KLIFS - the kinase structures database

Version: 3.2
Updated: 29-Oct-2025

Receptor interacting serine/threonine kinase 2

Kinase structure
Kinase:	RIPK2
Family:	RIPK
Group:	TKL
Species:	HUMAN
IUPHAR/BPS ID:	2190
PDB:	5AR4
sc-PDB:
KIDFamMap:	Search
Release date:	2015-10-21
PubMed:	26455654
Chain:	B
Orthosteric ligand:	SB2

More entries for 5AR4
5AR4	Chain:	A

Structural information
DFG conformation:	in
αC-helix conformation:	in
G-rich loop angle (distance):	49.5° (15.2Å)
G-rich loop rotation:	75.2°
Quality Score:	7.6
Resolution:	2.7 Å
Missing Residues:	1
Missing Atoms:	0

Ligand binding mode
Pockets	Subpockets	Waters
front gate back	BP-I-A BP-I-B	Cluster I1 I4 I10	Ligand No No Yes	Protein Yes No Yes

Pocket alignment
Uniprot sequence:	RYLSRGASGTVSSVAVKHLDVLREAEILHKARSYILPILGIGIVTEYMPNGSLNELLHRHNMTPPLLHHDLKTQNILLIADFGLS
Sequence structure:	RYLSRGASGTVSSVAVKHLDVLREAEILHKARSYILPILGIGIVTEYMPNGSLNELLHRHNMTPPLLHHDLKTQNILLIADFGL_

Ligand affinity
ChEMBL ID:	CHEMBL10
Bioaffinities:	245 records for 62 kinases

Species	Kinase (ChEMBL naming)	Median	Min	Max	Type	Records
Homo sapiens	Activin receptor type-1B	5.5	5.5	5.5	pKd	2
Homo sapiens	Bone morphogenetic protein receptor type-1B	5.5	5.5	5.5	pKd	1
Plasmodium falciparum (isolate 3D7)	Calcium-dependent protein kinase 1	6.1	6.1	6.1	pKd	1
Homo sapiens	Casein kinase I alpha	7.1	7.1	7.1	pKd	1
Homo sapiens	Casein kinase I alpha	6.5	6.5	6.5	pKi	1
Homo sapiens	Casein kinase I delta	6.9	6.9	6.9	pIC50	2
Homo sapiens	Casein kinase I delta	7.4	7.4	7.4	pKd	2
Homo sapiens	Casein kinase I delta	7.4	7.4	7.4	pKi	1
Homo sapiens	Casein kinase I epsilon	7	6.7	7	pKd	3
Homo sapiens	Casein kinase I isoform alpha-like	5.8	5.8	5.8	pKd	2
Homo sapiens	Citron Rho-interacting kinase	6.4	6.4	6.4	pKd	2
Homo sapiens	c-Jun N-terminal kinase 1	6	5.9	6	pKd	3
Homo sapiens	c-Jun N-terminal kinase 1	5.9	5.9	5.9	pKi	1
Homo sapiens	c-Jun N-terminal kinase 2	5.7	5	6.6	pIC50	6
Homo sapiens	c-Jun N-terminal kinase 2	6.9	6.9	7	pKd	3
Homo sapiens	c-Jun N-terminal kinase 2	6.7	6.7	6.7	pKi	1
Homo sapiens	c-Jun N-terminal kinase 3	6.1	6.1	6.5	pIC50	2
Homo sapiens	c-Jun N-terminal kinase 3	7.5	7.4	7.5	pKd	3
Homo sapiens	c-Jun N-terminal kinase 3	7.1	7.1	7.6	pKi	2
Homo sapiens	Discoidin domain-containing receptor 2	5.3	5.3	5.3	pKd	2
Homo sapiens	Dual specificity mitogen-activated protein kinase kinase 2	5	5	5	pKd	2
Homo sapiens	Dual specificity mitogen-activated protein kinase kinase 5	5.9	5.9	5.9	pKd	1
Homo sapiens	Dual specificity protein kinase TTK	5.6	5.6	5.6	pKd	2
Homo sapiens	Ephrin type-A receptor 6	5.9	5.9	5.9	pKd	2
Homo sapiens	Ephrin type-B receptor 2	5.1	5.1	5.1	pIC50	1
Homo sapiens	Ephrin type-B receptor 2	5.2	5.2	5.2	pKd	1
Homo sapiens	Epidermal growth factor receptor erbB1	4	4	4	pIC50	1
Homo sapiens	Epidermal growth factor receptor erbB1	5.8	5.5	6.5	pKd	23
Homo sapiens	Epithelial discoidin domain-containing receptor 1	6	6	6	pKd	2
Homo sapiens	Glycogen synthase kinase-3 beta	5.8	5.8	5.8	pKd	2
Homo sapiens	Glycogen synthase kinase-3 beta	5.8	5.8	5.8	pKi	1
Homo sapiens	Hepatocyte growth factor receptor	5.1	5.1	5.1	pKd	1
Homo sapiens	MAP kinase p38 alpha	7.3	5.2	8	pIC50	47
Homo sapiens	MAP kinase p38 alpha	7.9	7.7	8.4	pKd	5
Homo sapiens	MAP kinase p38 alpha	7.7	7.2	7.8	pKi	3
Mus musculus	MAP kinase p38 alpha	7.7	7.7	7.7	pKd	1
Homo sapiens	MAP kinase p38 beta	6.4	6.3	7.9	pIC50	4
Homo sapiens	MAP kinase p38 beta	7.2	6.6	7.2	pKd	3
Homo sapiens	MAP kinase p38 gamma	5.8	5.8	5.8	pKd	3
Homo sapiens	Misshapen-like kinase 1	5.6	5.6	5.6	pKi	1
Toxoplasma gondii	Mitogen-activated protein kinase 1	7	7	7	pIC50	2
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 4	5.4	5.4	5.4	pKd	2
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 4	6.4	6.4	6.4	pKi	1
Homo sapiens	Mixed lineage kinase 7	5.4	5.4	5.4	pKd	2
Homo sapiens	Receptor protein-tyrosine kinase erbB-2	4.7	4.7	4.7	pIC50	1
Homo sapiens	Receptor protein-tyrosine kinase erbB-4	5.3	5.3	5.3	pKd	1
Homo sapiens	Ribosomal protein S6 kinase alpha 1	6.5	6.5	6.5	pKd	4
Homo sapiens	Ribosomal protein S6 kinase alpha 6	6.6	6.6	6.6	pKd	4
Homo sapiens	Serine/threonine-protein kinase 2	5.4	5.3	5.4	pKd	3
Homo sapiens	Serine/threonine-protein kinase 2	5.7	5.7	5.7	pKi	1
Homo sapiens	Serine/threonine-protein kinase 32A	5.9	5.9	5.9	pKd	1
Homo sapiens	Serine/threonine-protein kinase 32B	5.7	5.7	5.7	pKd	2
Homo sapiens	Serine/threonine-protein kinase 36	5.9	5.9	6.1	pKd	3
Homo sapiens	Serine/threonine-protein kinase B-raf	6.2	6.2	6.3	pKd	4
Homo sapiens	Serine/threonine-protein kinase D2	5.2	5.2	5.2	pKi	1
Homo sapiens	Serine/threonine-protein kinase GAK	6.9	6.9	7	pIC50	2
Homo sapiens	Serine/threonine-protein kinase GAK	7.7	7.4	7.7	pKd	3
Homo sapiens	Serine/threonine-protein kinase MRCK-A	5.2	5.2	5.2	pKd	2
Homo sapiens	Serine/threonine-protein kinase MRCK beta	5.6	5.6	5.6	pKd	2
Homo sapiens	Serine/threonine-protein kinase MRCK gamma	6.4	6.4	6.4	pKd	2
Homo sapiens	Serine/threonine protein kinase NLK	7.6	7.6	7.6	pKd	2
Homo sapiens	Serine/threonine-protein kinase RAF	6.4	5.7	6.5	pIC50	4
Homo sapiens	Serine/threonine-protein kinase RAF	6	6	6	pKd	2
Homo sapiens	Serine/threonine-protein kinase RIPK2	6.6	5.2	8	pIC50	7
Homo sapiens	Serine/threonine-protein kinase RIPK2	7.6	5.7	7.6	pKd	3
Homo sapiens	Serine/threonine-protein kinase SIK2	6	6	6	pKi	1
Homo sapiens	Serine/threonine-protein kinase SIK3	5.4	5.4	5.4	pKd	1
Homo sapiens	Serine/threonine-protein kinase TNNI3K	5.5	5.5	5.5	pKd	2
Homo sapiens	TGF-beta receptor type I	4.5	4.5	5.2	pIC50	2
Homo sapiens	TGF-beta receptor type I	5.2	5.2	5.2	pKd	2
Homo sapiens	TGF-beta receptor type II	5.8	5.8	5.8	pKd	2
Homo sapiens	TRAF2- and NCK-interacting kinase	6.1	5.8	6.1	pKd	3
Homo sapiens	Tyrosine-protein kinase ABL	5.4	5.4	5.6	pKd	2
Homo sapiens	Tyrosine-protein kinase BRK	5.4	5.1	5.4	pKd	3
Homo sapiens	Tyrosine-protein kinase FRK	5.8	5.4	5.8	pKd	3
Homo sapiens	Tyrosine-protein kinase FRK	6.6	6.6	6.6	pKi	1
Homo sapiens	Tyrosine-protein kinase LCK	5.5	5.5	5.5	pIC50	1
Homo sapiens	Tyrosine-protein kinase LCK	5.6	5.6	5.6	pKd	2
Homo sapiens	Tyrosine-protein kinase LCK	5.3	5.3	5.3	pKi	1
Homo sapiens	Tyrosine-protein kinase SRC	5.3	5.3	5.3	pIC50	1
Homo sapiens	Tyrosine-protein kinase SRC	5.3	5.3	5.3	pKd	2
Homo sapiens	Tyrosine-protein kinase TIE-2	4.7	4.7	4.7	pIC50	1
Homo sapiens	Tyrosine-protein kinase TXK	5.4	5.4	5.4	pKd	2
Homo sapiens	Tyrosine-protein kinase TYK2	5.7	5.7	5.7	pKd	2
Homo sapiens	Uncharacterized aarF domain-containing protein kinase 4	5.5	5.5	5.5	pKd	2
Homo sapiens	Vascular endothelial growth factor receptor 3	5.2	5.2	5.2	pKi	1

Kinase-ligand interaction pattern

• Hydrophobic • Aromatic face-to-face • Aromatic face-to-edge • H-bond donor • H-bond acceptor • Ionic positive • Ionic negative

g.l

III

αC

1 R
22

2 Y
23

3 L
24

4 S
25

5 R
26

6 G
27

7 A
28

8 S
29

9 G
30

10 T
31

11 V
32

12 S
33

13 S
34

14 V
44

15 A
45

16 V
46

17 K
47

18 H
48

19 L
49

20 D
62

•

αC

b.l

21 V
63

22 L
64

23 R
65

24 E
66

25 A
67

26 E
68

27 I
69

28 L
70

29 H
71

30 K
72

31 A
73

32 R
74

33 S
76

34 Y
77

35 I
78

36 L
79

37 P
80

38 I
81

39 L
82

40 G
83

•

hinge

linker

αD

αE

41 I
84

42 G
92

43 I
93

44 V
94

45 T
95

46 E
96

47 Y
97

48 M
98

49 P
99

50 N
100

51 G
101

52 S
102

53 L
103

54 N
104

55 E
105

56 L
106

57 L
107

58 H
108

59 R
109

60 H
136

•

••

αE

c.l

VII

VIII

61 N
137

62 M
138

63 T
139

64 P
140

65 P
141

66 L
142

67 L
143

68 H
144

69 H
145

70 D
146

71 L
147

72 K
148

73 T
149

74 Q
150

75 N
151

76 I
152

77 L
153

78 L
154

79 I
162

80 A
163

•

DFG

a.l

81 D
164

82 F
165

83 G
166

84 L
167

85 _
_

•

Interaction pattern search

Search KLIFS for kinase-ligand complexes with similar interaction patterns: