5.85.85.8pKi1
Homo sapiensMAP kinase p38 gamma5.75.75.7pKi1
Homo sapiensMAP kinase signal-integrating kinase 25.85.85.8pKi1
Homo sapiensMaternal embryonic leucine zipper kinase5.85.85.8pKi1
Homo sapiensMisshapen-like kinase 16.66.66.6pKi1
Homo sapiensMitogen-activated protein kinase kinase kinase kinase 2666pKi1
Homo sapiensMitogen-activated protein kinase kinase kinase kinase 47.67.67.6pKi1
Homo sapiensMitogen-activated protein kinase kinase kinase kinase 56.26.26.2pKi1
Homo sapiensProtein kinase C nu6.56.56.5pKi1
Homo sapiensRibosomal protein S6 kinase 1666pKi1
Homo sapiensRibosomal protein S6 kinase alpha 44.24.24.2pIC502
Homo sapiensSerine/threonine-protein kinase 25.45.45.4pKi1
Homo sapiensSerine/threonine-protein kinase Aurora-B5.25.25.2pKi1
Homo sapiensSerine/threonine-protein kinase Chk26.26.26.2pKi1
Homo sapiensSerine/threonine-protein kinase D26.66.66.6pKi1
Homo sapiensSerine/threonine-protein kinase Nek45.25.25.2pKi1
Homo sapiensSerine/threonine-protein kinase PIM16.16.16.1pKi1
Homo sapiensSerine/threonine-protein kinase PLK46.16.16.1pKi1
Homo sapiensSerine/threonine-protein kinase SRPK15.45.45.4pKi1
Homo sapiensTyrosine-protein kinase JAK36.36.36.3pIC502
Homo sapiensTyrosine-protein kinase receptor UFO5.85.85.8pKi1
Homo sapiensVascular endothelial growth factor receptor 25.15.15.1pKi1


Kinase-ligand interaction pattern
• Hydrophobic • Aromatic face-to-face • Aromatic face-to-edge • H-bond donor • H-bond acceptor • Ionic positive • Ionic negative
I g.l II III αC
1 Q
48		2 V
49		3 L
50		4 G
51		5 L
52		6 G
53		7 V
54		8 _
_		9 G
56		10 K
57		11 V
58		12 L
59		13 E
60		14 C
70		15 A
71		16 L
72		17 K
73		18 L
74		19 L
75		20 K
80
••••••••
αC b.l IV
21 A
81		22 R
82		23 Q
83		24 E
84		25 V
85		26 D
86		27 H
87		28 H
88		29 W
89		30 Q
90		31 A
91		32 S
92		33 P
95		34 H
96		35 I
97		36 V
98		37 C
99		38 I
100		39 L
101		40 D
102
•
IV V GK hinge linker αD αE
41 V
103		42 L
115		43 I
116		44 I
117		45 M
118		46 E
119		47 C
120		48 M
121		49 E
122		50 G
123		51 G
124		52 E
125		53 L
126		54 F
127		55 S
128		56 R
129		57 I
130		58 Q
131		59 E
132		60 F
156
•••••••
αE VI c.l VII VIII x
61 L
157		62 H
158		63 S
159		64 H
160		65 N
161		66 I
162		67 A
163		68 H
164		69 R
165		70 D
166		71 V
167		72 K
168		73 P
169		74 E
170		75 N
171		76 L
172		77 L
173		78 Y
174		79 L
185		80 T
186
••
DFG a.l
81 D
187		82 F
188		83 G
189		84 F
190		85 A
191
••

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