KLIFS - the kinase structures database

Version: 3.2
Updated: 29-Oct-2025

Mitogen-activated protein kinase-activated protein kinase 2

Kinase structure
Kinase:	MAPKAPK2
Family:	MAPKAPK
Group:	CAMK
Species:	HUMAN
IUPHAR/BPS ID:	2094
PDB:	2JBP
sc-PDB:
KIDFamMap:	Search
Release date:	2007-03-20
PubMed:	17132859
Chain:	G
Orthosteric ligand:	P4O

More entries for 2JBP
2JBP	Chain:	A
2JBP	Chain:	B
2JBP	Chain:	C
2JBP	Chain:	D
2JBP	Chain:	E
2JBP	Chain:	F
2JBP	Chain:	H
2JBP	Chain:	I
2JBP	Chain:	J
2JBP	Chain:	K
2JBP	Chain:	L

Structural information
DFG conformation:	in
αC-helix conformation:	in
G-rich loop angle (distance):	46.2° (13.9Å)
G-rich loop rotation:	75.3°
Quality Score:	8
Resolution:	3.31 Å
Missing Residues:	0
Missing Atoms:	0

Ligand binding mode
Pockets	Subpockets	Waters
front		Cluster I4	Ligand No	Protein Yes

Pocket alignment
Uniprot sequence:	QVLGLGINGKVLQFALKMLKARREVELHWRASPHIVRIVDVLIVMECLDGGELFSRIQDYLHSINIAHRDVKPENLLYLTDFGFA
Sequence structure:	QVLGLGINGKVLQFALKMLKARREVELHWRASPHIVRIVDVLIVMECLDGGELFSRIQDYLHSINIAHRDVKPENLLYLTDFGFA

Ligand affinity
ChEMBL ID:	CHEMBL226403
Bioaffinities:	48 records for 41 kinases

Species	Kinase (ChEMBL naming)	Median	Min	Max	Type	Records
Homo sapiens	AMP-activated protein kinase, alpha-1 subunit	6	6	6	pKi	1
Homo sapiens	BR serine/threonine-protein kinase 1	5.6	5.6	5.6	pKi	1
Homo sapiens	CaM kinase II beta	5.2	5.2	5.2	pKi	1
Homo sapiens	CaM kinase II delta	6.1	6.1	6.1	pKi	1
Homo sapiens	CaM kinase II gamma	5.4	5.4	5.4	pKi	1
Homo sapiens	Casein kinase I alpha	6	6	6	pKi	1
Homo sapiens	Casein kinase I delta	6.7	6.7	6.7	pKi	1
Homo sapiens	Casein kinase I gamma 1	6.6	6.6	6.6	pKi	1
Homo sapiens	Casein kinase I gamma 2	6.5	6.5	6.5	pKi	1
Homo sapiens	Casein kinase II alpha	6.2	6.2	6.2	pKi	1
Homo sapiens	c-Jun N-terminal kinase 1	6.5	6.5	6.5	pKi	1
Homo sapiens	c-Jun N-terminal kinase 2	6.4	6.4	6.4	pKi	1
Homo sapiens	Cyclin-dependent kinase 1	5.4	5.4	5.4	pKi	1
Homo sapiens	Death-associated protein kinase 3	7.6	7.6	7.6	pKi	1
Homo sapiens	Dual specificity protein kinase CLK2	5.9	5.9	5.9	pKi	1
Homo sapiens	Dual specificity protein kinase CLK4	7.2	7.2	7.2	pKi	1
Homo sapiens	Dual-specificity tyrosine-phosphorylation regulated kinase 1A	8.4	8.4	8.4	pKi	1
Homo sapiens	Dual-specificity tyrosine-phosphorylation regulated kinase 3	7.1	7.1	7.1	pKi	1
Homo sapiens	Dual specificity tyrosine-phosphorylation-regulated kinase 4	6.5	6.5	6.5	pKi	1
Homo sapiens	Interleukin-1 receptor-associated kinase 1	5.3	5.3	5.3	pKi	1
Homo sapiens	MAP kinase-activated protein kinase 2	7.3	7.3	7.3	pEC50	1
Homo sapiens	MAP kinase-activated protein kinase 2	8.1	8.1	8.1	pIC50	2
Homo sapiens	MAP kinase-activated protein kinase 2	7.5	7.5	7.5	pKi	1
Homo sapiens	MAP kinase-activated protein kinase 3	6.7	6.7	6.7	pIC50	1
Homo sapiens	MAP kinase-activated protein kinase 3	6.8	6.8	6.8	pKi	1
Homo sapiens	MAP kinase-activated protein kinase 5	7	7	7.1	pIC50	2
Homo sapiens	MAP kinase-activated protein kinase 5	7.2	7.2	7.2	pKi	1
Homo sapiens	MAP kinase ERK2	5.5	5.5	5.5	pIC50	1
Homo sapiens	MAP kinase ERK2	6.4	6.4	6.4	pKi	1
Homo sapiens	MAP kinase-interacting serine/threonine-protein kinase MNK1	5.2	5.2	5.2	pIC50	1
Homo sapiens	MAP kinase p38 gamma	5.3	5.3	5.3	pKi	1
Homo sapiens	MAP kinase signal-integrating kinase 2	6	6	6	pKi	1
Homo sapiens	Misshapen-like kinase 1	5.5	5.5	5.5	pKi	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 2	5.7	5.7	5.7	pKi	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 4	6.8	6.8	6.8	pKi	1
Homo sapiens	Protein kinase C nu	6.6	6.6	6.6	pKi	1
Homo sapiens	Ribosomal protein S6 kinase 1	6	6	6	pKi	1
Homo sapiens	Serine/threonine-protein kinase 17A	7.2	7.2	7.2	pKi	1
Homo sapiens	Serine/threonine-protein kinase Aurora-B	6.2	6.2	6.2	pKi	1
Homo sapiens	Serine/threonine-protein kinase Chk2	6.8	6.8	6.8	pKi	1
Homo sapiens	Serine/threonine-protein kinase D2	7.4	7.4	7.4	pKi	1
Homo sapiens	Serine/threonine-protein kinase PIM1	7.1	7.1	7.1	pKi	1
Homo sapiens	Serine/threonine-protein kinase PIM2	6.4	6.4	6.4	pKi	1
Homo sapiens	Serine/threonine-protein kinase PIM3	7.5	7.5	7.5	pKi	1
Homo sapiens	Serine/threonine-protein kinase PLK4	7.2	7.2	7.2	pKi	1
Homo sapiens	Serine/threonine-protein kinase SRPK1	5.4	5.4	5.4	pKi	1

Kinase-ligand interaction pattern

• Hydrophobic • Aromatic face-to-face • Aromatic face-to-edge • H-bond donor • H-bond acceptor • Ionic positive • Ionic negative

g.l

III

αC

1 Q
68

2 V
69

3 L
70

4 G
71

5 L
72

6 G
73

7 I
74

8 N
75

9 G
76

10 K
77

11 V
78

12 L
79

13 Q
80

14 F
90

15 A
91

16 L
92

17 K
93

18 M
94

19 L
95

20 K
100

•

••

αC

b.l

21 A
101

22 R
102

23 R
103

24 E
104

25 V
105

26 E
106

27 L
107

28 H
108

29 W
109

30 R
110

31 A
111

32 S
112

33 P
115

34 H
116

35 I
117

36 V
118

37 R
119

38 I
120

39 V
121

40 D
122

hinge

linker

αD

αE

41 V
123

42 L
135

43 I
136

44 V
137

45 M
138

46 E
139

47 C
140

48 L
141

49 D
142

50 G
143

51 G
144

52 E
145

53 L
146

54 F
147

55 S
148

56 R
149

57 I
150

58 Q
151

59 D
152

60 Y
176

•

••

•

αE

c.l

VII

VIII

61 L
177

62 H
178

63 S
179

64 I
180

65 N
181

66 I
182

67 A
183

68 H
184

69 R
185

70 D
186

71 V
187

72 K
188

73 P
189

74 E
190

75 N
191

76 L
192

77 L
193

78 Y
194

79 L
205

80 T
206

•

DFG

a.l

81 D
207

82 F
208

83 G
209

84 F
210

85 A
211

••

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