KLIFS - the kinase structures database

Version: 3.2
Updated: 29-Oct-2025

Receptor-interacting serine-threonine kinase 4

Kinase structure
Kinase:	Ripk4 (ANKRD3)
Family:	RIPK
Group:	TKL
Species:	MOUSE
PDB:	5WNM
sc-PDB:
KIDFamMap:	Search
Release date:	2018-05-09
PubMed:	29706531
Chain:	A
Orthosteric ligand:	VX6

Structural information
DFG conformation:	in
αC-helix conformation:	in
G-rich loop angle (distance):	-
G-rich loop rotation:	-
Quality Score:	7.2
Resolution:	2.6 Å
Missing Residues:	2
Missing Atoms:	36

Ligand binding mode
Pockets	Subpockets
front	FP-I

Pocket alignment
Uniprot sequence:	EKVGSGGFGQVYKLAIKCSELLEEAKKMEMAKRYILPVYGIGLVMEYMETGSLEKLLASHCMSPPLLHLDLKPANILLISDFGLA
Sequence structure:	EKVGS__FGQVYKLAIKCSELLEEAKKMEMAKRYILPVYGIGLVMEYMETGSLEKLLASHCMSPPLLHLNLKPANILLISDFGLA

Ligand affinity
ChEMBL ID:	CHEMBL572878
Bioaffinities:	576 records for 211 kinases

Species	Kinase (ChEMBL naming)	Median	Min	Max	Type	Records
Homo sapiens	3-phosphoinositide dependent protein kinase-1	5.6	5.6	5.6	pKi	1
Homo sapiens	Activin receptor type-1	5.7	5.7	5.7	pKd	2
Homo sapiens	Activin receptor type-1	5.9	5.9	5.9	pKi	1
Homo sapiens	Adaptor-associated kinase	6.5	6.5	6.5	pKd	2
Homo sapiens	ALK tyrosine kinase receptor	5.5	5.5	5.5	pKd	2
Homo sapiens	ALK tyrosine kinase receptor	5.6	5.6	5.6	pKi	1
Homo sapiens	AMP-activated protein kinase, alpha-1 subunit	6	6	6	pKd	2
Homo sapiens	AMP-activated protein kinase, alpha-1 subunit	5.6	5.6	5.6	pKi	1
Homo sapiens	AMP-activated protein kinase, alpha-2 subunit	6.5	6.5	6.5	pKd	2
Homo sapiens	Ankyrin repeat and protein kinase domain-containing protein 1	5.3	5.3	5.3	pKd	2
Xenopus laevis	Aurora kinase B-A	7.7	7.7	7.7	pIC50	1
Homo sapiens	BMP-2-inducible protein kinase	7.2	7.2	7.2	pKd	2
Homo sapiens	Bone morphogenetic protein receptor type-1B	6	6	6	pKd	1
Homo sapiens	Bone morphogenetic protein receptor type-2	5.2	5.2	5.2	pKd	2
Homo sapiens	BR serine/threonine-protein kinase 1	5.1	5.1	5.1	pKd	1
Homo sapiens	BR serine/threonine-protein kinase 1	5.2	5.2	5.2	pKi	1
Homo sapiens	CaM kinase I alpha	5.2	5.2	5.2	pKd	1
Homo sapiens	CaM kinase I delta	5.3	5.3	5.3	pKd	2
Homo sapiens	CaM kinase I gamma	5.2	5.2	5.2	pKd	1
Homo sapiens	CaM-kinase kinase alpha	5.3	5.3	5.3	pKd	1
Homo sapiens	Casein kinase II alpha	6.4	6.4	6.4	pKd	1
Homo sapiens	Casein kinase II alpha (prime)	6.4	6.4	6.4	pKd	1
Homo sapiens	Cell division cycle 2-like protein kinase 6	6.7	6.7	6.7	pKi	1
Homo sapiens	c-Jun N-terminal kinase 3	5.6	5.6	5.6	pKd	2
Homo sapiens	c-Jun N-terminal kinase 3	5.7	5.7	5.7	pKi	1
Homo sapiens	Cyclin-dependent kinase-like 2	7.3	7.3	7.3	pKd	1
Homo sapiens	Cyclin-dependent kinase-like 3	6.5	6.5	6.5	pKd	1
Homo sapiens	Cyclin-dependent kinase-like 5	5.6	5.6	5.6	pKd	1
Homo sapiens	Death-associated protein kinase 3	5	5	6.7	pKd	2
Homo sapiens	Discoidin domain-containing receptor 2	6.6	6.6	7.8	pIC50	2
Homo sapiens	Discoidin domain-containing receptor 2	6.6	6.6	6.6	pKd	3
Homo sapiens	Dual specificity mitogen-activated protein kinase kinase 1	5.8	5.8	5.8	pKd	2
Homo sapiens	Dual specificity mitogen-activated protein kinase kinase 1	5.9	5.9	5.9	pKi	1
Homo sapiens	Dual specificity mitogen-activated protein kinase kinase 2	5.7	5.7	5.7	pKd	2
Homo sapiens	Dual specificity mitogen-activated protein kinase kinase 2	5.7	5.7	5.7	pKi	1
Homo sapiens	Dual specificity mitogen-activated protein kinase kinase 5	6.6	6.6	6.6	pKd	1
Homo sapiens	Dual specificity mitogen-activated protein kinase kinase 6	5.6	5.6	5.6	pKd	2
Homo sapiens	Dual specificity protein kinase TTK	5.1	5.1	5.1	pKd	1
Homo sapiens	Dual specificity testis-specific protein kinase 1	5.3	5.3	5.3	pKd	2
Homo sapiens	Ephrin type-A receptor 1	6	6	6	pKd	2
Homo sapiens	Ephrin type-A receptor 2	6.1	6.1	6.1	pKd	2
Homo sapiens	Ephrin type-A receptor 2	6.2	6.2	6.2	pKi	1
Homo sapiens	Ephrin type-A receptor 3	5.8	5.8	5.8	pKd	2
Homo sapiens	Ephrin type-A receptor 4	5.4	5.4	5.4	pKd	2
Homo sapiens	Ephrin type-A receptor 6	5.3	5.3	5.3	pKd	1
Homo sapiens	Ephrin type-A receptor 8	6.3	6.3	6.3	pKd	2
Homo sapiens	Ephrin type-B receptor 1	5.7	5.7	5.7	pKd	2
Homo sapiens	Ephrin type-B receptor 2	5.3	5.3	5.3	pKd	1
Homo sapiens	Ephrin type-B receptor 4	5.8	5.8	5.8	pKd	2
Homo sapiens	Epidermal growth factor receptor erbB1	5.8	5.8	5.8	pKi	1
Homo sapiens	Epithelial discoidin domain-containing receptor 1	7.6	7.6	7.6	pKd	2
Homo sapiens	Eukaryotic translation initiation factor 2-alpha kinase 4	5.7	5.7	5.7	pKd	2
Homo sapiens	Fibroblast growth factor receptor 1	6.3	6.3	6.3	pKd	2
Homo sapiens	Fibroblast growth factor receptor 1	6.1	6.1	6.1	pKi	1
Homo sapiens	Fibroblast growth factor receptor 2	6.2	6.2	6.2	pKd	2
Homo sapiens	Fibroblast growth factor receptor 3	6.1	6.1	6.1	pKd	4
Homo sapiens	Fibroblast growth factor receptor 3	6.2	6.2	6.2	pKi	1
Homo sapiens	Fibroblast growth factor receptor 4	5.1	5.1	5.1	pKd	2
Homo sapiens	G protein-coupled receptor kinase 4	6.4	6.4	6.4	pKd	1
Homo sapiens	Hepatocyte growth factor receptor	6.2	6.2	6.4	pKd	4
Homo sapiens	Hormonally up-regulated neu tumor-associated kinase	5	5	5	pKd	1
Homo sapiens	Inhibitor of nuclear factor kappa B kinase epsilon subunit	5.6	5.6	5.6	pKd	2
Homo sapiens	Insulin-like growth factor I receptor	6.3	6.3	6.3	pIC50	1
Homo sapiens	Insulin-like growth factor I receptor	6.1	6.1	6.1	pKd	2
Homo sapiens	Insulin receptor	6.2	6.2	6.3	pKd	3
Homo sapiens	Insulin receptor	6	6	6	pKi	1
Homo sapiens	Insulin receptor-related protein	6.5	6.5	6.6	pKd	3
Homo sapiens	Interleukin-1 receptor-associated kinase 3	5.3	5.3	5.3	pKd	1
Homo sapiens	Interleukin-1 receptor-associated kinase 4	5.4	5.4	5.4	pKd	1
Homo sapiens	Interleukin-1 receptor-associated kinase 4	5.6	5.6	5.6	pKi	1
Homo sapiens	Leucine-rich repeat serine/threonine-protein kinase 2	6.2	6.2	6.2	pKi	1
Homo sapiens	Leukocyte tyrosine kinase receptor	5.1	5.1	5.1	pKd	2
Homo sapiens	Leukocyte tyrosine kinase receptor	6.3	6.3	6.3	pKi	1
Homo sapiens	LIM domain kinase 1	5.6	5.6	5.6	pKd	2
Homo sapiens	LIM domain kinase 1	6.1	6.1	6.1	pKi	1
Homo sapiens	LIM domain kinase 2	5.6	5.6	5.6	pKd	2
Homo sapiens	Macrophage colony stimulating factor receptor	5.8	5.8	5.8	pKd	2
Homo sapiens	Macrophage-stimulating protein receptor	5.9	5.9	5.9	pKd	1
Homo sapiens	Macrophage-stimulating protein receptor	6.1	6.1	6.1	pKi	1
Homo sapiens	MAP kinase-activated protein kinase 2	5.4	5.4	5.4	pKd	1
Homo sapiens	MAP kinase signal-integrating kinase 2	5.3	5.3	5.3	pKd	1
Homo sapiens	MAP/microtubule affinity-regulating kinase 2	5.9	5.9	5.9	pKd	2
Homo sapiens	MAP/microtubule affinity-regulating kinase 2	6	6	6	pKi	1
Homo sapiens	MAP/microtubule affinity-regulating kinase 4	5.3	5.3	5.3	pKd	2
Homo sapiens	MAP/microtubule affinity-regulating kinase 4	6	6	6	pKi	1
Homo sapiens	Maternal embryonic leucine zipper kinase	6.4	6.4	6.4	pIC50	1
Homo sapiens	Maternal embryonic leucine zipper kinase	6.6	6.6	6.6	pKd	2
Homo sapiens	Maternal embryonic leucine zipper kinase	7.4	7.4	7.4	pKi	1
Homo sapiens	Misshapen-like kinase 1	6	6	6	pKd	1
Homo sapiens	Misshapen-like kinase 1	5.2	5.2	5.2	pKi	1
Homo sapiens	Mitogen-activated protein kinase 7	5.2	5.2	5.2	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase 10	5.8	5.8	5.8	pKd	2
Homo sapiens	Mitogen-activated protein kinase kinase kinase 10	6.4	6.4	6.4	pKi	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase 11	6.2	6.2	6.2	pKd	3
Homo sapiens	Mitogen-activated protein kinase kinase kinase 12	6.7	6.7	6.7	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase 13	6.8	6.8	6.8	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase 2	6	6	6	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase 3	5.7	5.7	5.7	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase 4	5.6	5.6	5.6	pKd	2
Homo sapiens	Mitogen-activated protein kinase kinase kinase 7	5.6	5.6	5.6	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase 9	6.4	6.4	6.4	pKd	2
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 1	7.1	7.1	7.1	pKd	2
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 2	5.6	5.6	5.6	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 3	6.5	6.5	6.5	pKd	2
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 4	5.6	5.6	5.6	pKd	2
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 5	7.1	7.1	7.3	pKd	3
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 5	6.8	6.8	6.8	pKi	1
Homo sapiens	Mixed lineage kinase 7	5.6	5.6	5.6	pKd	2
Homo sapiens	Mixed lineage kinase 7	5.1	5.1	5.1	pKi	1
Homo sapiens	Muscle, skeletal receptor tyrosine protein kinase	6.5	6.5	6.5	pKd	2
Homo sapiens	Myosin light chain kinase	7.8	7.8	7.8	pKd	1
Homo sapiens	myosin light chain kinase 2	7.4	7.4	7.4	pKd	2
Homo sapiens	Myosin light chain kinase family member 4	5.7	5.7	5.7	pKd	2
Gallus gallus	Myosin light chain kinase, smooth muscle	7.8	7.8	7.8	pKd	1
Homo sapiens	Nerve growth factor receptor Trk-A	7.4	7.4	8.5	pKd	4
Homo sapiens	Nerve growth factor receptor Trk-A	6.5	6.5	6.5	pKi	1
Homo sapiens	Neurotrophic tyrosine kinase receptor type 2	6.6	6.6	6.6	pKd	2
Homo sapiens	Neurotrophic tyrosine kinase receptor type 2	6.5	6.5	6.5	pKi	1
Homo sapiens	Non-receptor tyrosine-protein kinase TNK1	7.1	7.1	7.1	pKd	3
Homo sapiens	NT-3 growth factor receptor	6.7	6.7	6.7	pKd	2
Homo sapiens	NT-3 growth factor receptor	6.2	6.2	6.2	pKi	1
Homo sapiens	NUAK family SNF1-like kinase 1	6.7	6.7	6.7	pKd	2
Homo sapiens	NUAK family SNF1-like kinase 2	6.3	6.3	6.3	pKd	2
Homo sapiens	Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha	6.9	6.9	6.9	pKd	2
Homo sapiens	Phosphatidylinositol-5-phosphate 4-kinase type-2 beta	6.9	6.9	6.9	pKd	2
Homo sapiens	Phosphorylase kinase gamma subunit 1	5.7	5.7	5.7	pKd	2
Homo sapiens	Phosphorylase kinase gamma subunit 2	5.4	5.4	5.4	pKd	1
Homo sapiens	PITSLRE serine/threonine-protein kinase CDC2L1	6.5	6.5	6.5	pKd	2
Homo sapiens	PITSLRE serine/threonine-protein kinase CDC2L2	6.6	6.6	6.6	pKd	2
Homo sapiens	Platelet-derived growth factor receptor alpha	5.8	5.8	5.8	pKd	2
Homo sapiens	Platelet-derived growth factor receptor beta	6.5	6.5	6.5	pKd	2
Homo sapiens	Platelet-derived growth factor receptor beta	6.4	6.4	6.4	pKi	1
Homo sapiens	Protein kinase C theta	5.3	5.3	5.3	pKd	1
Homo sapiens	Protein kinase C zeta	5.1	5.1	5.1	pKi	1
Homo sapiens	Protein kinase N1	5.8	5.8	5.8	pKd	2
Homo sapiens	Protein kinase N2	5.3	5.3	5.3	pKd	1
Homo sapiens	Protein kinase N2	5.3	5.3	5.3	pKi	1
Homo sapiens	Protein tyrosine kinase 2 beta	5.6	5.6	5.6	pKd	2
Homo sapiens	Proto-oncogene tyrosine-protein kinase MER	5.8	5.8	5.8	pKd	2
Homo sapiens	Proto-oncogene tyrosine-protein kinase ROS	6.2	6.2	6.2	pKd	2
Homo sapiens	Proto-oncogene tyrosine-protein kinase ROS	6.8	6.8	6.8	pKi	1
Homo sapiens	Putative uncharacterized serine/threonine-protein kinase SgK110	6.3	6.3	6.3	pKd	1
Homo sapiens	Receptor-interacting serine/threonine-protein kinase 1	6.7	6.7	6.7	pIC50	1
Homo sapiens	Receptor-interacting serine/threonine-protein kinase 1	7.7	7.7	7.7	pKd	3
Mus musculus	Receptor-interacting serine/threonine-protein kinase 1	6	6	6	pIC50	2
Homo sapiens	Receptor-interacting serine/threonine-protein kinase 4	6.5	6.5	6.5	pKd	1
Homo sapiens	Receptor tyrosine-protein kinase erbB-3	5.2	5.2	5.2	pKd	1
Homo sapiens	Ribosomal protein S6 kinase 1	5.5	5.5	5.5	pKi	1
Homo sapiens	Ribosomal protein S6 kinase alpha 1	5.1	5.1	5.1	pKd	2
Homo sapiens	Ribosomal protein S6 kinase alpha 2	5.7	5.7	5.7	pKd	4
Homo sapiens	Ribosomal protein S6 kinase alpha 3	5.2	5.2	5.2	pKd	4
Homo sapiens	Serine/threonine-protein kinase 10	6.9	6.9	6.9	pKd	2
Homo sapiens	Serine/threonine-protein kinase 11	5.9	5.9	5.9	pKd	2
Homo sapiens	Serine/threonine-protein kinase 16	6.2	6.2	6.2	pKd	2
Homo sapiens	Serine/threonine-protein kinase 17A	5.1	5.1	5.1	pKd	1
Homo sapiens	Serine/threonine-protein kinase 2	7.1	7.1	7.1	pKd	2
Homo sapiens	Serine/threonine-protein kinase 2	6.3	6.3	6.3	pKi	1
Homo sapiens	Serine/threonine-protein kinase 24	5.4	5.4	5.4	pKd	2
Homo sapiens	Serine/threonine-protein kinase 25	5.3	5.3	5.3	pKd	2
Homo sapiens	Serine/threonine-protein kinase 38-like	5.2	5.2	5.2	pKd	1
Homo sapiens	Serine/threonine-protein kinase Aurora-A	7.7	6.5	9.6	pIC50	20
Homo sapiens	Serine/threonine-protein kinase Aurora-A	8.8	7.8	9.5	pKd	10
Homo sapiens	Serine/threonine-protein kinase Aurora-A	9.2	8.5	9.2	pKi	14
Homo sapiens	Serine/threonine-protein kinase Aurora-B	7.4	6.1	7.8	pIC50	14
Homo sapiens	Serine/threonine-protein kinase Aurora-B	8.1	8.1	8.2	pKd	3
Homo sapiens	Serine/threonine-protein kinase Aurora-B	7.8	7.8	8.7	pKi	13
Homo sapiens	Serine/threonine-protein kinase Aurora-C	8.3	7.2	8.3	pIC50	3
Homo sapiens	Serine/threonine-protein kinase Aurora-C	8.2	8.2	8.2	pKd	3
Homo sapiens	Serine/threonine-protein kinase Aurora-C	8.3	8.3	8.3	pKi	10
Homo sapiens	Serine/threonine-protein kinase Chk1	5.1	5.1	5.1	pKd	1
Homo sapiens	Serine/threonine-protein kinase c-TAK1	5.6	5.6	5.6	pKd	2
Homo sapiens	Serine/threonine-protein kinase c-TAK1	5.4	5.4	5.4	pKi	1
Homo sapiens	Serine/threonine-protein kinase D2	5.3	5.3	5.3	pKi	1
Homo sapiens	Serine/threonine-protein kinase DCLK3	5.4	5.4	5.4	pKd	2
Homo sapiens	Serine/threonine-protein kinase GAK	5.5	5.5	5.5	pKd	2
Homo sapiens	Serine/threonine-protein kinase LATS1	5.3	5.3	5.3	pKd	2
Homo sapiens	Serine/threonine-protein kinase LATS2	5.2	5.2	5.2	pKd	1
Homo sapiens	Serine/threonine-protein kinase MARK1	5.3	5.3	5.3	pKd	2
Homo sapiens	Serine/threonine-protein kinase MST1	6.7	6.7	6.7	pKd	2
Homo sapiens	Serine/threonine-protein kinase MST2	6.3	6.3	6.3	pKd	2
Homo sapiens	Serine/threonine-protein kinase MST4	5.5	5.5	5.5	pKd	1
Homo sapiens	Serine/threonine-protein kinase Nek5	5.4	5.4	5.4	pKd	2
Homo sapiens	Serine/threonine-protein kinase NEK7	5.1	5.1	5.1	pKd	1
Homo sapiens	Serine/threonine-protein kinase PAK 1	5.4	5.4	5.4	pKd	2
Homo sapiens	Serine/threonine-protein kinase PAK 1	5.1	5.1	5.1	pKi	1
Homo sapiens	Serine/threonine-protein kinase PAK 2	5.2	5.2	5.2	pKd	2
Homo sapiens	Serine/threonine-protein kinase PAK 3	5.5	5.5	5.5	pKd	2
Homo sapiens	Serine/threonine-protein kinase PAK 4	5.4	5.4	5.4	pKd	2
Homo sapiens	Serine/threonine-protein kinase PAK 4	5.4	5.4	5.4	pKi	1
Homo sapiens	Serine/threonine-protein kinase PAK6	5	5	5	pKd	1
Homo sapiens	Serine/threonine-protein kinase PAK7	5.5	5.5	5.5	pKd	2
Homo sapiens	Serine/threonine-protein kinase PCTAIRE-1	6.6	6.6	6.6	pKd	2
Homo sapiens	Serine/threonine-protein kinase PCTAIRE-2	5.3	5.3	5.3	pKd	1
Mycobacterium tuberculosis	Serine/threonine-protein kinase pknB	5.7	5.7	5.7	pKd	1
Homo sapiens	Serine/threonine-protein kinase PLK4	8	8	8.1	pKd	3
Homo sapiens	Serine/threonine-protein kinase PLK4	8	7.6	8	pKi	3
Homo sapiens	Serine/threonine-protein kinase PRP4 homolog	5.6	5.6	5.6	pKd	1
Homo sapiens	Serine/threonine-protein kinase receptor R3	5	5	5	pKd	1
Homo sapiens	Serine/threonine-protein kinase RIO1	6.4	6.4	6.4	pKd	2
Homo sapiens	Serine/threonine-protein kinase RIO2	6.6	6.6	6.6	pKd	1
Homo sapiens	Serine/threonine-protein kinase RIO3	6.2	6.2	6.2	pKd	2
Homo sapiens	Serine/threonine-protein kinase SIK1	5.6	5.6	5.6	pKd	2
Homo sapiens	Serine/threonine-protein kinase SIK2	5.8	5.8	5.8	pKd	2
Homo sapiens	Serine/threonine-protein kinase SIK2	6	6	6	pKi	1
Homo sapiens	Serine/threonine-protein kinase SRPK1	7.1	7.1	7.1	pKd	2
Homo sapiens	Serine/threonine-protein kinase SRPK2	6.2	6.2	6.2	pKd	2
Homo sapiens	Serine/threonine-protein kinase SRPK3	6.6	6.6	6.6	pKd	1
Homo sapiens	Serine/threonine-protein kinase TAO1	6.5	6.5	6.5	pKd	1
Homo sapiens	Serine/threonine-protein kinase TAO1	7.1	7.1	7.1	pKi	1
Homo sapiens	Serine/threonine-protein kinase TAO2	6.4	6.4	6.4	pKd	1
Homo sapiens	Serine/threonine-protein kinase TAO3	7.1	7.1	7.1	pKd	1
Homo sapiens	Serine/threonine-protein kinase TBK1	6.3	6.3	6.3	pKd	1
Homo sapiens	Serine/threonine-protein kinase tousled-like 1	5.4	5.4	5.4	pKd	1
Homo sapiens	Serine/threonine-protein kinase ULK3	6.5	6.5	6.5	pKd	1
Homo sapiens	Serine/threonine-protein kinase WEE1	5.5	5.5	5.5	pKd	2
Homo sapiens	SPS1/STE20-related protein kinase YSK4	7.3	7.3	7.3	pKd	1
Homo sapiens	Stem cell growth factor receptor	6.5	5.6	6.6	pKd	15
Homo sapiens	TGF-beta receptor type I	5.1	5.1	5.1	pKd	1
Homo sapiens	TRAF2- and NCK-interacting kinase	5.8	5.8	5.8	pKd	2
Homo sapiens	Tyrosine kinase non-receptor protein 2	5.4	5.4	5.4	pKd	2
Homo sapiens	Tyrosine-protein kinase ABL	8	5.1	8.5	pKd	22
Homo sapiens	Tyrosine-protein kinase ABL	7.5	7.4	7.8	pKi	3
Mus musculus	Tyrosine-protein kinase ABL	5.3	5.3	5.3	pIC50	1
Homo sapiens	Tyrosine-protein kinase ABL2	8.4	8.4	8.4	pKd	3
Homo sapiens	Tyrosine-protein kinase BLK	5.7	5.7	5.7	pKd	2
Homo sapiens	Tyrosine-protein kinase BLK	7.5	7.5	7.5	pKi	1
Homo sapiens	Tyrosine-protein kinase BMX	5.6	5.6	5.6	pKd	2
Homo sapiens	Tyrosine-protein kinase BTK	5.4	5.4	5.4	pKd	2
Homo sapiens	Tyrosine-protein kinase BTK	5.8	5.8	5.8	pKi	1
Homo sapiens	Tyrosine-protein kinase CSK	5.3	5.3	5.3	pKd	2
Homo sapiens	Tyrosine-protein kinase FER	5.4	5.4	5.4	pKd	1
Homo sapiens	Tyrosine-protein kinase FES	5.1	5.1	5.1	pKd	1
Homo sapiens	Tyrosine-protein kinase FGR	6.1	6.1	6.1	pKd	2
Homo sapiens	Tyrosine-protein kinase FRK	5.4	5.4	5.4	pKd	2
Homo sapiens	Tyrosine-protein kinase FYN	6.3	6.3	6.3	pKd	2
Homo sapiens	Tyrosine-protein kinase FYN	5.5	5.5	5.5	pKi	1
Homo sapiens	Tyrosine-protein kinase HCK	5.2	5.2	5.2	pKd	1
Homo sapiens	Tyrosine-protein kinase ITK/TSK	6.5	6.5	6.5	pKd	2
Homo sapiens	Tyrosine-protein kinase ITK/TSK	7.2	7.2	7.2	pKi	1
Homo sapiens	Tyrosine-protein kinase JAK1	5.2	5.2	5.2	pKd	2
Homo sapiens	Tyrosine-protein kinase JAK2	6.7	6.7	6.7	pKd	6
Homo sapiens	Tyrosine-protein kinase JAK2	6.6	6.6	6.6	pKi	2
Homo sapiens	Tyrosine-protein kinase JAK3	6.2	6.2	6.2	pKd	4
Homo sapiens	Tyrosine-protein kinase LCK	7.2	7.2	7.2	pKd	2
Homo sapiens	Tyrosine-protein kinase LCK	6.9	6.9	7.1	pKi	2
Homo sapiens	Tyrosine-protein kinase Lyn	6.2	6.2	6.2	pKd	2
Homo sapiens	Tyrosine-protein kinase Lyn	6.6	6.6	6.6	pKi	1
Homo sapiens	Tyrosine-protein kinase receptor FLT3	7.5	7.5	7.5	pIC50	1
Homo sapiens	Tyrosine-protein kinase receptor FLT3	7.9	6.9	8.4	pKd	13
Homo sapiens	Tyrosine-protein kinase receptor FLT3	7.5	6.4	7.5	pKi	5
Homo sapiens	Tyrosine-protein kinase receptor RET	7.6	7.6	8.1	pKd	7
Homo sapiens	Tyrosine-protein kinase receptor RET	7.9	7.9	7.9	pKi	1
Homo sapiens	Tyrosine-protein kinase receptor Tie-1	6.6	6.6	6.6	pKd	2
Homo sapiens	Tyrosine-protein kinase receptor TYRO3	5.9	5.9	5.9	pKi	1
Homo sapiens	Tyrosine-protein kinase receptor UFO	6.7	6.7	6.7	pKd	2
Homo sapiens	Tyrosine-protein kinase receptor UFO	6.7	6.7	6.7	pKi	1
Gallus gallus	Tyrosine-protein kinase SRC	6.5	6.5	6.5	pIC50	1
Homo sapiens	Tyrosine-protein kinase SRC	6.8	6.8	6.8	pKd	2
Homo sapiens	Tyrosine-protein kinase SRC	6.8	6.8	6.8	pKi	1
Homo sapiens	Tyrosine-protein kinase SYK	5.8	5.8	5.8	pKd	2
Homo sapiens	Tyrosine-protein kinase TEC	5.1	5.1	5.1	pKd	1
Homo sapiens	Tyrosine-protein kinase TIE-2	6.4	6.4	6.4	pKd	2
Homo sapiens	Tyrosine-protein kinase TXK	5.6	5.6	5.6	pKd	2
Homo sapiens	Tyrosine-protein kinase TYK2	5.2	5.2	6.3	pKd	4
Homo sapiens	Tyrosine-protein kinase TYK2	6.7	6.7	6.7	pKi	2
Homo sapiens	Tyrosine-protein kinase YES	6.3	6.3	6.3	pKd	2
Homo sapiens	Vascular endothelial growth factor receptor 1	7	7	7	pKd	2
Homo sapiens	Vascular endothelial growth factor receptor 1	5.9	5.9	5.9	pKi	1
Homo sapiens	Vascular endothelial growth factor receptor 2	5.7	5.7	5.7	pKd	2
Homo sapiens	Vascular endothelial growth factor receptor 2	6.5	6.5	6.5	pKi	1
Homo sapiens	Vascular endothelial growth factor receptor 3	5.7	5.7	5.7	pKd	2
Homo sapiens	Vascular endothelial growth factor receptor 3	5.8	5.8	5.8	pKi	1
Homo sapiens	Wee1-like protein kinase 2	5.4	5.4	5.4	pKd	1

Kinase-ligand interaction pattern

• Hydrophobic • Aromatic face-to-face • Aromatic face-to-edge • H-bond donor • H-bond acceptor • Ionic positive • Ionic negative

g.l

III

αC

1 E
26

2 K
27

3 V
28

4 G
29

5 S
30

6 _
_

7 _
_

8 F
33

9 G
34

10 Q
35

11 V
36

12 Y
37

13 K
38

14 L
48

15 A
49

16 I
50

17 K
51

18 C
52

19 S
53

20 E
65

•

αC

b.l

21 L
66

22 L
67

23 E
68

24 E
69

25 A
70

26 K
71

27 K
72

28 M
73

29 E
74

30 M
75

31 A
76

32 K
77

33 R
79

34 Y
80

35 I
81

36 L
82

37 P
83

38 V
84

39 Y
85

40 G
86

•

hinge

linker

αD

αE

41 I
87

42 G
93

43 L
94

44 V
95

45 M
96

46 E
97

47 Y
98

48 M
99

49 E
100

50 T
101

51 G
102

52 S
103

53 L
104

54 E
105

55 K
106

56 L
107

57 L
108

58 A
109

59 S
110

60 H
133

•

••

•

αE

c.l

VII

VIII

61 C
134

62 M
135

63 S
136

64 P
137

65 P
138

66 L
139

67 L
140

68 H
141

69 L
142

70 N
143

71 L
144

72 K
145

73 P
146

74 A
147

75 N
148

76 I
149

77 L
150

78 L
151

79 I
159

80 S
160

•

••

DFG

a.l

81 D
161

82 F
162

83 G
163

84 L
164

85 A
165

•

Interaction pattern search

Search KLIFS for kinase-ligand complexes with similar interaction patterns: