KLIFS - the kinase structures database

Version: 3.2
Updated: 29-Oct-2025

B-Raf proto-oncogene, serine/threonine kinase

Kinase structure
Kinase:	BRAF
Family:	RAF
Group:	TKL
Species:	HUMAN
IUPHAR/BPS ID:	1943
PDB:	5CT7
sc-PDB:
KIDFamMap:	Search
Release date:	2015-09-09
PubMed:	26396681
Chain:	A
Orthosteric ligand:	55J

More entries for 5CT7
5CT7	Chain:	B

Structural information
DFG conformation:	out
αC-helix conformation:	in
G-rich loop angle (distance):	56.3° (17.6Å)
G-rich loop rotation:	28.3°
Quality Score:	8.6
Resolution:	3.17 Å
Missing Residues:	0
Missing Atoms:	6

Ligand binding mode
Pockets	Subpockets
front gate back	BP-I-B BP-II-out BP-III

Pocket alignment
Uniprot sequence:	QRIGSGSFGTVYKVAVKMLAFKNEVGVLRKTRVNILLFMGYAIVTQWCEGSSLYHHLHIYLHAKSIIHRDLKSNNIFLIGDFGLA
Sequence structure:	QRIGSGSFGTVYKVAVKMLAFKNEVGVLRKTRVNILLFMGYAIVTQWCEGSSLYHHLHIYLHAKSIIHRDLKSNNIFLIGDFGLA

Ligand affinity
ChEMBL ID:	CHEMBL558752
Bioaffinities:	157 records for 65 kinases

Species	Kinase (ChEMBL naming)	Median	Min	Max	Type	Records
Homo sapiens	AMP-activated protein kinase, alpha-1 subunit	5.4	5.4	5.4	pKd	2
Homo sapiens	Cell division cycle 2-like protein kinase 6	5.3	5.3	5.3	pKd	2
Homo sapiens	Citron Rho-interacting kinase	7.1	7.1	7.1	pKd	2
Homo sapiens	Discoidin domain-containing receptor 2	6	6	6	pKd	2
Homo sapiens	Dual specificity mitogen-activated protein kinase kinase 5	5.7	5.7	5.7	pKd	1
Homo sapiens	Ephrin type-A receptor 1	5.1	5.1	5.1	pKd	1
Homo sapiens	Ephrin type-A receptor 2	5.5	5.5	5.5	pKd	2
Homo sapiens	Ephrin type-A receptor 4	5.1	5.1	5.1	pKd	1
Homo sapiens	Ephrin type-A receptor 6	5.8	5.8	5.8	pKd	2
Homo sapiens	Ephrin type-A receptor 8	6	6	6	pKd	2
Homo sapiens	Epithelial discoidin domain-containing receptor 1	7.9	7.9	7.9	pKd	2
Homo sapiens	Eukaryotic translation initiation factor 2-alpha kinase 4	7.3	7.3	7.3	pKd	2
Homo sapiens	Fibroblast growth factor receptor 1	5.3	5.3	5.3	pKd	2
Homo sapiens	Hepatocyte growth factor receptor	5.2	5.2	5.9	pKd	2
Homo sapiens	Homeodomain-interacting protein kinase 4	6.3	6.3	6.3	pKd	1
Homo sapiens	Interferon-induced, double-stranded RNA-activated protein kinase	6	6	6	pKd	2
Homo sapiens	Macrophage colony stimulating factor receptor	6.6	6.6	6.6	pKd	2
Homo sapiens	Mitogen-activated protein kinase kinase kinase 11	5	5	5	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase 7	6.3	6.3	6.3	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 2	5.3	5.3	5.3	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 3	5.1	5.1	5.1	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 4	5.9	5.9	5.9	pKd	2
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 5	6.1	6.1	6.1	pKd	2
Homo sapiens	Mixed lineage kinase 7	7.2	7.2	7.2	pKd	2
Homo sapiens	Non-receptor tyrosine-protein kinase TNK1	5.2	5.2	5.2	pKd	1
Homo sapiens	Platelet-derived growth factor receptor alpha	6	6	6	pKd	2
Homo sapiens	Platelet-derived growth factor receptor beta	8.2	8.2	8.2	pIC50	1
Homo sapiens	Platelet-derived growth factor receptor beta	6.6	6.6	6.6	pKd	2
Homo sapiens	Receptor-interacting serine/threonine-protein kinase 1	5.3	5.3	5.3	pKd	1
Homo sapiens	Serine/threonine-protein kinase 10	7.2	7.2	7.2	pKd	2
Homo sapiens	Serine/threonine-protein kinase 2	6.3	6.3	6.3	pKd	2
Homo sapiens	Serine/threonine-protein kinase 25	5.2	5.2	5.2	pKd	1
Homo sapiens	Serine/threonine-protein kinase 36	5.1	5.1	5.1	pKd	1
Homo sapiens	Serine/threonine-protein kinase B-raf	7.4	6.9	9.3	pIC50	6
Homo sapiens	Serine/threonine-protein kinase B-raf	5.9	5.9	6.5	pKd	4
Homo sapiens	Serine/threonine-protein kinase Nek5	5.5	5.5	5.5	pKd	2
Homo sapiens	Serine/threonine-protein kinase PAK 3	6.1	6.1	6.1	pKd	1
Homo sapiens	Serine/threonine-protein kinase RAF	5.9	5.9	7.7	pIC50	2
Homo sapiens	Serine/threonine-protein kinase RAF	6.4	6.4	6.4	pKd	2
Homo sapiens	Serine/threonine-protein kinase RIPK2	6	6	6	pKd	2
Homo sapiens	Serine/threonine-protein kinase TAO1	5.4	5.4	5.4	pKd	1
Homo sapiens	Serine/threonine-protein kinase TAO2	6.9	6.9	6.9	pKd	1
Homo sapiens	Serine/threonine-protein kinase TAO3	6.1	6.1	6.1	pKd	1
Homo sapiens	Serine/threonine-protein kinase TNNI3K	5.2	5.2	5.2	pKd	2
Homo sapiens	SPS1/STE20-related protein kinase YSK4	7.3	7.3	7.3	pKd	1
Homo sapiens	Stem cell growth factor receptor	7.7	7.7	7.7	pIC50	1
Homo sapiens	Stem cell growth factor receptor	6.6	5.1	7.1	pKd	15
Homo sapiens	TRAF2- and NCK-interacting kinase	5.8	5.8	5.8	pKd	2
Homo sapiens	Tyrosine-protein kinase ABL	5.8	5.1	6.7	pKd	18
Homo sapiens	Tyrosine-protein kinase ABL2	5.7	5.7	5.7	pKd	2
Homo sapiens	Tyrosine-protein kinase BLK	5.1	5.1	5.1	pKd	1
Homo sapiens	Tyrosine-protein kinase BRK	5.4	5.4	5.4	pKd	2
Homo sapiens	Tyrosine-protein kinase FER	5.1	5.1	5.1	pKd	1
Homo sapiens	Tyrosine-protein kinase FRK	6.2	6.2	6.2	pKd	2
Homo sapiens	Tyrosine-protein kinase FYN	5.7	5.7	5.7	pKd	2
Homo sapiens	Tyrosine-protein kinase HCK	5.9	5.9	5.9	pKd	2
Homo sapiens	Tyrosine-protein kinase LCK	6.2	6.2	6.2	pKd	2
Homo sapiens	Tyrosine-protein kinase Lyn	6.4	6.4	6.4	pKd	2
Homo sapiens	Tyrosine-protein kinase receptor FLT3	5.9	5.9	6.1	pKd	3
Homo sapiens	Tyrosine-protein kinase receptor RET	6.8	6.5	7.1	pKd	6
Homo sapiens	Tyrosine-protein kinase receptor Tie-1	6.8	6.8	6.8	pKd	2
Homo sapiens	Tyrosine-protein kinase SRC	6	6	6	pKd	2
Homo sapiens	Tyrosine-protein kinase SYK	5.4	5.4	5.4	pKd	1
Homo sapiens	Tyrosine-protein kinase TIE-2	5.9	5.9	5.9	pKd	2
Homo sapiens	Tyrosine-protein kinase TYK2	6.4	6.4	6.4	pKd	2
Homo sapiens	Tyrosine-protein kinase YES	6	6	6	pKd	2
Homo sapiens	Vascular endothelial growth factor receptor 1	6.1	6.1	6.1	pKd	2
Homo sapiens	Vascular endothelial growth factor receptor 2	5.9	5.9	5.9	pIC50	1
Homo sapiens	Vascular endothelial growth factor receptor 2	5.9	5.9	5.9	pKd	2
Homo sapiens	Vascular endothelial growth factor receptor 3	5.7	5.7	5.7	pKd	2

Kinase-ligand interaction pattern

• Hydrophobic • Aromatic face-to-face • Aromatic face-to-edge • H-bond donor • H-bond acceptor • Ionic positive • Ionic negative

g.l

III

αC

1 Q
461

2 R
462

3 I
463

4 G
464

5 S
465

6 G
466

7 S
467

8 F
468

9 G
469

10 T
470

11 V
471

12 Y
472

13 K
473

14 V
480

15 A
481

16 V
482

17 K
483

18 M
484

19 L
485

20 A
497

•

αC

b.l

21 F
498

22 K
499

23 N
500

24 E
501

25 V
502

26 G
503

27 V
504

28 L
505

29 R
506

30 K
507

31 T
508

32 R
509

33 V
511

34 N
512

35 I
513

36 L
514

37 L
515

38 F
516

39 M
517

40 G
518

••

•

hinge

linker

αD

αE

41 Y
519

42 A
526

43 I
527

44 V
528

45 T
529

46 Q
530

47 W
531

48 C
532

49 E
533

50 G
534

51 S
535

52 S
536

53 L
537

54 Y
538

55 H
539

56 H
540

57 L
541

58 H
542

59 I
543

60 Y
566

•

••

•

αE

c.l

VII

VIII

61 L
567

62 H
568

63 A
569

64 K
570

65 S
571

66 I
572

67 I
573

68 H
574

69 R
575

70 D
576

71 L
577

72 K
578

73 S
579

74 N
580

75 N
581

76 I
582

77 F
583

78 L
584

79 I
592

80 G
593

•

••

•

DFG

a.l

81 D
594

82 F
595

83 G
596

84 L
597

85 A
598

••

Interaction pattern search

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