KLIFS - the kinase structures database

Version: 3.2
Updated: 01-Nov-2025

Kinase structure
Kinase:	MAPK14 (p38a)
Family:	MAPK
Group:	CMGC
Species:	HUMAN
IUPHAR/BPS ID:	1499
PDB:	3L8S
sc-PDB:
KIDFamMap:	Search
Release date:	2010-03-09
PubMed:	20201574
Chain:	A
Orthosteric ligand:	BFF

Structural information
DFG conformation:	out
αC-helix conformation:	in
G-rich loop angle (distance):	-
G-rich loop rotation:	-
Quality Score:	7.2
Resolution:	2.35 Å
Missing Residues:	6
Missing Atoms:	4

Ligand binding mode
Pockets	Subpockets
front gate back	BP-I-A BP-I-B

Pocket alignment
Uniprot sequence:	SPVGSGAYGSVCAVAVKKLRTYRELRLLKHMKENVIGLLDVYLVTHLMG-ADLNNIVKCYIHSADIIHRDLKPSNLAVILDFGLA
Sequence structure:	SPVGSG___SVCAVAVKKLRTYRELRLLKHMKENVIGLLDVYLVTHLMG_ADLNNIVKSYIHSADIIHRDLKPSNLAVILDF___

Ligand affinity
ChEMBL ID:	CHEMBL253969
Bioaffinities:	68 records for 48 kinases

Species	Kinase (ChEMBL naming)	Median	Min	Max	Type	Records
Homo sapiens	Activin receptor type-1	6.4	6.4	6.4	pKi	1
Homo sapiens	ALK tyrosine kinase receptor	6.1	6.1	6.1	pKi	1
Homo sapiens	BR serine/threonine-protein kinase 1	5.2	5.2	5.2	pKi	1
Homo sapiens	c-Jun N-terminal kinase 2	6	6	6	pKi	1
Homo sapiens	Discoidin domain-containing receptor 2	6.4	6.4	7.6	pIC50	2
Homo sapiens	Discoidin domain-containing receptor 2	6.4	6.4	6.4	pKd	1
Homo sapiens	Ephrin type-A receptor 2	6.3	6.3	6.3	pKi	1
Homo sapiens	Epidermal growth factor receptor erbB1	5.2	5.2	5.2	pIC50	1
Homo sapiens	Fibroblast growth factor receptor 1	8.1	8.1	8.1	pIC50	1
Homo sapiens	Fibroblast growth factor receptor 1	7.8	7.8	7.8	pKi	1
Homo sapiens	Fibroblast growth factor receptor 2	8.1	8.1	8.5	pIC50	2
Homo sapiens	Fibroblast growth factor receptor 3	7.5	7.5	7.5	pIC50	1
Homo sapiens	Fibroblast growth factor receptor 3	8.1	8.1	8.1	pKi	1
Homo sapiens	Fibroblast growth factor receptor 4	6.3	6.3	6.3	pIC50	1
Homo sapiens	Hepatocyte growth factor receptor	5.7	5.7	5.7	pKi	1
Homo sapiens	Insulin receptor	5	5	5	pIC50	1
Homo sapiens	Leukocyte tyrosine kinase receptor	6.7	6.7	6.7	pKi	1
Homo sapiens	MAP kinase p38 alpha	6.5	6.5	6.5	pKi	1
Homo sapiens	MAP/microtubule affinity-regulating kinase 2	5.4	5.4	5.4	pKi	1
Homo sapiens	Misshapen-like kinase 1	6.6	6.6	6.6	pKi	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 2	5.8	5.8	5.8	pKi	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 4	6.8	6.8	6.8	pKi	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 5	6.6	6.6	6.6	pKi	1
Homo sapiens	Phosphorylase kinase gamma subunit 2	5.6	5.6	5.6	pKi	1
Homo sapiens	Platelet-derived growth factor receptor alpha	5.5	5.5	5.5	pIC50	1
Homo sapiens	Platelet-derived growth factor receptor alpha	6.4	6.4	6.4	pKi	1
Homo sapiens	Platelet-derived growth factor receptor beta	5.6	5.6	5.7	pIC50	2
Homo sapiens	Platelet-derived growth factor receptor beta	6.4	6.4	6.4	pKi	1
Homo sapiens	Proto-oncogene tyrosine-protein kinase ROS	6	6	6	pKi	1
Homo sapiens	Serine/threonine-protein kinase 2	8	8	8	pKi	1
Homo sapiens	Serine/threonine-protein kinase Aurora-A	6.2	6.2	6.2	pKi	1
Homo sapiens	Serine/threonine-protein kinase Aurora-B	6.7	6.7	6.7	pIC50	1
Homo sapiens	Serine/threonine-protein kinase Aurora-B	6.6	6.6	6.6	pKi	1
Homo sapiens	Serine/threonine-protein kinase c-TAK1	5.3	5.3	5.3	pKi	1
Homo sapiens	Serine/threonine-protein kinase PLK4	6.6	6.6	6.6	pKi	1
Homo sapiens	Serine/threonine-protein kinase TAO1	6.5	6.5	6.5	pKi	1
Homo sapiens	Tyrosine-protein kinase ABL	7.1	7.1	7.1	pKi	1
Homo sapiens	Tyrosine-protein kinase BLK	6.5	6.5	6.5	pKi	1
Homo sapiens	Tyrosine-protein kinase BRK	6.5	6.5	6.5	pKi	1
Homo sapiens	Tyrosine-protein kinase FER	5.8	5.8	5.8	pKi	1
Homo sapiens	Tyrosine-protein kinase FRK	7.6	7.6	7.6	pKi	1
Homo sapiens	Tyrosine-protein kinase FYN	6.8	6.8	6.8	pIC50	1
Homo sapiens	Tyrosine-protein kinase FYN	6.3	6.3	6.3	pKi	1
Homo sapiens	Tyrosine-protein kinase ITK/TSK	6.4	6.4	6.4	pKi	1
Homo sapiens	Tyrosine-protein kinase LCK	7	7	7	pKi	1
Homo sapiens	Tyrosine-protein kinase Lyn	7	7	7	pKi	1
Homo sapiens	Tyrosine-protein kinase receptor FLT3	6.4	6.4	6.4	pIC50	1
Homo sapiens	Tyrosine-protein kinase receptor RET	8	8	8	pIC50	1
Homo sapiens	Tyrosine-protein kinase receptor RET	7.5	7.5	7.5	pKi	1
Homo sapiens	Tyrosine-protein kinase receptor UFO	6.2	6.2	6.2	pKi	1
Homo sapiens	Tyrosine-protein kinase SRC	6.9	6.9	6.9	pKi	1
Homo sapiens	Tyrosine-protein kinase Srms	5.9	5.9	5.9	pKi	1
Homo sapiens	Tyrosine-protein kinase TIE-2	7.8	6.2	8	pIC50	3
Homo sapiens	Vascular endothelial growth factor receptor 1	6.9	6.9	6.9	pIC50	1
Homo sapiens	Vascular endothelial growth factor receptor 1	7.6	7.6	7.6	pKi	1
Homo sapiens	Vascular endothelial growth factor receptor 2	8	8	8.9	pIC50	5
Homo sapiens	Vascular endothelial growth factor receptor 2	8.6	8.6	8.6	pKi	1
Homo sapiens	Vascular endothelial growth factor receptor 3	7.3	7.3	7.3	pIC50	1
Homo sapiens	Vascular endothelial growth factor receptor 3	7	7	7	pKi	1

Kinase-ligand interaction pattern

• Hydrophobic • Aromatic face-to-face • Aromatic face-to-edge • H-bond donor • H-bond acceptor • Ionic positive • Ionic negative

g.l

III

αC

1 S
28

2 P
29

3 V
30

4 G
31

5 S
32

6 G
36

7 _
_

8 _
_

9 _
_

10 S
37

11 V
38

12 C
39

13 A
40

14 V
50

15 A
51

16 V
52

17 K
53

18 K
54

19 L
55

20 R
67

•

αC

b.l

21 T
68

22 Y
69

23 R
70

24 E
71

25 L
72

26 R
73

27 L
74

28 L
75

29 K
76

30 H
77

31 M
78

32 K
79

33 E
81

34 N
82

35 V
83

36 I
84

37 G
85

38 L
86

39 L
87

40 D
88

•

hinge

linker

αD

αE

41 V
89

42 Y
103

43 L
104

44 V
105

45 T
106

46 H
107

47 L
108

48 M
109

49 G
110

50 _
_

51 A
111

52 D
112

53 L
113

54 N
114

55 N
115

56 I
116

57 V
117

58 K
118

59 S
119

60 Y
140

•

•••

•

αE

c.l

VII

VIII

61 I
141

62 H
142

63 S
143

64 A
144

65 D
145

66 I
146

67 I
147

68 H
148

69 R
149

70 D
150

71 L
151

72 K
152

73 P
153

74 S
154

75 N
155

76 L
156

77 A
157

78 V
158

79 I
166

80 L
167

•

DFG

a.l

81 D
168

82 F
169

83 _
_

84 _
_

85 _
_

•

Interaction pattern search

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