KLIFS - the kinase structures database

Version: 3.2
Updated: 29-Oct-2025

B-Raf proto-oncogene, serine/threonine kinase

Kinase structure
Kinase:	BRAF
Family:	RAF
Group:	TKL
Species:	HUMAN
IUPHAR/BPS ID:	1943
PDB:	5HI2
sc-PDB:
KIDFamMap:	Search
Release date:	2016-04-06
PubMed:	26996308
Chain:	A
Orthosteric ligand:	BAX

Structural information
DFG conformation:	out
αC-helix conformation:	in
G-rich loop angle (distance):	58.6° (18.4Å)
G-rich loop rotation:	32.8°
Quality Score:	7.2
Resolution:	2.51 Å
Missing Residues:	0
Missing Atoms:	0

Ligand binding mode
Pockets	Subpockets	Waters
front gate back	BP-I-B BP-II-out BP-III	Cluster O1	Ligand No	Protein Yes

Pocket alignment
Uniprot sequence:	QRIGSGSFGTVYKVAVKMLAFKNEVGVLRKTRVNILLFMGYAIVTQWCEGSSLYHHLHIYLHAKSIIHRDLKSNNIFLIGDFGLA
Sequence structure:	QRIGSGSFGTVYKVAVKMLAFKNEVGVLRKTRVNILLFMGYAIVTQWCEGSSLYHHLHAYLHAKSIIHRDLKSNNIFLIGDFGLS

Ligand affinity
ChEMBL ID:	CHEMBL1336
Bioaffinities:	631 records for 121 kinases

Species	Kinase (ChEMBL naming)	Median	Min	Max	Type	Records
Plasmodium falciparum (isolate 3D7)	Calcium-dependent protein kinase 1	6.7	6.7	6.7	pKd	1
Homo sapiens	CaM kinase II beta	5.4	5.4	5.4	pKi	1
Homo sapiens	Casein kinase I alpha	6.6	6.6	6.6	pIC50	1
Homo sapiens	Cell division cycle 2-like protein kinase 6	6.7	6.7	6.7	pIC50	1
Homo sapiens	Cell division cycle 2-like protein kinase 6	6.6	6.6	6.6	pKd	3
Homo sapiens	Cell division protein kinase 8	5.8	5.3	6.7	pIC50	3
Homo sapiens	Cell division protein kinase 8	6.5	6.5	6.5	pKd	3
Homo sapiens	Cell division protein kinase 8	7.1	7.1	7.1	pKi	1
Homo sapiens	Citron Rho-interacting kinase	5.2	5.2	5.2	pKd	3
Homo sapiens	c-Jun N-terminal kinase 2	5.1	5.1	5.4	pKd	2
Homo sapiens	Cyclin-dependent kinase 2	5.1	5.1	5.1	pKd	1
Homo sapiens	Cyclin-dependent kinase 3	5.4	5.4	5.4	pKd	1
Homo sapiens	Cyclin-dependent kinase 5	5.1	5.1	5.2	pKd	2
Homo sapiens	Cyclin-dependent kinase 7	6.9	6.9	6.9	pKd	3
Homo sapiens	Cyclin-dependent kinase-like 2	6.9	6.9	6.9	pKd	2
Homo sapiens	Cyclin-dependent kinase-like 3	6.3	6.3	6.3	pKd	2
Homo sapiens	Discoidin domain-containing receptor 2	7.4	7.4	8.2	pIC50	2
Homo sapiens	Discoidin domain-containing receptor 2	8.2	6.5	8.2	pKd	6
Homo sapiens	Dual specificity mitogen-activated protein kinase kinase 5	6.7	6.7	6.7	pKd	1
Homo sapiens	Dual specificity protein kinase TTK	5.5	5.5	5.5	pKd	3
Homo sapiens	Dual-specificity tyrosine-phosphorylation regulated kinase 3	5.7	5.7	5.7	pKi	1
Homo sapiens	Dual specificty protein kinase CLK1	5.1	5.1	5.1	pKd	1
Homo sapiens	Ephrin type-A receptor 1	5.5	5.5	5.5	pKd	3
Homo sapiens	Ephrin type-A receptor 2	5.7	5.3	5.7	pKd	4
Homo sapiens	Ephrin type-A receptor 2	6.1	6.1	6.1	pKi	1
Homo sapiens	Ephrin type-A receptor 3	5.7	5.2	5.7	pKd	4
Homo sapiens	Ephrin type-A receptor 4	5.5	5.5	5.9	pKd	4
Homo sapiens	Ephrin type-A receptor 5	6.4	5.5	6.4	pKd	3
Homo sapiens	Ephrin type-A receptor 6	6.4	6.4	6.6	pKd	5
Homo sapiens	Ephrin type-A receptor 7	5.3	5.3	6.2	pKd	5
Homo sapiens	Ephrin type-A receptor 8	5.6	5.6	6	pKd	5
Homo sapiens	Ephrin type-B receptor 1	5.5	5.5	5.8	pKd	4
Homo sapiens	Ephrin type-B receptor 2	5.7	5.7	5.7	pKd	3
Homo sapiens	Ephrin type-B receptor 4	8.3	5.7	9.7	pIC50	5
Homo sapiens	Ephrin type-B receptor 4	5.8	5.4	5.8	pKd	4
Homo sapiens	Epidermal growth factor receptor erbB1	7.1	7.1	8.5	pIC50	2
Homo sapiens	Epithelial discoidin domain-containing receptor 1	8.8	8.8	8.8	pKd	4
Homo sapiens	Fibroblast growth factor receptor 1	6.2	6.2	8.3	pIC50	2
Homo sapiens	Fibroblast growth factor receptor 1	5.6	5.6	5.6	pKd	4
Homo sapiens	Fibroblast growth factor receptor 2	5.6	5.6	5.6	pKd	3
Homo sapiens	Fibroblast growth factor receptor 3	5.2	5.2	5.4	pKd	4
Homo sapiens	Fibroblast growth factor receptor 3	5.9	5.9	5.9	pKi	1
Homo sapiens	Homeodomain-interacting protein kinase 1	5.5	5.5	6	pKd	2
Homo sapiens	Homeodomain-interacting protein kinase 2	5.8	5.8	5.8	pKd	2
Homo sapiens	Homeodomain-interacting protein kinase 2	6.3	6.3	6.3	pKi	1
Homo sapiens	Homeodomain-interacting protein kinase 3	6.4	6.4	6.4	pKd	2
Homo sapiens	Homeodomain-interacting protein kinase 4	8.5	8.5	8.6	pKd	2
Homo sapiens	Homeodomain-interacting protein kinase 4	8	8	8	pKi	1
Homo sapiens	Insulin receptor	5.3	5.3	5.3	pIC50	1
Homo sapiens	Interleukin-1 receptor-associated kinase 1	5.1	5.1	5.1	pKi	1
Homo sapiens	LIM domain kinase 1	5.8	5.7	5.8	pKd	4
Homo sapiens	LIM domain kinase 1	6.2	6.2	6.2	pKi	1
Homo sapiens	LIM domain kinase 2	5	5	5	pKd	1
Homo sapiens	Macrophage colony stimulating factor receptor	7.2	7.2	7.7	pIC50	2
Homo sapiens	Macrophage colony stimulating factor receptor	7.6	7.6	7.6	pKd	3
Homo sapiens	Macrophage colony stimulating factor receptor	6.8	6.8	6.8	pKi	1
Homo sapiens	MAP kinase ERK1	7.7	7.7	7.7	pIC50	1
Homo sapiens	MAP kinase ERK2	7	7	7	pIC50	1
Homo sapiens	MAP kinase-interacting serine/threonine-protein kinase MNK1	6.6	6.6	6.6	pKd	3
Homo sapiens	MAP kinase p38 alpha	7.1	5.5	7.4	pIC50	7
Homo sapiens	MAP kinase p38 alpha	6.4	6.4	6.6	pKd	5
Homo sapiens	MAP kinase p38 alpha	7.8	7.8	7.8	pKi	1
Homo sapiens	MAP kinase p38 beta	6.6	6.6	6.7	pKd	5
Homo sapiens	MAP kinase p38 delta	5.2	5.2	5.2	pKd	2
Homo sapiens	MAP kinase p38 gamma	5.1	5	5.1	pKd	4
Homo sapiens	MAP kinase signal-integrating kinase 2	6.6	6.6	6.6	pIC50	1
Homo sapiens	MAP kinase signal-integrating kinase 2	6.9	6.6	6.9	pKd	4
Homo sapiens	MAP kinase signal-integrating kinase 2	6.5	6.5	6.5	pKi	1
Homo sapiens	Misshapen-like kinase 1	5.8	5.8	5.8	pKi	1
Homo sapiens	Mitogen-activated protein kinase 15	7.3	7.3	7.3	pKd	3
Homo sapiens	Mitogen-activated protein kinase kinase kinase 7	6.2	5.9	6.2	pKd	4
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 1	5.3	5.3	5.3	pKd	1
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 4	5.3	5.3	5.3	pKd	3
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 5	5.8	5.8	5.8	pKd	4
Homo sapiens	Mitogen-activated protein kinase kinase kinase kinase 5	6.5	6.5	6.5	pKi	1
Homo sapiens	Mixed lineage kinase 7	8.2	8.2	8.2	pKd	3
Homo sapiens	Mixed lineage kinase 7	7.8	7.8	7.8	pKi	1
Homo sapiens	Muscle, skeletal receptor tyrosine protein kinase	6.9	6.9	6.9	pKd	3
Homo sapiens	Myosin-IIIB	5.2	5.2	5.2	pKd	1
Homo sapiens	Myosin light chain kinase	5	5	5	pKd	1
Homo sapiens	myosin light chain kinase 2	5.3	5.3	5.9	pKd	2
Homo sapiens	Nerve growth factor receptor Trk-A	5.2	5.2	5.4	pKd	4
Homo sapiens	Nerve growth factor receptor Trk-A	5.8	5.8	5.8	pKi	1
Homo sapiens	Neurotrophic tyrosine kinase receptor type 2	5.7	5.7	5.7	pKd	3
Homo sapiens	Neurotrophic tyrosine kinase receptor type 2	6.2	6.2	6.2	pKi	1
Homo sapiens	Non-receptor tyrosine-protein kinase TNK1	5.6	5.6	5.6	pKd	3
Homo sapiens	NT-3 growth factor receptor	6.2	6.2	6.2	pKd	3
Homo sapiens	NT-3 growth factor receptor	6.4	6.4	6.4	pKi	1
Homo sapiens	Platelet-derived growth factor receptor alpha	7.2	7.2	7.2	pKd	3
Homo sapiens	Platelet-derived growth factor receptor alpha	6	6	6	pKi	1
Homo sapiens	Platelet-derived growth factor receptor beta	7.4	7.2	8	pIC50	5
Homo sapiens	Platelet-derived growth factor receptor beta	7.4	7.4	7.4	pKd	5
Homo sapiens	Platelet-derived growth factor receptor beta	8.4	8.4	8.4	pKi	1
Homo sapiens	Proto-oncogene tyrosine-protein kinase MER	5.4	5.4	5.4	pKd	3
Homo sapiens	Ribosomal protein S6 kinase 1	6.4	6.4	6.4	pKi	1
Homo sapiens	Ribosomal protein S6 kinase alpha 6	5.1	5.1	5.1	pKd	6
Homo sapiens	Serine/threonine-protein kinase 10	6.8	6.8	6.9	pKd	5
Homo sapiens	Serine/threonine-protein kinase 2	6	5.8	6	pKd	4
Homo sapiens	Serine/threonine-protein kinase 2	5.8	5.8	5.8	pKi	1
Homo sapiens	Serine/threonine-protein kinase 33	5.6	5.6	5.6	pKd	3
Homo sapiens	Serine/threonine-protein kinase 36	5.4	5.3	5.4	pKd	4
Homo sapiens	Serine/threonine-protein kinase A-Raf	8.2	8.2	8.2	pIC50	1
Homo sapiens	Serine/threonine-protein kinase Aurora-A	5.4	5.4	5.4	pIC50	4
Homo sapiens	Serine/threonine-protein kinase Aurora-A	6.1	6.1	6.1	pKi	1
Homo sapiens	Serine/threonine-protein kinase Aurora-B	6.4	6.4	6.4	pKd	3
Homo sapiens	Serine/threonine-protein kinase Aurora-B	6.7	6.7	6.7	pKi	1
Homo sapiens	Serine/threonine-protein kinase Aurora-C	6.7	6.7	6.7	pKd	3
Homo sapiens	Serine/threonine-protein kinase B-raf	8.5	8.5	8.5	pEC50	1
Homo sapiens	Serine/threonine-protein kinase B-raf	7.4	5.1	9	pIC50	43
Homo sapiens	Serine/threonine-protein kinase B-raf	6.6	6.3	6.6	pKd	7
Homo sapiens	Serine/threonine-protein kinase B-raf	7.4	7.4	7.7	pKi	2
Mus musculus	Serine/threonine-protein kinase B-raf	6.9	6.9	6.9	pIC50	1
Homo sapiens	Serine/threonine protein kinase NLK	6.2	6.2	6.2	pKd	3
Homo sapiens	Serine/threonine-protein kinase PCTAIRE-2	5.8	5.8	5.8	pKd	3
Homo sapiens	Serine/threonine-protein kinase PFTAIRE-1	5.5	5.5	5.5	pKd	3
Homo sapiens	Serine/threonine-protein kinase PFTAIRE-2	5.5	5.5	5.5	pKd	2
Homo sapiens	Serine/threonine-protein kinase PLK4	5.4	5.4	5.5	pKd	4
Homo sapiens	Serine/threonine-protein kinase PLK4	6.4	6.4	6.4	pKi	1
Homo sapiens	Serine/threonine-protein kinase RAF	7.9	6.4	9	pIC50	22
Homo sapiens	Serine/threonine-protein kinase RAF	6.6	6.6	6.6	pKd	3
Homo sapiens	Serine/threonine-protein kinase RAF	8.2	8.2	8.2	pKi	1
Homo sapiens	Serine/threonine-protein kinase RIPK2	5.4	5.4	5.4	pIC50	1
Homo sapiens	Serine/threonine-protein kinase RIPK2	5.9	5.5	5.9	pKd	4
Homo sapiens	Serine/threonine-protein kinase SRPK1	5.3	5.3	5.3	pKi	1
Homo sapiens	Serine/threonine-protein kinase TAO1	5.5	5.5	6.3	pKd	2
Homo sapiens	Serine/threonine-protein kinase TAO1	6.4	6.4	6.4	pKi	1
Homo sapiens	Serine/threonine-protein kinase TAO2	6.3	6.3	6.3	pKd	1
Homo sapiens	Serine/threonine-protein kinase TAO3	5.6	5.6	5.6	pKd	2
Homo sapiens	Serine/threonine-protein kinase TNNI3K	6.6	6.6	6.6	pKd	3
Homo sapiens	SPS1/STE20-related protein kinase YSK4	7	7	7	pKd	2
Homo sapiens	Stem cell growth factor receptor	7.2	7.2	8	pIC50	4
Homo sapiens	Stem cell growth factor receptor	7.5	6.1	8	pKd	23
Homo sapiens	TGF-beta receptor type II	5.2	5.2	5.2	pKd	1
Homo sapiens	TRAF2- and NCK-interacting kinase	5.1	5.1	5.1	pKd	1
Homo sapiens	Tyrosine-protein kinase ABL	6.9	6.9	7.1	pIC50	2
Homo sapiens	Tyrosine-protein kinase ABL	6.2	5.2	6.9	pKd	40
Homo sapiens	Tyrosine-protein kinase ABL	6.8	6.8	6.8	pKi	1
Mus musculus	Tyrosine-protein kinase ABL	6.7	6.7	6.7	pIC50	1
Homo sapiens	Tyrosine-protein kinase ABL2	5.5	5.5	5.9	pKd	4
Homo sapiens	Tyrosine-protein kinase BLK	6.1	6.1	6.1	pKi	1
Homo sapiens	Tyrosine-protein kinase CSK	5.5	5.5	5.5	pKd	1
Homo sapiens	Tyrosine-protein kinase FGR	5.1	5.1	5.1	pKd	1
Homo sapiens	Tyrosine-protein kinase FRK	6.3	6.3	6.4	pKd	5
Homo sapiens	Tyrosine-protein kinase FYN	5.1	5.1	5.1	pKd	1
Homo sapiens	Tyrosine-protein kinase HCK	6.3	6.3	6.3	pIC50	2
Homo sapiens	Tyrosine-protein kinase HCK	5.1	5.1	5.1	pKd	2
Homo sapiens	Tyrosine-protein kinase JAK3	5.1	5.1	5.1	pKd	2
Homo sapiens	Tyrosine-protein kinase LCK	7.2	7.2	7.2	pIC50	1
Homo sapiens	Tyrosine-protein kinase LCK	5.6	5.2	5.6	pKd	4
Homo sapiens	Tyrosine-protein kinase Lyn	5.5	5.5	5.5	pKd	3
Homo sapiens	Tyrosine-protein kinase Lyn	6.4	6.4	6.4	pKi	1
Homo sapiens	Tyrosine-protein kinase receptor FLT3	7.5	7.2	8.7	pIC50	13
Homo sapiens	Tyrosine-protein kinase receptor FLT3	7.8	7.1	8.4	pKd	23
Homo sapiens	Tyrosine-protein kinase receptor RET	8.1	8.1	8.2	pIC50	2
Homo sapiens	Tyrosine-protein kinase receptor RET	7.9	7.4	8.1	pKd	10
Homo sapiens	Tyrosine-protein kinase receptor RET	7	7	7	pKi	1
Homo sapiens	Tyrosine-protein kinase receptor Tie-1	7.2	7.2	7.2	pKd	3
Homo sapiens	Tyrosine-protein kinase receptor UFO	5.4	5.4	5.4	pKd	3
Homo sapiens	Tyrosine-protein kinase receptor UFO	6.8	6.8	6.8	pKi	1
Homo sapiens	Tyrosine-protein kinase SRC	6.4	6.4	6.4	pIC50	1
Homo sapiens	Tyrosine-protein kinase Srms	5	5	5	pKd	3
Homo sapiens	Tyrosine-protein kinase TIE-2	8.2	8.1	9.1	pIC50	3
Homo sapiens	Tyrosine-protein kinase TIE-2	5.7	5.5	5.7	pKd	4
Homo sapiens	Vascular endothelial growth factor receptor 1	7.5	6.8	7.8	pIC50	3
Homo sapiens	Vascular endothelial growth factor receptor 1	7.5	7.5	7.5	pKd	3
Homo sapiens	Vascular endothelial growth factor receptor 1	6.5	6.5	6.5	pKi	1
Danio rerio	Vascular endothelial growth factor receptor 2	5.6	5.6	5.6	pIC50	1
Homo sapiens	Vascular endothelial growth factor receptor 2	6.3	6.3	6.3	pEC50	1
Homo sapiens	Vascular endothelial growth factor receptor 2	7.2	6	9.8	pIC50	50
Homo sapiens	Vascular endothelial growth factor receptor 2	7.2	7	7.5	pKd	6
Homo sapiens	Vascular endothelial growth factor receptor 2	7.7	7.4	10.7	pKi	4
Mus musculus	Vascular endothelial growth factor receptor 2	7.8	7.8	7.8	pIC50	1
Homo sapiens	Vascular endothelial growth factor receptor 3	7.8	7.8	8.5	pIC50	2
Homo sapiens	Vascular endothelial growth factor receptor 3	7	7	7.8	pKd	5
Homo sapiens	Vascular endothelial growth factor receptor 3	7	7	7	pKi	1

Kinase-ligand interaction pattern

• Hydrophobic • Aromatic face-to-face • Aromatic face-to-edge • H-bond donor • H-bond acceptor • Ionic positive • Ionic negative

g.l

III

αC

1 Q
461

2 R
462

3 I
463

4 G
464

5 S
465

6 G
466

7 S
467

8 F
468

9 G
469

10 T
470

11 V
471

12 Y
472

13 K
473

14 V
480

15 A
481

16 V
482

17 K
483

18 M
484

19 L
485

20 A
497

•

αC

b.l

21 F
498

22 K
499

23 N
500

24 E
501

25 V
502

26 G
503

27 V
504

28 L
505

29 R
506

30 K
507

31 T
508

32 R
509

33 V
511

34 N
512

35 I
513

36 L
514

37 L
515

38 F
516

39 M
517

40 G
518

••

•

hinge

linker

αD

αE

41 Y
519

42 A
526

43 I
527

44 V
528

45 T
529

46 Q
530

47 W
531

48 C
532

49 E
533

50 G
534

51 S
535

52 S
536

53 L
537

54 Y
538

55 H
539

56 H
540

57 L
541

58 H
542

59 A
543

60 Y
566

•

••

•••

αE

c.l

VII

VIII

61 L
567

62 H
568

63 A
569

64 K
570

65 S
571

66 I
572

67 I
573

68 H
574

69 R
575

70 D
576

71 L
577

72 K
578

73 S
579

74 N
580

75 N
581

76 I
582

77 F
583

78 L
584

79 I
592

80 G
593

•

DFG

a.l

81 D
594

82 F
595

83 G
596

84 L
597

85 S
616

••

Interaction pattern search

Search KLIFS for kinase-ligand complexes with similar interaction patterns: