KLIFS - the kinase structures database

Version: 3.2
Updated: 29-Oct-2025

Protein kinase AMP-activated catalytic subunit alpha 2

Kinase structure
Kinase:	PRKAA2 (AMPKa2)
Family:	CAMKL
Group:	CAMK
Species:	HUMAN
IUPHAR/BPS ID:	1542
PDB:	3AQV
sc-PDB:
KIDFamMap:	Search
Release date:	2011-04-27
PubMed:	21543851
Chain:	A
Orthosteric ligand:	TAK

Structural information
DFG conformation:	out-like
αC-helix conformation:	out
G-rich loop angle (distance):	53.7° (16.2Å)
G-rich loop rotation:	96.6°
Quality Score:	8
Resolution:	2.08 Å
Missing Residues:	0
Missing Atoms:	0

Ligand binding mode
Pockets	Subpockets	Waters
front		Cluster I10 I11	Ligand Yes Yes	Protein Yes Yes

Pocket alignment
Uniprot sequence:	DTLGVGTFGKVKIVAVKILKIKREIQNLKLFRPHIIKLYQVFMVMEYVSGGELFDYICKYCHRHMVVHRDLKPENVLLIADFGLS
Sequence structure:	DTLGVGTFGKVKIVAVKILKIKREIQNLKLFRPHIIKLYQVFMVMEYVSGGELFDYICKYCHRHMVVHRDLKPENVLLIADFGLS

Ligand affinity
ChEMBL ID:	CHEMBL478629
Bioaffinities:	28 records for 18 kinases

Species	Kinase (ChEMBL naming)	Median	Min	Max	Type	Records
Homo sapiens	Activin receptor type-1	6.8	6.8	7.2	pIC50	2
Homo sapiens	Activin receptor type-1B	4.6	4.6	4.6	pIC50	1
Homo sapiens	AMP-activated protein kinase, beta-1 subunit	6.5	6.5	6.6	pIC50	2
Homo sapiens	Bone morphogenetic protein receptor type-1A	7	7	7	pIC50	1
Homo sapiens	Bone morphogenetic protein receptor type-1B	6.3	6.3	6.6	pIC50	2
Homo sapiens	Bone morphogenetic protein receptor type-2	7.1	7.1	7.1	pIC50	1
Homo sapiens	Ephrin type-A receptor 2	8	8	8	pIC50	1
Homo sapiens	Interferon-induced, double-stranded RNA-activated protein kinase	4.8	4.8	4.8	pIC50	1
Homo sapiens	MAP kinase-interacting serine/threonine-protein kinase MNK1	8	8	8	pIC50	1
Homo sapiens	Ribosomal protein S6 kinase alpha 1	6.7	6.7	6.7	pIC50	2
Homo sapiens	Serine/threonine-protein kinase receptor R3	7	7	7	pIC50	1
Homo sapiens	TGF-beta receptor type I	5	4.8	5	pIC50	3
Homo sapiens	TGF-beta receptor type II	7	7	7	pIC50	1
Homo sapiens	Tyrosine-protein kinase LCK	7.8	7.8	7.8	pIC50	1
Homo sapiens	Tyrosine-protein kinase SRC	8.7	8.7	8.7	pIC50	1
Homo sapiens	Tyrosine-protein kinase YES	6.7	6.7	7.5	pIC50	2
Homo sapiens	Vascular endothelial growth factor receptor 1	8	8	8	pIC50	1
Homo sapiens	Vascular endothelial growth factor receptor 2	7.6	6.7	8.4	pIC50	4

Kinase-ligand interaction pattern

• Hydrophobic • Aromatic face-to-face • Aromatic face-to-edge • H-bond donor • H-bond acceptor • Ionic positive • Ionic negative

g.l

III

αC

1 D
20

2 T
21

3 L
22

4 G
23

5 V
24

6 G
25

7 T
26

8 F
27

9 G
28

10 K
29

11 V
30

12 K
31

13 I
32

14 V
42

15 A
43

16 V
44

17 K
45

18 I
46

19 L
47

20 K
60

•

αC

b.l

21 I
61

22 K
62

23 R
63

24 E
64

25 I
65

26 Q
66

27 N
67

28 L
68

29 K
69

30 L
70

31 F
71

32 R
72

33 P
74

34 H
75

35 I
76

36 I
77

37 K
78

38 L
79

39 Y
80

40 Q
81

•

hinge

linker

αD

αE

41 V
82

42 F
90

43 M
91

44 V
92

45 M
93

46 E
94

47 Y
95

48 V
96

49 S
97

50 G
98

51 G
99

52 E
100

53 L
101

54 F
102

55 D
103

56 Y
104

57 I
105

58 C
106

59 K
107

60 Y
129

•

••

•

αE

c.l

VII

VIII

61 C
130

62 H
131

63 R
132

64 H
133

65 M
134

66 V
135

67 V
136

68 H
137

69 R
138

70 D
139

71 L
140

72 K
141

73 P
142

74 E
143

75 N
144

76 V
145

77 L
146

78 L
147

79 I
155

80 A
156

•

DFG

a.l

81 D
157

82 F
158

83 G
159

84 L
160

85 S
161

Interaction pattern search

Search KLIFS for kinase-ligand complexes with similar interaction patterns: