KLIFS - the kinase structures database

Version: 3.2
Updated: 29-Oct-2025

Calcium/calmodulin-dependent protein kinase kinase 2, beta

Kinase structure
Kinase:	CAMKK2 (CaMKK2)
Family:	CAMKK
Group:	Other
Species:	HUMAN
IUPHAR/BPS ID:	1957
PDB:	6BQL
sc-PDB:
KIDFamMap:	Search
Release date:	2017-12-13
Chain:	A
Orthosteric ligand:	BI9

Structural information
DFG conformation:	in
αC-helix conformation:	in
G-rich loop angle (distance):	56.7° (17.5Å)
G-rich loop rotation:	52.4°
Quality Score:	9.2
Resolution:	2 Å
Missing Residues:	0
Missing Atoms:	8

Ligand binding mode
Pockets	Subpockets	Waters
front		Cluster I2	Ligand Yes	Protein Yes

Pocket alignment
Uniprot sequence:	DEIGKGSYGVVKLYAMKVLQVYQEIAILKKLDPNVVKLVEVYMVFELVNQGPVMEVPTLYLHYQKIIHRDIKPSNLLVIADFGVS
Sequence structure:	DEIGKGSYGVVKLYAMKVLQVYQEIAILKKLDPNVVKLVEVYMVFELVNQGPVMEVPTLYLHYQKIIHRDIKPSNLLVIADFGVS

Ligand affinity
ChEMBL ID:	CHEMBL458997
Bioaffinities:	112 records for 97 kinases

Species	Kinase (ChEMBL naming)	Median	Min	Max	Type	Records
Homo sapiens	3-phosphoinositide dependent protein kinase-1	5.7	5.7	5.7	pKi	1
Homo sapiens	ALK tyrosine kinase receptor	7.3	7.3	8.3	pIC50	2
Homo sapiens	ALK tyrosine kinase receptor	7.6	7.6	7.6	pKi	1
Homo sapiens	AMP-activated protein kinase, alpha-1 subunit	6.1	6.1	6.1	pKi	1
Homo sapiens	BR serine/threonine-protein kinase 1	6.1	6.1	6.1	pKi	1
Homo sapiens	CaM kinase II beta	5.2	5.2	5.2	pKi	1
Homo sapiens	CaM kinase II delta	6.5	6.5	6.5	pKi	1
Homo sapiens	CaM kinase II gamma	6.1	6.1	6.1	pKi	1
Homo sapiens	CaM-kinase kinase beta	8.9	8.9	8.9	pKi	1
Homo sapiens	Casein kinase I delta	5.2	5.2	5.2	pKi	1
Homo sapiens	Casein kinase II alpha	7	7	7	pKi	1
Homo sapiens	Cell division cycle 7-related protein kinase	6.5	6.5	6.5	pKi	1
Homo sapiens	cGMP-dependent protein kinase 1 beta	5.9	5.9	5.9	pKi	1
Homo sapiens	c-Jun N-terminal kinase 1	8.1	8.1	8.1	pKi	1
Homo sapiens	c-Jun N-terminal kinase 2	7.4	7.4	7.4	pIC50	1
Homo sapiens	c-Jun N-terminal kinase 2	7.5	7.5	7.5	pKi	1
Homo sapiens	c-Jun N-terminal kinase 3	7.9	7.9	7.9	pIC50	1
Homo sapiens	c-Jun N-terminal kinase 3	8.5	8.5	8.5	pKi	1
Homo sapiens	Cyclin-dependent kinase 1	6.1	6.1	6.1	pKi	1
Homo sapiens	Cyclin-dependent kinase 2	5.7	5.7	5.7	pKi	1
Homo sapiens	Cyclin-dependent kinase 5	5.5	5.5	5.5	pKi	1
Homo sapiens	Cyclin-dependent kinase 7	6.4	6.4	6.4	pKi	1
Homo sapiens	Death-associated protein kinase 3	7	7	7	pKi	1
Homo sapiens	Dual specificity mitogen-activated protein kinase kinase 2	5.5	5.5	5.5	pKi	1
Homo sapiens	Dual specificity protein kinase CLK2	6.3	6.3	6.3	pKi	1
Homo sapiens	Dual specificity protein kinase CLK4	7.8	7.8	7.8	pKi	1
Homo sapiens	Dual-specificity tyrosine-phosphorylation regulated kinase 1A	6.4	6.4	6.4	pKi	1
Homo sapiens	Dual specificity tyrosine-phosphorylation-regulated kinase 1B	6.1	6.1	6.1	pKi	1
Homo sapiens	Dual-specificity tyrosine-phosphorylation regulated kinase 3	6.1	6.1	6.1	pKi	1
Homo sapiens	Dual specificity tyrosine-phosphorylation-regulated kinase 4	6.3	6.3	6.3	pKi	1
Homo sapiens	Epidermal growth factor receptor erbB1	7.5	7.5	7.5	pKi	1
Homo sapiens	Fibroblast growth factor receptor 1	6.2	6.2	6.2	pKi	1
Homo sapiens	Fibroblast growth factor receptor 3	6.3	6.3	6.3	pKi	1
Homo sapiens	Focal adhesion kinase 1	8.2	8.2	8.3	pIC50	6
Homo sapiens	Focal adhesion kinase 1	8.8	8.8	8.8	pKi	1
Homo sapiens	Hepatocyte growth factor receptor	6.7	6.7	6.7	pKi	1
Homo sapiens	Homeodomain-interacting protein kinase 2	6.5	6.5	6.5	pKi	1
Homo sapiens	Homeodomain-interacting protein kinase 4	7	7	7	pKi	1
Homo sapiens	Insulin-like growth factor I receptor	8.4	8.4	8.4	pKi	1
Homo sapiens	Insulin receptor	7.4	7.4	7.6	pIC50	2
Homo sapiens	Insulin receptor	8.3	8.3	8.3	pKi	1
Homo sapiens	Interleukin-1 receptor-associated kinase 1	6.3	6.3	6.3	pKi	1
Homo sapiens	Interleukin-1 receptor-associated kinase 4	6.7	6.7	6.7	pKi	1
Homo sapiens	Leucine-rich repeat serine/threonine-protein kinase 2	8.6	8.6	8.6	pKi	1
Homo sapiens	Leukocyte tyrosine kinase receptor	8.1	8.1	8.1	pKi	1
Homo sapiens	LIM domain kinase 1	5.9	5.9	5.9	pKi	1
Homo sapiens	Macrophage colony stimulating factor receptor	5.8	5.8	5.8	pKi	1
Homo sapiens	Macrophage-stimulating protein receptor	6.1	6.1	6.1	pKi	1
Homo sapiens	MAP kinase ERK2	6.6	6.6	6.6	pKi	1
Homo sapiens	MAP kinase p38 alpha	5.9	5.9	5.9	pKi	1
Homo sapiens	MAP kinase signal-integrating kinase 2	5.9	5.9	5.9	pKi	1
Homo sapiens	MAP/microtubule affinity-regulating kinase 4	6.4	6.4	6.4	pKi	1
Homo sapiens	Maternal embryonic leucine zipper kinase	6.5	6.5	6.5	pKi	1
Homo sapiens	Misshapen-like kinase 1	5.3	5.3	5.3	pKi	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase 10	6.5	6.5	6.5	pKi	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 5	5.3	5.3	5.3	pKi	1
Homo sapiens	Phosphorylase kinase gamma subunit 2	6.5	6.5	6.5	pKi	1
Homo sapiens	Platelet-derived growth factor receptor beta	6	6	6	pKi	1
Homo sapiens	Protein kinase C gamma	5.1	5.1	5.1	pKi	1
Homo sapiens	Protein kinase C nu	6.5	6.5	6.5	pKi	1
Homo sapiens	Proto-oncogene tyrosine-protein kinase ROS	8.3	8.3	8.3	pKi	1
Homo sapiens	Receptor protein-tyrosine kinase erbB-2	7.2	7.2	7.2	pKi	1
Homo sapiens	Receptor protein-tyrosine kinase erbB-4	7.1	7.1	7.1	pKi	1
Homo sapiens	Ribosomal protein S6 kinase 1	5.4	5.4	5.4	pKi	1
Homo sapiens	Ribosomal protein S6 kinase alpha 3	6.3	6.3	6.3	pKi	2
Homo sapiens	Serine/threonine-protein kinase 17A	5.4	5.4	5.4	pKi	1
Homo sapiens	Serine/threonine-protein kinase 2	5.8	5.8	5.8	pKi	1
Homo sapiens	Serine/threonine-protein kinase Aurora-A	8.4	8.4	8.4	pIC50	1
Homo sapiens	Serine/threonine-protein kinase Aurora-A	7.4	7.4	7.4	pKi	1
Homo sapiens	Serine/threonine-protein kinase Aurora-B	7.8	7.8	7.8	pKi	1
Homo sapiens	Serine/threonine-protein kinase Chk1	6	6	6	pKi	1
Homo sapiens	Serine/threonine-protein kinase Chk2	6.5	6.5	6.5	pKi	1
Homo sapiens	Serine/threonine-protein kinase c-TAK1	5.8	5.8	5.8	pKi	1
Homo sapiens	Serine/threonine-protein kinase D2	6	6	6	pKi	1
Homo sapiens	Serine/threonine-protein kinase DCLK1	5.2	5.2	5.2	pKi	1
Homo sapiens	Serine/threonine-protein kinase NEK2	5.6	5.6	5.6	pKi	1
Homo sapiens	Serine/threonine-protein kinase Nek4	6.5	6.5	6.5	pKi	1
Homo sapiens	Serine/threonine-protein kinase PAK 4	5.5	5.5	5.5	pKi	1
Homo sapiens	Serine/threonine-protein kinase PIM3	5.9	5.9	5.9	pKi	1
Homo sapiens	Serine/threonine-protein kinase PLK1	6.7	6.7	6.7	pKi	1
Homo sapiens	Serine/threonine-protein kinase PLK3	5.7	5.7	5.7	pKi	1
Homo sapiens	Serine/threonine-protein kinase PLK4	8.4	8.4	8.4	pKi	1
Homo sapiens	Serine/threonine-protein kinase SIK2	6.2	6.2	6.2	pKi	1
Homo sapiens	Serine/threonine-protein kinase SRPK1	6	6	6	pKi	1
Homo sapiens	Serine/threonine-protein kinase TAO1	5.5	5.5	5.5	pKi	1
Homo sapiens	Serine/threonine-protein kinase TBK1	5.9	5.9	5.9	pKi	1
Homo sapiens	Testis-specific serine/threonine-protein kinase 1	5.8	5.8	5.8	pKi	1
Homo sapiens	Tyrosine kinase non-receptor protein 2	7.4	7.4	7.4	pKi	1
Homo sapiens	Tyrosine-protein kinase BRK	7.3	7.3	7.3	pKi	1
Homo sapiens	Tyrosine-protein kinase BTK	6.3	6.3	6.3	pKi	1
Homo sapiens	Tyrosine-protein kinase FER	8	8	8	pKi	1
Homo sapiens	Tyrosine-protein kinase FES	6.9	6.9	6.9	pKi	1
Homo sapiens	Tyrosine-protein kinase FRK	7.7	7.7	7.7	pKi	1
Homo sapiens	Tyrosine-protein kinase HCK	5.8	5.8	5.8	pKi	1
Homo sapiens	Tyrosine-protein kinase ITK/TSK	6.6	6.6	6.6	pKi	1
Homo sapiens	Tyrosine-protein kinase JAK2	6.1	6.1	6.1	pKi	2
Homo sapiens	Tyrosine-protein kinase LCK	5.9	5.9	5.9	pKi	1
Homo sapiens	Tyrosine-protein kinase receptor FLT3	6.1	6.1	6.1	pKi	1
Homo sapiens	Tyrosine-protein kinase receptor RET	6.4	6.4	6.4	pKi	1
Homo sapiens	Tyrosine-protein kinase Srms	6.1	6.1	6.1	pKi	1
Homo sapiens	Tyrosine-protein kinase SYK	5.9	5.9	5.9	pKi	1
Homo sapiens	Vascular endothelial growth factor receptor 1	5.8	5.8	5.8	pKi	1
Homo sapiens	Vascular endothelial growth factor receptor 2	6.3	6.3	6.3	pKi	1

Kinase-ligand interaction pattern

• Hydrophobic • Aromatic face-to-face • Aromatic face-to-edge • H-bond donor • H-bond acceptor • Ionic positive • Ionic negative

g.l

III

αC

1 D
169

2 E
170

3 I
171

4 G
172

5 K
173

6 G
174

7 S
175

8 Y
176

9 G
177

10 V
178

11 V
179

12 K
180

13 L
181

14 Y
191

15 A
192

16 M
193

17 K
194

18 V
195

19 L
196

20 Q
232

•

αC

b.l

21 V
233

22 Y
234

23 Q
235

24 E
236

25 I
237

26 A
238

27 I
239

28 L
240

29 K
241

30 K
242

31 L
243

32 D
244

33 P
246

34 N
247

35 V
248

36 V
249

37 K
250

38 L
251

39 V
252

40 E
253

•

hinge

linker

αD

αE

41 V
254

42 Y
264

43 M
265

44 V
266

45 F
267

46 E
268

47 L
269

48 V
270

49 N
271

50 Q
272

51 G
273

52 P
274

53 V
275

54 M
276

55 E
277

56 V
278

57 P
279

58 T
280

59 L
281

60 Y
302

•

••

•

αE

c.l

VII

VIII

61 L
303

62 H
304

63 Y
305

64 Q
306

65 K
307

66 I
308

67 I
309

68 H
310

69 R
311

70 D
312

71 I
313

72 K
314

73 P
315

74 S
316

75 N
317

76 L
318

77 L
319

78 V
320

79 I
328

80 A
329

•

DFG

a.l

81 D
330

82 F
331

83 G
332

84 V
333

85 S
334

•

Interaction pattern search

Search KLIFS for kinase-ligand complexes with similar interaction patterns: