KLIFS - the kinase structures database

Version: 3.2
Updated: 29-Oct-2025

Kinase structure
Kinase:	EPHA2 (EphA2)
Family:	Eph
Group:	TK
Species:	HUMAN
IUPHAR/BPS ID:	1822
PDB:	5IA4
sc-PDB:
KIDFamMap:	Search
Release date:	2016-11-09
PubMed:	27685543
Chain:	A
(Alternate) Model:	A
Orthosteric ligand:	88Z

More entries for 5IA4
5IA4	Alternative model:	B	Chain:	A

Structural information
DFG conformation:	out
αC-helix conformation:	in
G-rich loop angle (distance):	66.6° (20.8Å)
G-rich loop rotation:	44°
Quality Score:	8
Resolution:	1.8 Å
Missing Residues:	0
Missing Atoms:	0

Ligand binding mode
Pockets	Subpockets	Waters
front gate back	BP-I-A BP-I-B BP-II-out BP-III	Cluster I1	Ligand No	Protein Yes

Pocket alignment
Uniprot sequence:	KVIGAGEFGEVYKVAIKTLDFLGEAGIMGQFSHNIIRLEGVMIITEYMENGALDKFLREYLANMNYVHRDLAARNILVVSDFGLS
Sequence structure:	KVIGAGEFGEVYKVAIKTLDFLGEAGIMGQFSHNIIRLEGVMIITEYMENGALDKFLREYLANMNYVHRDLAARNILVVSDFGLS

Ligand affinity
ChEMBL ID:	CHEMBL1230609
Bioaffinities:	335 records for 188 kinases

Species	Kinase (ChEMBL naming)	Median	Min	Max	Type	Records
Homo sapiens	ALK tyrosine kinase receptor	7.2	7.2	7.2	pKd	1
Homo sapiens	AMP-activated protein kinase, alpha-1 subunit	6.1	6.1	6.1	pKd	1
Homo sapiens	AMP-activated protein kinase, alpha-2 subunit	5.9	5.9	5.9	pKd	1
Homo sapiens	Ankyrin repeat and protein kinase domain-containing protein 1	5.8	5.8	5.8	pKd	1
Plasmodium falciparum (isolate 3D7)	Calcium-dependent protein kinase 1	6.6	6.6	6.6	pKd	1
Homo sapiens	CaM kinase I alpha	5.2	5.2	5.2	pKd	1
Homo sapiens	CaM kinase I delta	5.3	5.3	5.3	pKd	1
Homo sapiens	CaM-kinase kinase alpha	6.3	6.3	6.3	pKd	1
Homo sapiens	CaM-kinase kinase beta	5.4	5.4	5.4	pKd	1
Homo sapiens	Casein kinase I epsilon	5.3	5.3	5.3	pKd	1
Homo sapiens	Casein kinase II alpha (prime)	5.6	5.6	5.6	pKd	1
Homo sapiens	Cell division cycle 2-like protein kinase 6	7.1	7.1	7.1	pKd	1
Homo sapiens	Cell division protein kinase 8	6.9	6.9	6.9	pKd	1
Homo sapiens	Citron Rho-interacting kinase	5.5	5.5	5.5	pKd	1
Homo sapiens	c-Jun N-terminal kinase 1	5.2	5.2	5.2	pKd	1
Homo sapiens	c-Jun N-terminal kinase 2	6.2	6.2	6.2	pKd	1
Homo sapiens	c-Jun N-terminal kinase 3	5.4	5.4	5.4	pKd	1
Homo sapiens	Cyclin-dependent kinase 7	8.4	8.4	8.4	pKd	1
Homo sapiens	Cyclin-dependent kinase 9	5.8	5.8	5.8	pKd	1
Homo sapiens	Cyclin-dependent kinase-like 2	6.6	6.6	6.6	pKd	1
Homo sapiens	Cyclin-dependent kinase-like 3	5.4	5.4	5.4	pKd	1
Homo sapiens	Cyclin-dependent kinase-like 5	5.6	5.6	5.6	pKd	1
Homo sapiens	Death-associated protein kinase 3	5.5	5.5	5.5	pKd	1
Homo sapiens	Discoidin domain-containing receptor 2	8.4	8.4	8.4	pKd	1
Homo sapiens	Dual specificity mitogen-activated protein kinase kinase 1	7.9	7.9	7.9	pKd	1
Homo sapiens	Dual specificity mitogen-activated protein kinase kinase 2	7.9	7.9	7.9	pKd	1
Homo sapiens	Dual specificity mitogen-activated protein kinase kinase 5	8.6	8.6	8.6	pKd	1
Homo sapiens	Dual specificity protein kinase CLK4	6.1	6.1	6.1	pKd	1
Homo sapiens	Dual specificity protein kinase TTK	6.4	6.4	6.4	pKd	1
Homo sapiens	Dual specificity testis-specific protein kinase 1	6.6	6.6	6.6	pKd	1
Homo sapiens	Dual-specificity tyrosine-phosphorylation regulated kinase 2	5.7	5.7	5.7	pKd	1
Homo sapiens	Dual specificty protein kinase CLK1	6.3	6.3	6.3	pKd	1
Homo sapiens	Ephrin type-A receptor 1	8.2	8.2	8.2	pKd	1
Homo sapiens	Ephrin type-A receptor 2	8.4	8.4	8.4	pKd	1
Homo sapiens	Ephrin type-A receptor 3	9	9	9	pKd	1
Homo sapiens	Ephrin type-A receptor 4	8.7	8.7	8.7	pKd	1
Homo sapiens	Ephrin type-A receptor 5	8.2	8.2	8.2	pKd	1
Homo sapiens	Ephrin type-A receptor 6	9	9	9	pKd	1
Homo sapiens	Ephrin type-A receptor 7	8.6	8.6	8.6	pKd	1
Homo sapiens	Ephrin type-A receptor 8	8.7	8.7	8.7	pKd	1
Homo sapiens	Ephrin type-B receptor 1	7.9	7.9	7.9	pKd	1
Homo sapiens	Ephrin type-B receptor 2	8.4	8.4	8.4	pKd	1
Homo sapiens	Ephrin type-B receptor 3	7.1	7.1	7.1	pKd	1
Homo sapiens	Ephrin type-B receptor 4	7.9	7.9	7.9	pKd	1
Homo sapiens	Epidermal growth factor receptor erbB1	5.5	5.5	5.5	pIC50	3
Homo sapiens	Epidermal growth factor receptor erbB1	6.5	5.8	7.1	pKd	12
Homo sapiens	Epithelial discoidin domain-containing receptor 1	9.7	9.7	9.7	pKd	2
Homo sapiens	Eukaryotic translation initiation factor 2-alpha kinase 1	6	6	6	pKd	1
Homo sapiens	Fibroblast growth factor receptor 1	6.2	6.2	6.2	pKd	1
Homo sapiens	Fibroblast growth factor receptor 2	6.1	6.1	6.1	pKd	1
Homo sapiens	Fibroblast growth factor receptor 3	5.8	5.8	6	pKd	2
Homo sapiens	Focal adhesion kinase 1	6.8	6.8	6.8	pKd	1
Homo sapiens	Hepatocyte growth factor receptor	8.8	7.7	9.4	pIC50	43
Homo sapiens	Hepatocyte growth factor receptor	9	8.9	9.1	pKd	3
Homo sapiens	Homeodomain-interacting protein kinase 1	6.4	6.4	6.4	pKd	1
Homo sapiens	Homeodomain-interacting protein kinase 2	6.7	6.7	6.7	pKd	1
Homo sapiens	Homeodomain-interacting protein kinase 3	7.4	7.4	7.4	pKd	1
Homo sapiens	Homeodomain-interacting protein kinase 4	9.3	9.3	9.3	pKd	1
Homo sapiens	Hormonally up-regulated neu tumor-associated kinase	5.3	5.3	5.3	pKd	1
Homo sapiens	Inhibitor of nuclear factor kappa B kinase alpha subunit	5.2	5.2	5.2	pKd	1
Homo sapiens	Inhibitor of nuclear factor kappa B kinase epsilon subunit	5.9	5.9	5.9	pKd	1
Homo sapiens	Insulin-like growth factor I receptor	6.4	6.4	6.4	pKd	1
Homo sapiens	Insulin receptor	6.7	6.7	6.7	pKd	1
Homo sapiens	Insulin receptor-related protein	7.3	7.3	7.3	pKd	1
Homo sapiens	Interleukin-1 receptor-associated kinase 3	6.4	6.4	6.4	pKd	1
Homo sapiens	Leukocyte tyrosine kinase receptor	7	7	7	pKd	1
Homo sapiens	LIM domain kinase 1	6.2	6.2	6.2	pKd	1
Homo sapiens	LIM domain kinase 2	5.8	5.8	5.8	pKd	1
Homo sapiens	Macrophage colony stimulating factor receptor	8.7	8.7	8.7	pKd	1
Homo sapiens	Macrophage-stimulating protein receptor	8.5	8.5	8.5	pIC50	6
Homo sapiens	Macrophage-stimulating protein receptor	8.5	8.5	8.5	pKd	1
Homo sapiens	MAP kinase-interacting serine/threonine-protein kinase MNK1	7.1	7.1	7.1	pKd	1
Homo sapiens	MAP kinase p38 alpha	6.5	6.5	6.5	pKd	1
Homo sapiens	MAP kinase p38 beta	6.1	6.1	6.1	pKd	1
Homo sapiens	MAP kinase p38 delta	7.3	7.3	7.3	pKd	1
Homo sapiens	MAP kinase p38 gamma	6.4	6.4	6.4	pKd	1
Homo sapiens	MAP kinase signal-integrating kinase 2	8.4	8.4	8.4	pKd	1
Homo sapiens	Maternal embryonic leucine zipper kinase	6.5	6.5	6.5	pKd	1
Homo sapiens	Misshapen-like kinase 1	5.6	5.6	5.6	pKd	1
Homo sapiens	Mitogen-activated protein kinase 15	7.1	7.1	7.1	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase 10	6.3	6.3	6.3	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase 11	6.5	6.5	6.5	pKd	2
Homo sapiens	Mitogen-activated protein kinase kinase kinase 12	7.7	7.7	7.7	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase 13	7.8	7.8	7.8	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase 2	5.7	5.7	5.7	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase 3	5.3	5.3	5.3	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase 4	5.7	5.7	5.7	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase 7	6.3	6.3	6.3	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase 9	6	6	6	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 1	7.4	7.4	7.4	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 2	5.7	5.7	5.7	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 3	7	7	7	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 4	6.7	6.7	6.7	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 5	8.4	8.4	8.4	pKd	1
Homo sapiens	Mixed lineage kinase 7	7.4	7.4	7.4	pKd	1
Homo sapiens	Muscle, skeletal receptor tyrosine protein kinase	7.9	7.9	7.9	pKd	1
Homo sapiens	Myosin IIIA	5.6	5.6	5.6	pKd	1
Homo sapiens	Myosin-IIIB	6.2	6.2	6.2	pKd	1
Homo sapiens	Myosin light chain kinase	5.5	5.5	5.5	pKd	1
Homo sapiens	myosin light chain kinase 2	5.6	5.6	5.6	pKd	1
Homo sapiens	Nerve growth factor receptor Trk-A	8.3	8.3	8.3	pKd	1
Homo sapiens	Neurotrophic tyrosine kinase receptor type 2	8.4	8.4	8.4	pKd	1
Homo sapiens	Non-receptor tyrosine-protein kinase TNK1	7.7	7.7	7.7	pKd	1
Homo sapiens	NT-3 growth factor receptor	8.5	8.5	8.5	pKd	1
Homo sapiens	PITSLRE serine/threonine-protein kinase CDC2L1	6.2	6.2	6.2	pKd	1
Homo sapiens	PITSLRE serine/threonine-protein kinase CDC2L2	6.3	6.3	6.3	pKd	1
Homo sapiens	Platelet-derived growth factor receptor alpha	8.4	8.2	8.4	pIC50	3
Homo sapiens	Platelet-derived growth factor receptor alpha	8.4	8.4	8.4	pKd	1
Homo sapiens	Platelet-derived growth factor receptor beta	8	8	8	pIC50	2
Homo sapiens	Platelet-derived growth factor receptor beta	9	9	9	pKd	1
Homo sapiens	Protein kinase C nu	5.1	5.1	5.1	pKd	1
Homo sapiens	Protein tyrosine kinase 2 beta	7.9	7.9	7.9	pKd	1
Homo sapiens	Proto-oncogene tyrosine-protein kinase MER	9.6	9.6	9.6	pKd	1
Homo sapiens	Proto-oncogene tyrosine-protein kinase ROS	8	8	8	pIC50	1
Homo sapiens	Proto-oncogene tyrosine-protein kinase ROS	7.9	7.9	7.9	pKd	1
Homo sapiens	Proto-oncogene tyrosine-protein kinase ROS	7.8	7.7	8.1	pKi	3
Homo sapiens	Receptor-interacting serine/threonine-protein kinase 1	6.1	6.1	6.1	pKd	1
Homo sapiens	Receptor-interacting serine/threonine-protein kinase 4	6.1	6.1	6.1	pKd	1
Homo sapiens	Receptor protein-tyrosine kinase erbB-2	6.2	6.2	6.2	pKd	1
Homo sapiens	Receptor protein-tyrosine kinase erbB-4	6.2	6.2	6.2	pKd	1
Homo sapiens	Receptor tyrosine-protein kinase erbB-3	5.8	5.8	5.8	pKd	1
Homo sapiens	Rho-associated protein kinase 2	5.8	5.8	5.8	pKd	1
Homo sapiens	Ribosomal protein S6 kinase 1	5.9	5.9	5.9	pKd	1
Homo sapiens	Ribosomal protein S6 kinase alpha 2	5.3	5.3	5.3	pKd	2
Homo sapiens	Serine/threonine-protein kinase 10	9.3	9.3	9.3	pKd	1
Homo sapiens	Serine/threonine-protein kinase 2	8.5	8.5	8.5	pKd	1
Homo sapiens	Serine/threonine-protein kinase 25	5.2	5.2	5.2	pKd	1
Homo sapiens	Serine/threonine-protein kinase 33	7	7	7	pKd	1
Homo sapiens	Serine/threonine-protein kinase 35	6.6	6.6	6.6	pKd	1
Homo sapiens	Serine/threonine-protein kinase 36	6.8	6.8	6.8	pKd	1
Homo sapiens	Serine/threonine-protein kinase Aurora-A	5.9	5.8	6.4	pKd	3
Homo sapiens	Serine/threonine-protein kinase Aurora-B	7.4	7.4	7.4	pKd	1
Homo sapiens	Serine/threonine-protein kinase Aurora-C	7.9	7.9	7.9	pKd	1
Homo sapiens	Serine/threonine-protein kinase B-raf	5.5	5.5	5.6	pKd	2
Homo sapiens	Serine/threonine-protein kinase Chk2	7.2	7.2	7.2	pKd	1
Homo sapiens	Serine/threonine-protein kinase MAK	5.8	5.8	5.8	pKd	1
Homo sapiens	Serine/threonine-protein kinase MRCK-A	6.2	6.2	6.2	pKd	1
Homo sapiens	Serine/threonine-protein kinase MRCK beta	6.5	6.5	6.5	pKd	1
Homo sapiens	Serine/threonine-protein kinase MRCK gamma	6.8	6.8	6.8	pKd	1
Homo sapiens	Serine/threonine-protein kinase MST1	6	6	6	pKd	1
Homo sapiens	Serine/threonine-protein kinase NEK9	6.3	6.3	6.3	pKd	1
Homo sapiens	Serine/threonine protein kinase NLK	6.8	6.8	6.8	pKd	1
Homo sapiens	Serine/threonine-protein kinase PAK 3	5.4	5.4	5.4	pKd	1
Homo sapiens	Serine/threonine-protein kinase PCTAIRE-1	6.1	6.1	6.1	pKd	1
Homo sapiens	Serine/threonine-protein kinase PCTAIRE-2	6.8	6.8	6.8	pKd	1
Homo sapiens	Serine/threonine-protein kinase PCTAIRE-3	6.6	6.6	6.6	pKd	1
Homo sapiens	Serine/threonine-protein kinase PFTAIRE-1	7.5	7.5	7.5	pKd	1
Homo sapiens	Serine/threonine-protein kinase PFTAIRE-2	7.5	7.5	7.5	pKd	1
Homo sapiens	Serine/threonine-protein kinase PLK4	8.5	8.5	8.5	pKd	1
Homo sapiens	Serine/threonine-protein kinase RAF	5.4	5.4	5.4	pKd	1
Homo sapiens	Serine/threonine-protein kinase RIPK2	7.7	7.7	7.7	pKd	1
Homo sapiens	Serine/threonine-protein kinase SIK1	6.8	6.8	6.8	pKd	1
Homo sapiens	Serine/threonine-protein kinase SIK2	6.2	6.2	6.2	pKd	1
Homo sapiens	Serine/threonine-protein kinase TAO1	5.4	5.4	5.4	pKd	1
Homo sapiens	Serine/threonine-protein kinase TAO2	5.2	5.2	5.2	pKd	1
Homo sapiens	Serine/threonine-protein kinase TBK1	6.1	6.1	6.1	pKd	1
Homo sapiens	Serine/threonine-protein kinase TNNI3K	6.9	6.9	6.9	pKd	1
Homo sapiens	Serine/threonine-protein kinase ULK3	6.4	6.4	6.4	pKd	1
Homo sapiens	SPS1/STE20-related protein kinase YSK4	8.3	8.3	8.3	pKd	1
Homo sapiens	Stem cell growth factor receptor	8.2	8.2	8.4	pIC50	6
Homo sapiens	Stem cell growth factor receptor	8.5	7.4	8.7	pKd	8
Homo sapiens	TRAF2- and NCK-interacting kinase	6.7	6.7	6.7	pKd	1
Homo sapiens	Tyrosine kinase non-receptor protein 2	6.7	6.7	6.7	pKd	1
Homo sapiens	Tyrosine-protein kinase ABL	7.4	6.5	8.4	pKd	15
Homo sapiens	Tyrosine-protein kinase ABL2	7.6	7.6	7.6	pKd	1
Homo sapiens	Tyrosine-protein kinase BLK	8.3	8.3	8.3	pKd	1
Homo sapiens	Tyrosine-protein kinase BMX	6.7	6.7	6.7	pKd	1
Homo sapiens	Tyrosine-protein kinase BRK	8.4	8.4	8.4	pKd	1
Homo sapiens	Tyrosine-protein kinase BTK	7.1	7.1	7.1	pKd	1
Homo sapiens	Tyrosine-protein kinase CSK	6.9	6.9	6.9	pKd	1
Homo sapiens	Tyrosine-protein kinase FER	7.4	7.4	7.4	pKd	1
Homo sapiens	Tyrosine-protein kinase FES	7	7	7	pKd	1
Homo sapiens	Tyrosine-protein kinase FGR	7.4	7.4	7.4	pKd	1
Homo sapiens	Tyrosine-protein kinase FRK	8.4	8.4	8.4	pKd	1
Homo sapiens	Tyrosine-protein kinase FYN	7.1	7.1	7.1	pKd	1
Homo sapiens	Tyrosine-protein kinase HCK	7.8	7.8	7.8	pKd	1
Homo sapiens	Tyrosine-protein kinase ITK/TSK	7.2	7.2	7.2	pKd	1
Homo sapiens	Tyrosine-protein kinase JAK2	5.8	5.8	5.8	pKd	2
Homo sapiens	Tyrosine-protein kinase LCK	8.2	8.2	8.2	pKd	1
Homo sapiens	Tyrosine-protein kinase Lyn	8	8	8	pKd	1
Homo sapiens	Tyrosine-protein kinase receptor FLT3	8.3	8.3	8.4	pIC50	5
Homo sapiens	Tyrosine-protein kinase receptor FLT3	8.8	8	9.1	pKd	8
Homo sapiens	Tyrosine-protein kinase receptor RET	8.8	8.6	9.2	pKd	4
Homo sapiens	Tyrosine-protein kinase receptor Tie-1	9.1	9.1	9.1	pKd	1
Homo sapiens	Tyrosine-protein kinase receptor TYRO3	8.7	8.7	8.7	pKd	1
Homo sapiens	Tyrosine-protein kinase receptor UFO	8	8	8	pIC50	1
Homo sapiens	Tyrosine-protein kinase receptor UFO	10	10	10	pKd	1
Homo sapiens	Tyrosine-protein kinase SRC	7.7	7.7	7.7	pKd	1
Homo sapiens	Tyrosine-protein kinase Srms	7.8	7.8	7.8	pKd	1
Homo sapiens	Tyrosine-protein kinase SYK	6.6	6.6	6.6	pKd	1
Homo sapiens	Tyrosine-protein kinase TEC	6.5	6.5	6.5	pKd	1
Homo sapiens	Tyrosine-protein kinase TIE-2	9	9	9	pIC50	1
Homo sapiens	Tyrosine-protein kinase TIE-2	8.5	8.5	8.5	pKd	1
Homo sapiens	Tyrosine-protein kinase TXK	6.3	6.3	6.3	pKd	1
Homo sapiens	Tyrosine-protein kinase TYK2	5.3	5.3	5.3	pKd	2
Homo sapiens	Tyrosine-protein kinase YES	7.3	7.3	7.3	pKd	1
Homo sapiens	Vascular endothelial growth factor receptor 1	8.2	8.2	9.1	pIC50	3
Homo sapiens	Vascular endothelial growth factor receptor 1	8.4	8.4	8.4	pKd	1
Homo sapiens	Vascular endothelial growth factor receptor 2	9.1	7.8	9.1	pIC50	14
Homo sapiens	Vascular endothelial growth factor receptor 2	7.9	7.9	7.9	pKd	1
Homo sapiens	Vascular endothelial growth factor receptor 3	8.6	8.6	8.6	pIC50	3
Homo sapiens	Vascular endothelial growth factor receptor 3	8.8	8.8	8.8	pKd	1

Kinase-ligand interaction pattern

• Hydrophobic • Aromatic face-to-face • Aromatic face-to-edge • H-bond donor • H-bond acceptor • Ionic positive • Ionic negative

g.l

III

αC

1 K
617

2 V
618

3 I
619

4 G
620

5 A
621

6 G
622

7 E
623

8 F
624

9 G
625

10 E
626

11 V
627

12 Y
628

13 K
629

14 V
643

15 A
644

16 I
645

17 K
646

18 T
647

19 L
648

20 D
659

•

••

αC

b.l

21 F
660

22 L
661

23 G
662

24 E
663

25 A
664

26 G
665

27 I
666

28 M
667

29 G
668

30 Q
669

31 F
670

32 S
671

33 H
673

34 N
674

35 I
675

36 I
676

37 R
677

38 L
678

39 E
679

40 G
680

•

••

•

hinge

linker

αD

αE

41 V
681

42 M
689

43 I
690

44 I
691

45 T
692

46 E
693

47 Y
694

48 M
695

49 E
696

50 N
697

51 G
698

52 A
699

53 L
700

54 D
701

55 K
702

56 F
703

57 L
704

58 R
705

59 E
706

60 Y
729

•

••

•

αE

c.l

VII

VIII

61 L
730

62 A
731

63 N
732

64 M
733

65 N
734

66 Y
735

67 V
736

68 H
737

69 R
738

70 D
739

71 L
740

72 A
741

73 A
742

74 R
743

75 N
744

76 I
745

77 L
746

78 V
747

79 V
755

80 S
756

•

••

•

DFG

a.l

81 D
757

82 F
758

83 G
759

84 L
760

85 S
761

••

Interaction pattern search

Search KLIFS for kinase-ligand complexes with similar interaction patterns: