KLIFS - the kinase structures database

Version: 3.2
Updated: 29-Oct-2025

Dual specificity tyrosine phosphorylation regulated kinase 1A

Kinase structure
Kinase:	DYRK1A
Family:	DYRK
Group:	CMGC
Species:	HUMAN
IUPHAR/BPS ID:	2009
PDB:	4NCT
sc-PDB:
KIDFamMap:	Search
Release date:	2015-04-01
PubMed:	25945585
Chain:	B
Orthosteric ligand:	2K2

More entries for 4NCT
4NCT	Chain:	A
4NCT	Chain:	C
4NCT	Chain:	D

Structural information
DFG conformation:	in
αC-helix conformation:	in
G-rich loop angle (distance):	57.1° (17.7Å)
G-rich loop rotation:	58.7°
Quality Score:	8
Resolution:	2.6 Å
Missing Residues:	0
Missing Atoms:	0

Ligand binding mode
Pockets	Subpockets	Waters
front	FP-II	Cluster I4	Ligand No	Protein Yes

Pocket alignment
Uniprot sequence:	SLIGKGSFGQVVKVAIKIIQAQIEVRLLELMNYYIVHLKRHCLVFEMLS-YNLYDLLRNATPELSIIHCDLKPENILLIVDFGSS
Sequence structure:	SLIGKGSFGQVVKVAIKIIQAQIEVRLLELMNYYIVHLKRHCLVFEMLS_YNLYDLLRNATPELSIIHCDLKPENILLIVDFGSS

Ligand affinity
ChEMBL ID:	CHEMBL608533
Bioaffinities:	637 records for 197 kinases

Species	Kinase (ChEMBL naming)	Median	Min	Max	Type	Records
Homo sapiens	3-phosphoinositide dependent protein kinase-1	6.7	6.7	7	pKd	3
Homo sapiens	Adaptor-associated kinase	7.3	7.3	7.3	pKd	3
Homo sapiens	ALK tyrosine kinase receptor	6.6	6.6	6.6	pKd	3
Homo sapiens	AMP-activated protein kinase, alpha-1 subunit	6.8	6.8	6.8	pKd	3
Homo sapiens	AMP-activated protein kinase, alpha-2 subunit	6.3	6.3	6.3	pKd	3
Homo sapiens	BMP-2-inducible protein kinase	6.7	6.7	6.7	pKd	3
Homo sapiens	BR serine/threonine-protein kinase 1	5.9	5.9	5.9	pKd	3
Homo sapiens	BR serine/threonine-protein kinase 2	6.2	6.2	6.2	pKd	3
Plasmodium falciparum (isolate 3D7)	Calcium-dependent protein kinase 1	6	6	6	pKd	1
Homo sapiens	CaM kinase I alpha	5.7	5.7	5.7	pKd	3
Homo sapiens	CaM kinase I delta	6.2	6.2	6.2	pKd	3
Homo sapiens	CaM kinase I gamma	5.8	5.8	5.8	pKd	2
Homo sapiens	CaM kinase II alpha	7.7	7.7	7.7	pKd	3
Homo sapiens	CaM kinase II beta	6.7	6.7	6.7	pKd	3
Homo sapiens	CaM kinase II delta	7.4	7.4	7.4	pKd	3
Homo sapiens	CaM kinase II gamma	6.9	5.8	6.9	pKd	4
Homo sapiens	CaM-kinase kinase alpha	6.9	6.9	6.9	pKd	3
Homo sapiens	CaM-kinase kinase beta	7.1	7.1	7.1	pKd	3
Homo sapiens	cAMP-dependent protein kinase alpha-catalytic subunit	6.1	6.1	6.1	pKd	2
Homo sapiens	cAMP-dependent protein kinase beta-1 catalytic subunit	6.6	6.6	6.6	pKd	3
Homo sapiens	Casein kinase II alpha	6.6	6.6	6.6	pKd	2
Homo sapiens	Cell division cycle 2-like protein kinase 6	5.1	5.1	5.1	pKd	3
Homo sapiens	cGMP-dependent protein kinase 1 beta	6.6	6.6	6.6	pKd	3
Homo sapiens	cGMP-dependent protein kinase 2	7.1	7.1	7.1	pKd	3
Homo sapiens	c-Jun N-terminal kinase 1	5.4	5.4	5.4	pKd	3
Homo sapiens	c-Jun N-terminal kinase 2	5.3	5.3	5.3	pKd	1
Homo sapiens	c-Jun N-terminal kinase 3	5.4	5.4	5.4	pKd	3
Homo sapiens	Death-associated protein kinase 1	5.3	5.3	5.3	pKd	3
Homo sapiens	Death-associated protein kinase 2	6.1	6.1	6.1	pKd	3
Homo sapiens	Death-associated protein kinase 3	5.8	5.8	5.8	pKd	3
Homo sapiens	Dual specificity mitogen-activated protein kinase kinase 3	5.3	5.3	5.3	pKd	3
Homo sapiens	Dual specificity mitogen-activated protein kinase kinase 4	5.3	5.3	5.3	pKd	3
Homo sapiens	Dual specificity mitogen-activated protein kinase kinase 6	5.2	5.2	5.2	pKd	1
Homo sapiens	Dual specificity protein kinase CLK2	6.1	6.1	6.1	pKd	3
Homo sapiens	Dual specificity protein kinase CLK3	5.4	5.4	5.4	pKd	1
Homo sapiens	Dual specificity protein kinase CLK4	6.4	6.4	6.4	pKd	3
Homo sapiens	Dual specificity protein kinase TTK	6.5	6.5	6.5	pKd	3
Homo sapiens	Dual-specificity tyrosine-phosphorylation regulated kinase 1A	7	7	7	pKd	2
Homo sapiens	Dual specificity tyrosine-phosphorylation-regulated kinase 1B	6.5	6.5	6.5	pKd	3
Homo sapiens	Dual specificty protein kinase CLK1	6.5	6.5	6.5	pKd	3
Homo sapiens	Epidermal growth factor receptor erbB1	5.8	5.2	8.1	pKd	28
Homo sapiens	Epithelial discoidin domain-containing receptor 1	5.1	5.1	5.1	pKd	3
Homo sapiens	Eukaryotic translation initiation factor 2-alpha kinase 4	7.4	7.4	7.4	pKd	3
Homo sapiens	Fibroblast growth factor receptor 1	5.8	5.8	5.8	pKd	3
Homo sapiens	Fibroblast growth factor receptor 2	5.6	5.6	5.6	pKd	3
Homo sapiens	Fibroblast growth factor receptor 3	5.8	5.8	5.8	pKd	3
Homo sapiens	Glycogen synthase kinase-3 alpha	5.6	5.6	5.6	pKd	3
Homo sapiens	Glycogen synthase kinase-3 beta	5.8	5.8	5.8	pKd	3
Homo sapiens	G protein-coupled receptor kinase 4	7	7	7	pKd	2
Homo sapiens	G protein-coupled receptor kinase 7	7.3	7.3	7.3	pKd	2
Homo sapiens	Hepatocyte growth factor receptor	6.2	5.7	6.3	pKd	7
Homo sapiens	Homeodomain-interacting protein kinase 2	5.5	5.5	5.5	pKd	2
Homo sapiens	Homeodomain-interacting protein kinase 3	5.6	5.6	5.6	pKd	2
Homo sapiens	Homeodomain-interacting protein kinase 4	6	6	6	pKd	2
Homo sapiens	Hormonally up-regulated neu tumor-associated kinase	6.6	6.6	6.6	pKd	2
Homo sapiens	Inhibitor of nuclear factor kappa B kinase alpha subunit	5.4	5.4	5.4	pKd	2
Homo sapiens	Inhibitor of nuclear factor kappa B kinase beta subunit	5.2	5.2	5.2	pKd	1
Homo sapiens	Inhibitor of nuclear factor kappa B kinase epsilon subunit	6.8	6.8	6.8	pKd	3
Homo sapiens	Interferon-induced, double-stranded RNA-activated protein kinase	5.2	5.2	5.2	pKd	3
Homo sapiens	Interleukin-1 receptor-associated kinase 3	6.8	6.8	6.8	pKd	3
Homo sapiens	Leucine-rich repeat serine/threonine-protein kinase 2	6.2	6.2	6.8	pKd	2
Homo sapiens	Leukocyte tyrosine kinase receptor	5.5	5.5	5.5	pKd	3
Homo sapiens	Macrophage colony stimulating factor receptor	6.9	6.9	6.9	pIC50	1
Homo sapiens	Macrophage colony stimulating factor receptor	6.5	6.5	6.5	pKd	3
Homo sapiens	MAP kinase signal-integrating kinase 2	6	6	6	pKd	3
Homo sapiens	MAP/microtubule affinity-regulating kinase 2	7	7	7	pKd	3
Homo sapiens	MAP/microtubule affinity-regulating kinase 4	6.4	6.4	6.4	pKd	3
Homo sapiens	Maternal embryonic leucine zipper kinase	6.6	6.6	6.6	pKd	3
Homo sapiens	Microtubule-associated serine/threonine-protein kinase 1	6.7	6.7	6.7	pKd	1
Homo sapiens	Misshapen-like kinase 1	6.8	6.8	6.8	pKd	2
Homo sapiens	Mitogen-activated protein kinase kinase kinase 10	6.1	6.1	6.1	pKd	2
Homo sapiens	Mitogen-activated protein kinase kinase kinase 11	7.8	6.1	7.8	pKd	4
Homo sapiens	Mitogen-activated protein kinase kinase kinase 13	5.6	5.6	5.6	pKd	2
Homo sapiens	Mitogen-activated protein kinase kinase kinase 2	6.9	6.9	6.9	pKd	2
Homo sapiens	Mitogen-activated protein kinase kinase kinase 3	6.7	6.7	6.7	pKd	2
Homo sapiens	Mitogen-activated protein kinase kinase kinase 7	6.9	6.9	6.9	pKd	2
Homo sapiens	Mitogen-activated protein kinase kinase kinase 9	7.8	7.8	7.8	pKd	3
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 1	5.7	5.7	5.7	pKd	3
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 2	5.6	5.6	5.6	pKd	2
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 3	6.9	6.9	6.9	pKd	3
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 4	6.2	6.2	6.2	pKd	3
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 5	6	6	6	pKd	3
Homo sapiens	Myosin light chain kinase	5.6	5.6	5.6	pKd	1
Homo sapiens	Myosin light chain kinase family member 4	6.2	6.2	6.2	pKd	3
Homo sapiens	Myosin light chain kinase, smooth muscle	5.7	5.7	6.7	pKd	3
Homo sapiens	Myotonin-protein kinase	5.4	5.4	5.4	pKd	3
Homo sapiens	Nerve growth factor receptor Trk-A	6.4	6.4	6.4	pKd	3
Homo sapiens	Neurotrophic tyrosine kinase receptor type 2	6.5	6.5	6.5	pKd	3
Homo sapiens	Non-receptor tyrosine-protein kinase TNK1	7.1	7.1	7.1	pKd	3
Homo sapiens	NT-3 growth factor receptor	5.8	5.8	5.8	pKd	3
Homo sapiens	NUAK family SNF1-like kinase 1	7.4	7.4	7.4	pKd	3
Homo sapiens	NUAK family SNF1-like kinase 2	7.2	7.2	7.2	pKd	3
Homo sapiens	Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha	6.5	6.5	6.5	pKd	3
Homo sapiens	Phosphatidylinositol-5-phosphate 4-kinase type-2 beta	6.6	6.6	6.6	pKd	3
Homo sapiens	Phosphorylase kinase gamma subunit 1	6.1	6.1	6.7	pKd	3
Homo sapiens	Phosphorylase kinase gamma subunit 2	5.9	5.9	6.1	pKd	3
Homo sapiens	Platelet-derived growth factor receptor alpha	6.4	6.4	6.8	pKd	3
Homo sapiens	Platelet-derived growth factor receptor beta	7	6.4	7	pKd	3
Homo sapiens	Protein kinase C alpha	6.1	6.1	6.1	pKd	1
Homo sapiens	Protein kinase C delta	6.5	6.5	6.5	pKd	3
Homo sapiens	Protein kinase C epsilon	6.3	6.3	6.3	pKd	3
Homo sapiens	Protein kinase C eta	6.5	6.5	6.5	pKd	3
Homo sapiens	Protein kinase C nu	5.5	5.5	5.5	pKd	3
Homo sapiens	Protein kinase C theta	6	6	6	pKd	3
Homo sapiens	Protein kinase N1	8	8	8	pKd	3
Homo sapiens	Protein kinase N2	7.8	7.8	7.8	pKd	3
Homo sapiens	Protein tyrosine kinase 2 beta	6.2	6.2	6.2	pKd	3
Homo sapiens	Proto-oncogene tyrosine-protein kinase MER	5.5	5.5	5.5	pKd	3
Homo sapiens	Proto-oncogene tyrosine-protein kinase ROS	6.4	6.4	6.4	pKd	3
Homo sapiens	Receptor protein-tyrosine kinase erbB-4	5.5	5.5	5.5	pKd	2
Homo sapiens	Rho-associated protein kinase 1	5.1	5.1	5.1	pKd	2
Homo sapiens	Rho-associated protein kinase 2	5.6	5.6	5.6	pKd	2
Homo sapiens	Rhodopsin kinase	7.1	7.1	7.1	pKd	2
Homo sapiens	Ribosomal protein S6 kinase 1	5.9	5.9	5.9	pKd	1
Homo sapiens	Ribosomal protein S6 kinase alpha 1	6.2	6.1	6.6	pKd	8
Homo sapiens	Ribosomal protein S6 kinase alpha 2	6.1	6.1	6.6	pKd	6
Homo sapiens	Ribosomal protein S6 kinase alpha 3	6.1	6.1	6.1	pKd	4
Homo sapiens	Ribosomal protein S6 kinase alpha 4	6.6	6.2	6.6	pKd	6
Homo sapiens	Ribosomal protein S6 kinase alpha 5	6.6	6.1	6.6	pKd	6
Homo sapiens	Ribosomal protein S6 kinase alpha 6	5.8	5.8	5.8	pKd	6
Homo sapiens	Serine/threonine-protein kinase 10	6.2	6.2	6.2	pKd	3
Homo sapiens	Serine/threonine-protein kinase 11	6.5	6.5	6.5	pKd	3
Homo sapiens	Serine/threonine-protein kinase 16	6.6	6.6	6.6	pKd	3
Homo sapiens	Serine/threonine-protein kinase 17A	5.7	5.7	5.7	pKd	3
Homo sapiens	Serine/threonine-protein kinase 17B	5.3	5.3	5.3	pKd	3
Homo sapiens	Serine/threonine-protein kinase 2	6.7	6.7	6.7	pKd	3
Homo sapiens	Serine/threonine-protein kinase 25	5.8	5.8	5.8	pKd	3
Homo sapiens	Serine/threonine-protein kinase 32A	5.6	5.6	5.6	pKd	1
Homo sapiens	Serine/threonine-protein kinase 33	6.7	6.7	6.7	pKd	1
Homo sapiens	Serine/threonine-protein kinase AKT	6	6	6	pKd	3
Homo sapiens	Serine/threonine-protein kinase AKT2	6.1	6.1	6.1	pKd	3
Homo sapiens	Serine/threonine-protein kinase AKT3	5.4	5.4	5.4	pKd	1
Homo sapiens	Serine/threonine-protein kinase Aurora-A	7.1	7.1	7.1	pIC50	1
Homo sapiens	Serine/threonine-protein kinase Aurora-A	6.9	6.9	7.7	pKd	4
Homo sapiens	Serine/threonine-protein kinase Aurora-B	7.2	7.2	7.2	pKd	3
Homo sapiens	Serine/threonine-protein kinase Aurora-C	6.8	6.8	6.8	pKd	3
Homo sapiens	Serine/threonine-protein kinase Chk1	5.9	5.9	5.9	pKd	3
Homo sapiens	Serine/threonine-protein kinase c-TAK1	7.7	7.7	7.7	pKd	3
Homo sapiens	Serine/threonine-protein kinase D2	5.2	5.2	5.2	pKd	3
Homo sapiens	Serine/threonine-protein kinase GAK	6.4	6.4	6.4	pKd	3
Homo sapiens	Serine/threonine-protein kinase ICK	5.2	5.2	5.2	pKd	1
Homo sapiens	Serine/threonine-protein kinase LATS1	6	6	6	pKd	3
Homo sapiens	Serine/threonine-protein kinase LATS2	5.7	5.7	5.7	pKd	3
Homo sapiens	Serine/threonine-protein kinase MARK1	6.8	6.8	6.8	pKd	3
Homo sapiens	Serine/threonine-protein kinase MST1	7.4	7.4	7.8	pKd	3
Homo sapiens	Serine/threonine-protein kinase MST2	6.7	6.7	7.4	pKd	3
Homo sapiens	Serine/threonine-protein kinase PAK 1	5.7	5.7	5.7	pKd	3
Homo sapiens	Serine/threonine-protein kinase PAK 2	5.5	5.5	5.7	pKd	3
Homo sapiens	Serine/threonine-protein kinase PAK 3	6.8	5.5	6.8	pKd	3
Homo sapiens	Serine/threonine-protein kinase PIM1	6.3	6.3	6.3	pKd	3
Homo sapiens	Serine/threonine-protein kinase PIM2	5.5	5.5	5.5	pKd	1
Homo sapiens	Serine/threonine-protein kinase PIM3	6.3	6.3	6.3	pKd	3
Homo sapiens	Serine/threonine-protein kinase PLK4	7.2	7.2	7.2	pKd	3
Homo sapiens	Serine/threonine-protein kinase PRKX	6	6	6	pKd	3
Homo sapiens	Serine/threonine-protein kinase PRP4 homolog	7.3	7.3	7.3	pKd	1
Homo sapiens	Serine/threonine-protein kinase RIO1	5.9	5.9	5.9	pKd	3
Homo sapiens	Serine/threonine-protein kinase RIO2	7	7	7	pKd	2
Homo sapiens	Serine/threonine-protein kinase RIO3	6.4	6.4	6.4	pKd	3
Homo sapiens	Serine/threonine-protein kinase Sgk3	5.9	5.9	5.9	pKd	1
Homo sapiens	Serine/threonine-protein kinase SIK1	6.8	6.8	6.8	pKd	3
Homo sapiens	Serine/threonine-protein kinase SIK2	6.3	6.3	6.3	pKd	3
Homo sapiens	Serine/threonine-protein kinase SIK3	7	7	7	pKd	2
Homo sapiens	Serine/threonine-protein kinase SRPK1	7.4	7.4	7.4	pKd	3
Homo sapiens	Serine/threonine-protein kinase SRPK2	6.5	6.5	6.5	pKd	3
Homo sapiens	Serine/threonine-protein kinase SRPK3	7.1	7.1	7.1	pKd	2
Homo sapiens	Serine/threonine-protein kinase TBK1	8	8	8	pKd	2
Homo sapiens	Serine/threonine-protein kinase ULK3	5.9	5.9	5.9	pKd	2
Homo sapiens	Serine/threonine-protein kinase WEE1	5.3	5.3	5.3	pKd	1
Homo sapiens	SPS1/STE20-related protein kinase YSK4	7.8	7.8	7.8	pKd	2
Homo sapiens	Stem cell growth factor receptor	6.8	5.8	8.1	pKd	21
Homo sapiens	Testis-specific serine/threonine-protein kinase 1	5.3	5.3	5.3	pKd	3
Homo sapiens	TRAF2- and NCK-interacting kinase	5.8	5.8	5.8	pKd	3
Homo sapiens	Tyrosine kinase non-receptor protein 2	6.9	6.9	6.9	pKd	2
Homo sapiens	Tyrosine-protein kinase ABL	5.5	5.3	6.1	pKd	6
Homo sapiens	Tyrosine-protein kinase BLK	6.3	6.3	6.3	pKd	3
Homo sapiens	Tyrosine-protein kinase CSK	5.1	5.1	5.1	pKd	3
Homo sapiens	Tyrosine-protein kinase FER	5.9	5.9	5.9	pKd	3
Homo sapiens	Tyrosine-protein kinase FGR	6.1	6.1	6.1	pKd	3
Homo sapiens	Tyrosine-protein kinase FRK	5.6	5.6	5.6	pKd	3
Homo sapiens	Tyrosine-protein kinase FYN	5.7	5.7	5.7	pKd	3
Homo sapiens	Tyrosine-protein kinase HCK	6.1	6.1	6.1	pKd	3
Homo sapiens	Tyrosine-protein kinase JAK1	6.2	6.2	6.2	pKd	4
Homo sapiens	Tyrosine-protein kinase JAK2	7	7	7	pKd	6
Homo sapiens	Tyrosine-protein kinase JAK3	7.9	7.9	7.9	pKd	6
Homo sapiens	Tyrosine-protein kinase LCK	6.6	6.6	6.6	pKd	3
Homo sapiens	Tyrosine-protein kinase Lyn	5.4	5.4	5.4	pKd	3
Homo sapiens	Tyrosine-protein kinase receptor FLT3	7.8	7.4	7.9	pIC50	3
Homo sapiens	Tyrosine-protein kinase receptor FLT3	8	7.8	8.7	pKd	19
Homo sapiens	Tyrosine-protein kinase receptor RET	6.9	6.5	7.7	pKd	10
Homo sapiens	Tyrosine-protein kinase receptor Tie-1	5.9	5.9	5.9	pKd	3
Homo sapiens	Tyrosine-protein kinase receptor UFO	6.2	6.2	6.2	pKd	3
Homo sapiens	Tyrosine-protein kinase SRC	5.9	5.9	5.9	pKd	3
Homo sapiens	Tyrosine-protein kinase SYK	7.1	7.1	7.1	pKd	3
Homo sapiens	Tyrosine-protein kinase TIE-2	5.7	5.7	5.7	pKd	3
Homo sapiens	Tyrosine-protein kinase TYK2	6.6	6.6	6.6	pKd	6
Homo sapiens	Tyrosine-protein kinase YES	6	6	6	pKd	3
Homo sapiens	Tyrosine-protein kinase ZAP-70	5.6	5.6	5.6	pKd	3
Homo sapiens	Vascular endothelial growth factor receptor 1	6.4	6.4	6.4	pKd	3
Homo sapiens	Vascular endothelial growth factor receptor 2	6.6	6.6	6.6	pIC50	1
Homo sapiens	Vascular endothelial growth factor receptor 2	5.5	5.5	5.5	pKd	3
Homo sapiens	Vascular endothelial growth factor receptor 3	6.2	6.2	6.2	pKd	3

Kinase-ligand interaction pattern

• Hydrophobic • Aromatic face-to-face • Aromatic face-to-edge • H-bond donor • H-bond acceptor • Ionic positive • Ionic negative

g.l

III

αC

1 S
163

2 L
164

3 I
165

4 G
166

5 K
167

6 G
168

7 S
169

8 F
170

9 G
171

10 Q
172

11 V
173

12 V
174

13 K
175

14 V
185

15 A
186

16 I
187

17 K
188

18 I
189

19 I
190

20 Q
199

•

αC

b.l

21 A
200

22 Q
201

23 I
202

24 E
203

25 V
204

26 R
205

27 L
206

28 L
207

29 E
208

30 L
209

31 M
210

32 N
211

33 Y
219

34 Y
220

35 I
221

36 V
222

37 H
223

38 L
224

39 K
225

40 R
226

hinge

linker

αD

αE

41 H
227

42 C
235

43 L
236

44 V
237

45 F
238

46 E
239

47 M
240

48 L
241

49 S
242

50 _
_

51 Y
243

52 N
244

53 L
245

54 Y
246

55 D
247

56 L
248

57 L
249

58 R
250

59 N
251

60 A
277

•

••

αE

c.l

VII

VIII

61 T
278

62 P
279

63 E
280

64 L
281

65 S
282

66 I
283

67 I
284

68 H
285

69 C
286

70 D
287

71 L
288

72 K
289

73 P
290

74 E
291

75 N
292

76 I
293

77 L
294

78 L
295

79 I
305

80 V
306

•

DFG

a.l

81 D
307

82 F
308

83 G
309

84 S
310

85 S
311

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Interaction pattern search

Search KLIFS for kinase-ligand complexes with similar interaction patterns: