KLIFS - the kinase structures database

Version: 3.2
Updated: 29-Oct-2025

Kinase structure
Kinase:	MAPK14 (p38a)
Family:	MAPK
Group:	CMGC
Species:	HUMAN
IUPHAR/BPS ID:	1499
PDB:	3ZS5
sc-PDB:
KIDFamMap:	Search
Release date:	2012-07-04
PubMed:	22868770
Chain:	A
(Alternate) Model:	B
Orthosteric ligand:	SB2

More entries for 3ZS5
3ZS5	Alternative model:	A	Chain:	A

Structural information
DFG conformation:	out
αC-helix conformation:	in
G-rich loop angle (distance):	69.3° (21.2Å)
G-rich loop rotation:	44°
Quality Score:	8.2
Resolution:	1.6 Å
Missing Residues:	1
Missing Atoms:	2

Ligand binding mode
Pockets	Subpockets	Waters
front gate back	BP-I-A BP-I-B	Cluster I3	Ligand No	Protein Yes

Pocket alignment
Uniprot sequence:	SPVGSGAYGSVCAVAVKKLRTYRELRLLKHMKENVIGLLDVYLVTHLMG-ADLNNIVKCYIHSADIIHRDLKPSNLAVILDFGLA
Sequence structure:	SPVGSGAYGSVCAVAVKKLRTYRELRLLKHMKENVIGLLDVYLVTHLMG_ADLNNIVKCYIHSADIIHRDLKPSNLAVILDFGL_

Ligand affinity
ChEMBL ID:	CHEMBL10
Bioaffinities:	245 records for 62 kinases

Species	Kinase (ChEMBL naming)	Median	Min	Max	Type	Records
Homo sapiens	Activin receptor type-1B	5.5	5.5	5.5	pKd	2
Homo sapiens	Bone morphogenetic protein receptor type-1B	5.5	5.5	5.5	pKd	1
Plasmodium falciparum (isolate 3D7)	Calcium-dependent protein kinase 1	6.1	6.1	6.1	pKd	1
Homo sapiens	Casein kinase I alpha	7.1	7.1	7.1	pKd	1
Homo sapiens	Casein kinase I alpha	6.5	6.5	6.5	pKi	1
Homo sapiens	Casein kinase I delta	6.9	6.9	6.9	pIC50	2
Homo sapiens	Casein kinase I delta	7.4	7.4	7.4	pKd	2
Homo sapiens	Casein kinase I delta	7.4	7.4	7.4	pKi	1
Homo sapiens	Casein kinase I epsilon	7	6.7	7	pKd	3
Homo sapiens	Casein kinase I isoform alpha-like	5.8	5.8	5.8	pKd	2
Homo sapiens	Citron Rho-interacting kinase	6.4	6.4	6.4	pKd	2
Homo sapiens	c-Jun N-terminal kinase 1	6	5.9	6	pKd	3
Homo sapiens	c-Jun N-terminal kinase 1	5.9	5.9	5.9	pKi	1
Homo sapiens	c-Jun N-terminal kinase 2	5.7	5	6.6	pIC50	6
Homo sapiens	c-Jun N-terminal kinase 2	6.9	6.9	7	pKd	3
Homo sapiens	c-Jun N-terminal kinase 2	6.7	6.7	6.7	pKi	1
Homo sapiens	c-Jun N-terminal kinase 3	6.1	6.1	6.5	pIC50	2
Homo sapiens	c-Jun N-terminal kinase 3	7.5	7.4	7.5	pKd	3
Homo sapiens	c-Jun N-terminal kinase 3	7.1	7.1	7.6	pKi	2
Homo sapiens	Discoidin domain-containing receptor 2	5.3	5.3	5.3	pKd	2
Homo sapiens	Dual specificity mitogen-activated protein kinase kinase 2	5	5	5	pKd	2
Homo sapiens	Dual specificity mitogen-activated protein kinase kinase 5	5.9	5.9	5.9	pKd	1
Homo sapiens	Dual specificity protein kinase TTK	5.6	5.6	5.6	pKd	2
Homo sapiens	Ephrin type-A receptor 6	5.9	5.9	5.9	pKd	2
Homo sapiens	Ephrin type-B receptor 2	5.1	5.1	5.1	pIC50	1
Homo sapiens	Ephrin type-B receptor 2	5.2	5.2	5.2	pKd	1
Homo sapiens	Epidermal growth factor receptor erbB1	4	4	4	pIC50	1
Homo sapiens	Epidermal growth factor receptor erbB1	5.8	5.5	6.5	pKd	23
Homo sapiens	Epithelial discoidin domain-containing receptor 1	6	6	6	pKd	2
Homo sapiens	Glycogen synthase kinase-3 beta	5.8	5.8	5.8	pKd	2
Homo sapiens	Glycogen synthase kinase-3 beta	5.8	5.8	5.8	pKi	1
Homo sapiens	Hepatocyte growth factor receptor	5.1	5.1	5.1	pKd	1
Homo sapiens	MAP kinase p38 alpha	7.3	5.2	8	pIC50	47
Homo sapiens	MAP kinase p38 alpha	7.9	7.7	8.4	pKd	5
Homo sapiens	MAP kinase p38 alpha	7.7	7.2	7.8	pKi	3
Mus musculus	MAP kinase p38 alpha	7.7	7.7	7.7	pKd	1
Homo sapiens	MAP kinase p38 beta	6.4	6.3	7.9	pIC50	4
Homo sapiens	MAP kinase p38 beta	7.2	6.6	7.2	pKd	3
Homo sapiens	MAP kinase p38 gamma	5.8	5.8	5.8	pKd	3
Homo sapiens	Misshapen-like kinase 1	5.6	5.6	5.6	pKi	1
Toxoplasma gondii	Mitogen-activated protein kinase 1	7	7	7	pIC50	2
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 4	5.4	5.4	5.4	pKd	2
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 4	6.4	6.4	6.4	pKi	1
Homo sapiens	Mixed lineage kinase 7	5.4	5.4	5.4	pKd	2
Homo sapiens	Receptor protein-tyrosine kinase erbB-2	4.7	4.7	4.7	pIC50	1
Homo sapiens	Receptor protein-tyrosine kinase erbB-4	5.3	5.3	5.3	pKd	1
Homo sapiens	Ribosomal protein S6 kinase alpha 1	6.5	6.5	6.5	pKd	4
Homo sapiens	Ribosomal protein S6 kinase alpha 6	6.6	6.6	6.6	pKd	4
Homo sapiens	Serine/threonine-protein kinase 2	5.4	5.3	5.4	pKd	3
Homo sapiens	Serine/threonine-protein kinase 2	5.7	5.7	5.7	pKi	1
Homo sapiens	Serine/threonine-protein kinase 32A	5.9	5.9	5.9	pKd	1
Homo sapiens	Serine/threonine-protein kinase 32B	5.7	5.7	5.7	pKd	2
Homo sapiens	Serine/threonine-protein kinase 36	5.9	5.9	6.1	pKd	3
Homo sapiens	Serine/threonine-protein kinase B-raf	6.2	6.2	6.3	pKd	4
Homo sapiens	Serine/threonine-protein kinase D2	5.2	5.2	5.2	pKi	1
Homo sapiens	Serine/threonine-protein kinase GAK	6.9	6.9	7	pIC50	2
Homo sapiens	Serine/threonine-protein kinase GAK	7.7	7.4	7.7	pKd	3
Homo sapiens	Serine/threonine-protein kinase MRCK-A	5.2	5.2	5.2	pKd	2
Homo sapiens	Serine/threonine-protein kinase MRCK beta	5.6	5.6	5.6	pKd	2
Homo sapiens	Serine/threonine-protein kinase MRCK gamma	6.4	6.4	6.4	pKd	2
Homo sapiens	Serine/threonine protein kinase NLK	7.6	7.6	7.6	pKd	2
Homo sapiens	Serine/threonine-protein kinase RAF	6.4	5.7	6.5	pIC50	4
Homo sapiens	Serine/threonine-protein kinase RAF	6	6	6	pKd	2
Homo sapiens	Serine/threonine-protein kinase RIPK2	6.6	5.2	8	pIC50	7
Homo sapiens	Serine/threonine-protein kinase RIPK2	7.6	5.7	7.6	pKd	3
Homo sapiens	Serine/threonine-protein kinase SIK2	6	6	6	pKi	1
Homo sapiens	Serine/threonine-protein kinase SIK3	5.4	5.4	5.4	pKd	1
Homo sapiens	Serine/threonine-protein kinase TNNI3K	5.5	5.5	5.5	pKd	2
Homo sapiens	TGF-beta receptor type I	4.5	4.5	5.2	pIC50	2
Homo sapiens	TGF-beta receptor type I	5.2	5.2	5.2	pKd	2
Homo sapiens	TGF-beta receptor type II	5.8	5.8	5.8	pKd	2
Homo sapiens	TRAF2- and NCK-interacting kinase	6.1	5.8	6.1	pKd	3
Homo sapiens	Tyrosine-protein kinase ABL	5.4	5.4	5.6	pKd	2
Homo sapiens	Tyrosine-protein kinase BRK	5.4	5.1	5.4	pKd	3
Homo sapiens	Tyrosine-protein kinase FRK	5.8	5.4	5.8	pKd	3
Homo sapiens	Tyrosine-protein kinase FRK	6.6	6.6	6.6	pKi	1
Homo sapiens	Tyrosine-protein kinase LCK	5.5	5.5	5.5	pIC50	1
Homo sapiens	Tyrosine-protein kinase LCK	5.6	5.6	5.6	pKd	2
Homo sapiens	Tyrosine-protein kinase LCK	5.3	5.3	5.3	pKi	1
Homo sapiens	Tyrosine-protein kinase SRC	5.3	5.3	5.3	pIC50	1
Homo sapiens	Tyrosine-protein kinase SRC	5.3	5.3	5.3	pKd	2
Homo sapiens	Tyrosine-protein kinase TIE-2	4.7	4.7	4.7	pIC50	1
Homo sapiens	Tyrosine-protein kinase TXK	5.4	5.4	5.4	pKd	2
Homo sapiens	Tyrosine-protein kinase TYK2	5.7	5.7	5.7	pKd	2
Homo sapiens	Uncharacterized aarF domain-containing protein kinase 4	5.5	5.5	5.5	pKd	2
Homo sapiens	Vascular endothelial growth factor receptor 3	5.2	5.2	5.2	pKi	1

Kinase-ligand interaction pattern

• Hydrophobic • Aromatic face-to-face • Aromatic face-to-edge • H-bond donor • H-bond acceptor • Ionic positive • Ionic negative

g.l

III

αC

1 S
28

2 P
29

3 V
30

4 G
31

5 S
32

6 G
33

7 A
34

8 Y
35

9 G
36

10 S
37

11 V
38

12 C
39

13 A
40

14 V
50

15 A
51

16 V
52

17 K
53

18 K
54

19 L
55

20 R
67

••

•

••

αC

b.l

21 T
68

22 Y
69

23 R
70

24 E
71

25 L
72

26 R
73

27 L
74

28 L
75

29 K
76

30 H
77

31 M
78

32 K
79

33 E
81

34 N
82

35 V
83

36 I
84

37 G
85

38 L
86

39 L
87

40 D
88

•

hinge

linker

αD

αE

41 V
89

42 Y
103

43 L
104

44 V
105

45 T
106

46 H
107

47 L
108

48 M
109

49 G
110

50 _
_

51 A
111

52 D
112

53 L
113

54 N
114

55 N
115

56 I
116

57 V
117

58 K
118

59 C
119

60 Y
140

•

••

αE

c.l

VII

VIII

61 I
141

62 H
142

63 S
143

64 A
144

65 D
145

66 I
146

67 I
147

68 H
148

69 R
149

70 D
150

71 L
151

72 K
152

73 P
153

74 S
154

75 N
155

76 L
156

77 A
157

78 V
158

79 I
166

80 L
167

DFG

a.l

81 D
168

82 F
169

83 G
170

84 L
171

85 _
_

•••

•

Interaction pattern search

Search KLIFS for kinase-ligand complexes with similar interaction patterns: