KLIFS - the kinase structures database

Version: 3.2
Updated: 29-Oct-2025

Receptor-interacting serine-threonine kinase 4

Kinase structure
Kinase:	Ripk4 (ANKRD3)
Family:	RIPK
Group:	TKL
Species:	MOUSE
PDB:	5WNK
sc-PDB:
KIDFamMap:	Search
Release date:	2018-05-09
PubMed:	29706531
Chain:	A
Orthosteric ligand:	B6J

Structural information
DFG conformation:	in
αC-helix conformation:	in
G-rich loop angle (distance):	-
G-rich loop rotation:	-
Quality Score:	7.2
Resolution:	3.11 Å
Missing Residues:	2
Missing Atoms:	46

Ligand binding mode
Pockets	Subpockets
front gate	BP-I-B

Pocket alignment
Uniprot sequence:	EKVGSGGFGQVYKLAIKCSELLEEAKKMEMAKRYILPVYGIGLVMEYMETGSLEKLLASHCMSPPLLHLDLKPANILLISDFGLA
Sequence structure:	EKVGS__FGQVYKLAIKCSELLEEAKKMEMAKRYILPVYGIGLVMEYMETGSLEKLLASHCMSPPLLHLNLKPANILLISDFGLA

Ligand affinity
ChEMBL ID:	CHEMBL230011
Bioaffinities:	80 records for 60 kinases

Species	Kinase (ChEMBL naming)	Median	Min	Max	Type	Records
Homo sapiens	Adaptor-associated kinase	6.1	6.1	6.1	pKd	1
Homo sapiens	BMP-2-inducible protein kinase	6.2	6.2	6.2	pKd	1
Homo sapiens	BR serine/threonine-protein kinase 1	6.1	6.1	6.1	pKd	1
Homo sapiens	BR serine/threonine-protein kinase 2	5.9	5.9	5.9	pKd	1
Homo sapiens	Casein kinase I alpha	6.1	6.1	6.1	pKd	1
Homo sapiens	Casein kinase I delta	5.9	5.9	5.9	pKd	1
Homo sapiens	Casein kinase I epsilon	5.9	5.9	5.9	pKd	1
Homo sapiens	Casein kinase I gamma 1	5.2	5.2	5.2	pKd	1
Homo sapiens	Casein kinase I gamma 2	5.6	5.6	5.6	pKd	1
Homo sapiens	Casein kinase II alpha	5.6	5.6	5.6	pKd	1
Homo sapiens	Casein kinase II alpha (prime)	5.8	5.8	5.8	pKd	1
Homo sapiens	Casein kinase I isoform alpha-like	5.7	5.7	5.7	pKd	1
Homo sapiens	Casein kinase I isoform gamma-3	5.1	5.1	5.1	pKd	1
Homo sapiens	Chaperone activity of bc1 complex-like, mitochondrial	7	7	7	pKd	1
Homo sapiens	Dual specificity mitogen-activated protein kinase kinase 3	5.2	5.2	5.2	pKd	1
Homo sapiens	Dual specificity mitogen-activated protein kinase kinase 4	5.4	5.4	5.4	pKd	1
Homo sapiens	Dual specificity protein kinase CLK2	7.4	7.4	7.4	pKd	1
Homo sapiens	Dual specificity protein kinase CLK3	5.2	5.2	5.2	pKd	1
Homo sapiens	Dual specificity protein kinase CLK4	6.9	6.9	6.9	pKd	1
Homo sapiens	Dual specificity protein kinase TTK	5.1	5.1	5.1	pKd	1
Homo sapiens	Dual-specificity tyrosine-phosphorylation regulated kinase 1A	6.2	6.2	6.2	pKd	1
Homo sapiens	Dual specificity tyrosine-phosphorylation-regulated kinase 1B	6.1	6.1	6.1	pKd	1
Homo sapiens	Dual-specificity tyrosine-phosphorylation regulated kinase 2	5.3	5.3	5.3	pKd	1
Homo sapiens	Dual specificty protein kinase CLK1	6.9	6.9	6.9	pKd	1
Homo sapiens	Epidermal growth factor receptor erbB1	5.7	5.1	6.1	pKd	12
Homo sapiens	G protein-coupled receptor kinase 4	5.2	5.2	5.2	pKd	1
Homo sapiens	MAP kinase-interacting serine/threonine-protein kinase MNK1	6.1	6.1	6.1	pKd	1
Homo sapiens	MAP kinase signal-integrating kinase 2	6.1	6.1	6.1	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase 7	5.6	5.6	5.6	pKd	1
Homo sapiens	Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha	5.8	5.8	5.8	pKd	1
Homo sapiens	Phosphatidylinositol-5-phosphate 4-kinase type-2 beta	5.1	5.1	5.1	pKd	1
Homo sapiens	Protein kinase C nu	6.1	6.1	6.1	pKd	1
Homo sapiens	Receptor-interacting serine/threonine-protein kinase 4	7	7	7	pKd	1
Homo sapiens	Receptor protein-tyrosine kinase erbB-2	6.4	6.4	6.4	pKd	1
Homo sapiens	Receptor protein-tyrosine kinase erbB-4	5.7	5.7	5.7	pKd	1
Homo sapiens	Ribosomal protein S6 kinase alpha 1	6.7	6.7	6.7	pKd	2
Homo sapiens	Ribosomal protein S6 kinase alpha 6	6	6	6	pKd	2
Homo sapiens	Serine/threonine-protein kinase 16	6.3	6.3	6.3	pKd	1
Homo sapiens	Serine/threonine-protein kinase B-raf	5.7	5.7	5.7	pKd	2
Homo sapiens	Serine/threonine-protein kinase GAK	5.9	5.9	5.9	pKd	1
Homo sapiens	Serine/threonine-protein kinase LATS1	5.4	5.4	5.4	pKd	1
Homo sapiens	Serine/threonine-protein kinase LATS2	5.8	5.8	5.8	pKd	1
Homo sapiens	Serine/threonine-protein kinase mTOR	6.2	6.2	6.2	pKd	1
Homo sapiens	Serine/threonine-protein kinase Nek1	5.4	5.4	5.4	pKd	1
Homo sapiens	Serine/threonine-protein kinase OSR1	5.6	5.6	5.6	pKd	1
Homo sapiens	Serine/threonine-protein kinase RIO1	5.2	5.2	5.2	pKd	1
Homo sapiens	Serine/threonine-protein kinase RIO2	5.8	5.8	5.8	pKd	1
Homo sapiens	Serine/threonine-protein kinase RIPK2	6.3	6.3	6.3	pKd	1
Homo sapiens	Serine/threonine-protein kinase SRPK1	5.3	5.3	5.3	pKd	1
Homo sapiens	Serine/threonine-protein kinase SRPK3	5	5	5	pKd	1
Homo sapiens	Serine/threonine-protein kinase TAO1	6.3	6.3	6.3	pKd	1
Homo sapiens	Serine/threonine-protein kinase TAO3	5.6	5.6	5.6	pKd	1
Homo sapiens	Serine/threonine-protein kinase TNNI3K	5.9	5.9	5.9	pKd	1
Homo sapiens	SPS1/STE20-related protein kinase YSK4	5.4	5.4	5.4	pKd	1
Homo sapiens	Tyrosine-protein kinase ABL	5.3	5.1	6.1	pKd	5
Homo sapiens	Tyrosine-protein kinase BRK	5.3	5.3	5.3	pKd	1
Homo sapiens	Tyrosine-protein kinase JAK1	6.2	6.2	6.2	pKd	2
Homo sapiens	Tyrosine-protein kinase LCK	5.5	5.5	5.5	pKd	1
Homo sapiens	Tyrosine-protein kinase TYK2	6.1	6.1	6.1	pKd	2
Homo sapiens	Uncharacterized aarF domain-containing protein kinase 4	5.9	5.9	5.9	pKd	1

Kinase-ligand interaction pattern

• Hydrophobic • Aromatic face-to-face • Aromatic face-to-edge • H-bond donor • H-bond acceptor • Ionic positive • Ionic negative

g.l

III

αC

1 E
26

2 K
27

3 V
28

4 G
29

5 S
30

6 _
_

7 _
_

8 F
33

9 G
34

10 Q
35

11 V
36

12 Y
37

13 K
38

14 L
48

15 A
49

16 I
50

17 K
51

18 C
52

19 S
53

20 E
65

•

αC

b.l

21 L
66

22 L
67

23 E
68

24 E
69

25 A
70

26 K
71

27 K
72

28 M
73

29 E
74

30 M
75

31 A
76

32 K
77

33 R
79

34 Y
80

35 I
81

36 L
82

37 P
83

38 V
84

39 Y
85

40 G
86

•

hinge

linker

αD

αE

41 I
87

42 G
93

43 L
94

44 V
95

45 M
96

46 E
97

47 Y
98

48 M
99

49 E
100

50 T
101

51 G
102

52 S
103

53 L
104

54 E
105

55 K
106

56 L
107

57 L
108

58 A
109

59 S
110

60 H
133

•

••

•

αE

c.l

VII

VIII

61 C
134

62 M
135

63 S
136

64 P
137

65 P
138

66 L
139

67 L
140

68 H
141

69 L
142

70 N
143

71 L
144

72 K
145

73 P
146

74 A
147

75 N
148

76 I
149

77 L
150

78 L
151

79 I
159

80 S
160

•

DFG

a.l

81 D
161

82 F
162

83 G
163

84 L
164

85 A
165

••

Interaction pattern search

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