KLIFS - the kinase structures database

Version: 3.2
Updated: 01-Nov-2025

V-akt murine thymoma viral oncogene homolog 2

Kinase structure
Kinase:	AKT2
Family:	Akt
Group:	AGC
Species:	HUMAN
IUPHAR/BPS ID:	1480
PDB:	3D0E
sc-PDB:
KIDFamMap:	Search
Release date:	2008-10-21
PubMed:	18800763
Chain:	A
Orthosteric ligand:	G93

More entries for 3D0E
3D0E	Chain:	B

Structural information
DFG conformation:	in
αC-helix conformation:	in
G-rich loop angle (distance):	57.8° (17.6Å)
G-rich loop rotation:	50.3°
Quality Score:	8
Resolution:	2 Å
Missing Residues:	0
Missing Atoms:	0

Ligand binding mode
Pockets	Subpockets	Waters
front gate back	BP-I-B	Cluster I1 I3	Ligand No No	Protein Yes Yes

Pocket alignment
Uniprot sequence:	KLLGKGTFGKVILYAMKILHTVTESRVLQNTRPFLTALKYACFVMEYANGGELFFHLSRYLHSRDVVYRDIKLENLMLITDFGLC
Sequence structure:	KLLGKGTFGKVILYAMKILHTVTESRVLQNTRPFLTALKYACFVMEYANGGELFFHLSRYLHSRDVVYRDIKLENLMLITDFGLC

Ligand affinity
ChEMBL ID:	CHEMBL494089
Bioaffinities:	111 records for 72 kinases

Species	Kinase (ChEMBL naming)	Median	Min	Max	Type	Records
Rattus norvegicus	AMP-activated protein kinase, alpha-2 subunit	7.3	7.3	7.3	pIC50	1
Homo sapiens	CaM kinase I delta	5.6	5.6	5.6	pKd	1
Homo sapiens	CaM kinase IV	6.4	6.4	6.4	pKd	1
Homo sapiens	cAMP-dependent protein kinase alpha-catalytic subunit	7.7	7.7	7.7	pKd	1
Homo sapiens	cAMP-dependent protein kinase beta-1 catalytic subunit	7.9	7.9	7.9	pKd	1
Homo sapiens	cGMP-dependent protein kinase 1 beta	7.5	7.5	7.5	pIC50	1
Homo sapiens	cGMP-dependent protein kinase 1 beta	6.7	6.7	6.7	pKd	1
Homo sapiens	cGMP-dependent protein kinase 2	8.5	8.5	8.5	pKd	1
Homo sapiens	Citron Rho-interacting kinase	6.7	6.7	6.7	pKd	1
Homo sapiens	Death-associated protein kinase 1	5.5	5.5	5.5	pKd	1
Homo sapiens	Death-associated protein kinase 3	7.1	7.1	7.1	pIC50	1
Homo sapiens	Death-associated protein kinase 3	6.3	6.3	6.3	pKd	1
Homo sapiens	Dual specificity protein kinase CLK2	6.1	6.1	6.1	pKd	1
Homo sapiens	Glycogen synthase kinase-3 alpha	5.3	5.3	5.3	pKd	1
Homo sapiens	Glycogen synthase kinase-3 beta	6.9	6.9	6.9	pIC50	1
Homo sapiens	Glycogen synthase kinase-3 beta	7	7	7	pKd	1
Homo sapiens	G protein-coupled receptor kinase 7	6.4	6.4	6.4	pKd	1
Homo sapiens	Homeodomain-interacting protein kinase 1	6.3	6.3	6.3	pKd	1
Homo sapiens	Homeodomain-interacting protein kinase 2	6.4	6.4	6.4	pKd	1
Homo sapiens	Homeodomain-interacting protein kinase 3	6.7	6.7	6.7	pKd	1
Homo sapiens	Interferon-induced, double-stranded RNA-activated protein kinase	5.7	5.7	5.7	pKd	1
Homo sapiens	MAP/microtubule affinity-regulating kinase 2	6.1	6.1	6.1	pKd	1
Homo sapiens	Maternal embryonic leucine zipper kinase	6.1	6.1	6.1	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase 2	6.5	6.5	6.5	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase 3	6.1	6.1	6.1	pKd	1
Homo sapiens	NUAK family SNF1-like kinase 1	6.4	6.4	6.4	pKd	1
Homo sapiens	NUAK family SNF1-like kinase 2	7.1	7.1	7.1	pKd	1
Homo sapiens	Platelet-derived growth factor receptor beta	5.4	5.4	5.4	pKd	1
Homo sapiens	Protein kinase C beta	7.7	7.7	7.7	pIC50	1
Homo sapiens	Protein kinase C delta	7.9	7.9	7.9	pIC50	1
Homo sapiens	Protein kinase C delta	7.3	7.3	7.3	pKd	1
Homo sapiens	Protein kinase C epsilon	7.7	7.7	7.7	pIC50	1
Homo sapiens	Protein kinase C epsilon	8.3	8.3	8.3	pKd	1
Homo sapiens	Protein kinase C eta	8.7	8.7	8.7	pIC50	1
Homo sapiens	Protein kinase C eta	8.6	8.6	8.6	pKd	1
Homo sapiens	Protein kinase C iota	6.6	6.6	6.6	pKd	1
Homo sapiens	Protein kinase C mu	5.5	5.5	5.5	pKd	1
Homo sapiens	Protein kinase C nu	6	6	6	pKd	1
Homo sapiens	Protein kinase C theta	8.7	8.7	8.7	pIC50	1
Homo sapiens	Protein kinase C theta	7.8	7.8	7.8	pKd	1
Homo sapiens	Protein kinase N1	7.5	7.5	7.5	pKd	1
Homo sapiens	Protein kinase N2	5.5	5.5	5.5	pKd	1
Rattus norvegicus	RAC-alpha serine/threonine-protein kinase	5.2	5.2	5.2	pIC50	1
Homo sapiens	Rho-associated protein kinase 1	6.1	6.1	6.1	pIC50	1
Homo sapiens	Rho-associated protein kinase 1	6.6	6.6	6.6	pKd	1
Homo sapiens	Rho-associated protein kinase 2	6.7	6.7	6.7	pKd	1
Homo sapiens	Rhodopsin kinase	6.2	6.2	6.2	pKd	1
Homo sapiens	Ribosomal protein S6 kinase alpha 1	5.9	5.9	5.9	pIC50	2
Homo sapiens	Ribosomal protein S6 kinase alpha 4	7.3	7.3	7.3	pKd	2
Homo sapiens	Ribosomal protein S6 kinase alpha 5	5.1	5.1	5.1	pIC50	2
Homo sapiens	Ribosomal protein S6 kinase alpha 5	6.8	6.8	6.8	pKd	2
Homo sapiens	Serine/threonine-protein kinase 36	5.6	5.6	5.6	pKd	1
Homo sapiens	Serine/threonine-protein kinase AKT	8.7	4.8	9.2	pIC50	7
Homo sapiens	Serine/threonine-protein kinase AKT	8.7	8.7	8.7	pKd	1
Homo sapiens	Serine/threonine-protein kinase AKT	9	9	9	pKi	2
Homo sapiens	Serine/threonine-protein kinase AKT2	7.9	7.9	7.9	pIC50	2
Homo sapiens	Serine/threonine-protein kinase AKT2	8.7	8.7	8.7	pKd	1
Homo sapiens	Serine/threonine-protein kinase AKT2	8.4	8.4	8.4	pKi	2
Homo sapiens	Serine/threonine-protein kinase AKT3	8.1	8.1	8.1	pIC50	2
Homo sapiens	Serine/threonine-protein kinase AKT3	8.5	8.5	8.5	pKd	1
Homo sapiens	Serine/threonine-protein kinase AKT3	7.9	7.9	7.9	pKi	1
Homo sapiens	Serine/threonine-protein kinase Chk2	5.3	5.3	5.3	pKd	1
Homo sapiens	Serine/threonine-protein kinase DCLK1	5.6	5.6	5.6	pKd	1
Homo sapiens	Serine/threonine-protein kinase DCLK3	7	7	7	pKd	1
Homo sapiens	Serine/threonine-protein kinase LATS2	6.9	6.9	6.9	pKd	1
Homo sapiens	Serine/threonine-protein kinase MST4	5.3	5.3	5.3	pKd	1
Homo sapiens	Serine/threonine protein kinase NLK	5.5	5.5	5.5	pKd	1
Homo sapiens	Serine/threonine-protein kinase PAK 1	6.3	6.3	6.3	pKd	1
Homo sapiens	Serine/threonine-protein kinase PAK 2	6.6	6.6	6.6	pKd	1
Homo sapiens	Serine/threonine-protein kinase PAK 4	8	8	8	pIC50	1
Homo sapiens	Serine/threonine-protein kinase PAK 4	7.8	7.8	7.8	pKd	1
Homo sapiens	Serine/threonine-protein kinase PAK6	8.2	8.2	8.2	pIC50	1
Homo sapiens	Serine/threonine-protein kinase PAK6	7.7	7.7	7.7	pKd	1
Homo sapiens	Serine/threonine-protein kinase PAK7	7.3	7.3	7.3	pIC50	1
Homo sapiens	Serine/threonine-protein kinase PAK7	8.1	8.1	8.1	pKd	1
Homo sapiens	Serine/threonine-protein kinase PIM1	6	6	6	pKd	1
Homo sapiens	Serine/threonine-protein kinase PIM2	6.2	6.2	6.2	pKd	1
Homo sapiens	Serine/threonine-protein kinase PIM3	6.1	6.1	6.1	pKd	1
Mycobacterium tuberculosis	Serine/threonine-protein kinase pknB	8.5	8.5	8.5	pKd	1
Homo sapiens	Serine/threonine-protein kinase PRKX	8.3	8.3	8.3	pIC50	1
Homo sapiens	Serine/threonine-protein kinase PRKX	8.1	8.1	8.1	pKd	1
Homo sapiens	Serine/threonine-protein kinase Sgk3	5.7	5.7	5.7	pKd	1
Homo sapiens	Serine/threonine-protein kinase SIK2	5.2	5.2	5.2	pKd	1
Homo sapiens	Serine/threonine-protein kinase TAO1	6.2	6.2	6.2	pKd	1
Homo sapiens	Serine/threonine-protein kinase TAO2	5.9	5.9	5.9	pKd	1
Homo sapiens	Serine/threonine-protein kinase TAO3	5	5	5	pKd	1
Homo sapiens	Serine/threonine-protein kinase ULK1	5.2	5.2	5.2	pKd	1
Homo sapiens	Serine/threonine-protein kinase ULK2	5.6	5.6	5.6	pKd	1
Homo sapiens	SPS1/STE20-related protein kinase YSK4	7.5	7.5	7.5	pKd	1
Homo sapiens	Stem cell growth factor receptor	5.8	5.1	6.9	pKd	7
Homo sapiens	Tyrosine-protein kinase receptor RET	5.9	5.9	5.9	pKd	1

Kinase-ligand interaction pattern

• Hydrophobic • Aromatic face-to-face • Aromatic face-to-edge • H-bond donor • H-bond acceptor • Ionic positive • Ionic negative

g.l

III

αC

1 K
156

2 L
157

3 L
158

4 G
159

5 K
160

6 G
161

7 T
162

8 F
163

9 G
164

10 K
165

11 V
166

12 I
167

13 L
168

14 Y
178

15 A
179

16 M
180

17 K
181

18 I
182

19 L
183

20 H
196

•

αC

b.l

21 T
197

22 V
198

23 T
199

24 E
200

25 S
201

26 R
202

27 V
203

28 L
204

29 Q
205

30 N
206

31 T
207

32 R
208

33 P
210

34 F
211

35 L
212

36 T
213

37 A
214

38 L
215

39 K
216

40 Y
217

•

hinge

linker

αD

αE

41 A
218

42 C
226

43 F
227

44 V
228

45 M
229

46 E
230

47 Y
231

48 A
232

49 N
233

50 G
234

51 G
235

52 E
236

53 L
237

54 F
238

55 F
239

56 H
240

57 L
241

58 S
242

59 R
243

60 Y
265

•

••

•••

αE

c.l

VII

VIII

61 L
266

62 H
267

63 S
268

64 R
269

65 D
270

66 V
271

67 V
272

68 Y
273

69 R
274

70 D
275

71 I
276

72 K
277

73 L
278

74 E
279

75 N
280

76 L
281

77 M
282

78 L
283

79 I
291

80 T
292

•••

•

DFG

a.l

81 D
293

82 F
294

83 G
295

84 L
296

85 C
297

••

Interaction pattern search

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