KLIFS - the kinase structures database

Version: 3.2
Updated: 29-Oct-2025

HCK proto-oncogene, Src family tyrosine kinase

Kinase structure
Kinase:	HCK
Family:	Src
Group:	TK
Species:	HUMAN
IUPHAR/BPS ID:	2032
PDB:	3VS7
sc-PDB:
KIDFamMap:	Search
Release date:	2013-05-01
PubMed:	23596204
Chain:	B
Orthosteric ligand:	KS1

More entries for 3VS7
3VS7	Chain:	A

Structural information
DFG conformation:	in
αC-helix conformation:	out
G-rich loop angle (distance):	55.2° (17.3Å)
G-rich loop rotation:	51.2°
Quality Score:	8
Resolution:	3 Å
Missing Residues:	0
Missing Atoms:	0

Ligand binding mode
Pockets	Subpockets
front gate	BP-I-B

Pocket alignment
Uniprot sequence:	KKLGAGQFGEVWMVAVKTMAFLAEANVMKTLQDKLVKLHAVYIITEFMAKGSLLDFLKSFIEQRNYIHRDLRAANILVIADFGLA
Sequence structure:	KKLGAGQFGEVWMVAVKTMAFLAEANVMKTLQDKLVKLHAVYIITEFMAKGSLLDFLKSFIEQRNYIHRDLRAANILVIADFGLA

Ligand affinity
ChEMBL ID:	CHEMBL1081312
Bioaffinities:	66 records for 54 kinases

Species	Kinase (ChEMBL naming)	Median	Min	Max	Type	Records
Homo sapiens	Activin receptor type-1	7.2	7.2	7.2	pKi	1
Homo sapiens	ALK tyrosine kinase receptor	6.2	6.2	6.2	pKi	1
Homo sapiens	AMP-activated protein kinase, alpha-1 subunit	5.6	5.6	5.6	pKi	1
Homo sapiens	cAMP-dependent protein kinase alpha-catalytic subunit	6.1	6.1	6.1	pKi	1
Homo sapiens	Casein kinase I alpha	7.4	7.4	7.4	pKi	1
Homo sapiens	Cell division protein kinase 8	5.9	5.9	5.9	pKi	1
Homo sapiens	c-Jun N-terminal kinase 1	5.6	5.6	5.6	pKi	1
Homo sapiens	c-Jun N-terminal kinase 2	5.9	5.9	5.9	pKi	1
Homo sapiens	Cyclin-dependent kinase 5	5.9	5.9	5.9	pKi	1
Homo sapiens	Death-associated protein kinase 3	5.3	5.3	5.3	pKi	1
Homo sapiens	DNA-dependent protein kinase	7.2	7.2	7.2	pIC50	1
Homo sapiens	Dual specificity mitogen-activated protein kinase kinase 1	6.4	6.4	6.4	pKi	1
Homo sapiens	Dual specificity mitogen-activated protein kinase kinase 2	6	6	6	pKi	1
Homo sapiens	Dual specificity protein kinase CLK2	6	6	6	pKi	1
Homo sapiens	Dual specificity protein kinase CLK4	6.1	6.1	6.1	pKi	1
Homo sapiens	Ephrin type-B receptor 4	6.7	6.7	6.7	pIC50	1
Homo sapiens	Epidermal growth factor receptor erbB1	6.6	6.6	6.6	pIC50	2
Homo sapiens	Fibroblast growth factor receptor 1	7	7	7	pKi	1
Homo sapiens	Focal adhesion kinase 1	5.9	5.9	5.9	pKi	1
Homo sapiens	Glycogen synthase kinase-3 alpha	5.7	5.7	5.7	pKi	1
Homo sapiens	Inhibitor of nuclear factor kappa B kinase epsilon subunit	5.8	5.8	5.8	pKi	1
Homo sapiens	Insulin-like growth factor I receptor	6.4	6.4	6.4	pKi	1
Homo sapiens	Leucine-rich repeat serine/threonine-protein kinase 2	7.2	7.2	7.2	pKi	1
Homo sapiens	Leukocyte tyrosine kinase receptor	6.8	6.8	6.8	pKi	1
Homo sapiens	LIM domain kinase 1	6.8	6.8	6.8	pKi	1
Homo sapiens	Macrophage colony stimulating factor receptor	6.4	6.4	6.4	pKi	1
Homo sapiens	Macrophage-stimulating protein receptor	6.9	6.9	6.9	pKi	1
Homo sapiens	MAP kinase p38 alpha	5.6	5.6	5.6	pKi	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 2	6.5	6.5	6.5	pKi	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 4	6.2	6.2	6.2	pKi	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 5	5.9	5.9	5.9	pKi	1
Homo sapiens	Platelet-derived growth factor receptor alpha	6.5	6.5	6.5	pKi	1
Homo sapiens	Platelet-derived growth factor receptor beta	6.4	6.4	6.4	pKi	1
Homo sapiens	Protein kinase C nu	6.6	6.6	6.6	pKi	1
Homo sapiens	Protein kinase C theta	6.3	6.3	6.3	pKi	1
Homo sapiens	Rho-associated protein kinase 1	6.7	6.7	6.7	pKi	1
Homo sapiens	Rho-associated protein kinase 2	7.4	7.4	7.4	pKi	1
Homo sapiens	Serine-protein kinase ATR	5.9	5.9	5.9	pIC50	1
Homo sapiens	Serine/threonine-protein kinase c-TAK1	6.1	6.1	6.1	pKi	1
Homo sapiens	Serine/threonine-protein kinase mTOR	8	8	8	pIC50	2
Homo sapiens	Serine/threonine-protein kinase PAK 4	6.2	6.2	6.2	pKi	1
Homo sapiens	Serine/threonine-protein kinase TAO1	5.6	5.6	5.6	pKi	1
Homo sapiens	Tyrosine kinase non-receptor protein 2	6.4	6.4	6.4	pKi	1
Homo sapiens	Tyrosine-protein kinase ABL	7.8	7.8	8.7	pIC50	3
Homo sapiens	Tyrosine-protein kinase ABL	7.8	7.8	7.8	pKi	1
Homo sapiens	Tyrosine-protein kinase BTK	5.8	5.8	5.8	pKi	1
Homo sapiens	Tyrosine-protein kinase FYN	7.1	7.1	7.1	pKi	1
Homo sapiens	Tyrosine-protein kinase HCK	8.1	8.1	8.1	pIC50	2
Homo sapiens	Tyrosine-protein kinase JAK2	6	6	6	pKi	2
Homo sapiens	Tyrosine-protein kinase LCK	6.8	6.8	6.8	pKi	1
Homo sapiens	Tyrosine-protein kinase receptor RET	8.9	8.9	8.9	pKi	1
Homo sapiens	Tyrosine-protein kinase receptor TYRO3	6.1	6.1	6.1	pKi	1
Homo sapiens	Tyrosine-protein kinase SRC	7.9	7.9	7.9	pIC50	3
Homo sapiens	Vascular endothelial growth factor receptor 1	7.2	7.2	7.2	pKi	1
Homo sapiens	Vascular endothelial growth factor receptor 2	7.9	7.4	7.9	pIC50	3
Homo sapiens	Vascular endothelial growth factor receptor 2	8.3	8.3	8.3	pKi	1

Kinase-ligand interaction pattern

• Hydrophobic • Aromatic face-to-face • Aromatic face-to-edge • H-bond donor • H-bond acceptor • Ionic positive • Ionic negative

g.l

III

αC

1 K
271

2 K
272

3 L
273

4 G
274

5 A
275

6 G
276

7 Q
277

8 F
278

9 G
279

10 E
280

11 V
281

12 W
282

13 M
283

14 V
292

15 A
293

16 V
294

17 K
295

18 T
296

19 M
297

20 A
306

•

αC

b.l

21 F
307

22 L
308

23 A
309

24 E
310

25 A
311

26 N
312

27 V
313

28 M
314

29 K
315

30 T
316

31 L
317

32 Q
318

33 D
320

34 K
321

35 L
322

36 V
323

37 K
324

38 L
325

39 H
326

40 A
327

hinge

linker

αD

αE

41 V
328

42 Y
335

43 I
336

44 I
337

45 T
338

46 E
339

47 F
340

48 M
341

49 A
342

50 K
343

51 G
344

52 S
345

53 L
346

54 L
347

55 D
348

56 F
349

57 L
350

58 K
351

59 S
352

60 F
376

•

••

•

αE

c.l

VII

VIII

61 I
377

62 E
378

63 Q
379

64 R
380

65 N
381

66 Y
382

67 I
383

68 H
384

69 R
385

70 D
386

71 L
387

72 R
388

73 A
389

74 A
390

75 N
391

76 I
392

77 L
393

78 V
394

79 I
402

80 A
403

•

DFG

a.l

81 D
404

82 F
405

83 G
406

84 L
407

85 A
408

•

Interaction pattern search

Search KLIFS for kinase-ligand complexes with similar interaction patterns: